FlyBase Allele Report: Dmel\Rab2[Q65L.UASp.YFP]

General Information

Symbol

Dmel\Rab2^{Q65L.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215385

Feature type

allele

Associated gene

Dmel\Rab2

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab2.Q65L}

Key Links

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab2.Q65L}

Cytology

Description

Amino acid replacement: Q65L.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted constitutively active form of Rab2 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab2

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^A53T.UAS

(Szinyákovics et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color | adult stage, with Scer\GAL4^GMR.PF

(Lőrincz et al., 2017)

increased rate of adult locomotory behavior | progressive, with Scer\GAL4^Ddc.PL

(Szinyákovics et al., 2023)

long lived, with Scer\GAL4^Ddc.PL

(Szinyákovics et al., 2023)

visible | adult stage, with Scer\GAL4^GMR.PF

(Lőrincz et al., 2017)

Phenotype Manifest In

autophagosome | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

embryonic/larval fat body | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

embryonic/larval midgut cell | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

eye, with Scer\GAL4^GMR.PF

(Lőrincz et al., 2017)

lysosome | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

retinal pigment granule | adult stage, with Scer\GAL4^GMR.PF

(Lőrincz et al., 2017)

Detailed Description

Statement

Reference

Clones of fat as well as midgut cells expressing Rab2^{Q65L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Act.PU contain enlarged autophagic structures and lysosomes compared to the surrounding control cells. Scer\GAL4^GMR.PF-driven expression of Rab2^{Q65L.Scer\UAS.P\T.T:Avic\GFP-YFP} leads to slightly darker adult eye color and enlarged pigment granules in the retina.

(Lőrincz et al., 2017)

Expression via Scer\GAL4^pnr-MD237 results in flies with a wild type notum.

(Thomas et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rab2^{Q65L.UASp.YFP}, Scer\GAL4^Ddc.PL is a suppressor | partially of short lived phenotype of Hsap\SNCA^A53T.UAS, Scer\GAL4^Ddc.PL

(Szinyákovics et al., 2023)

Rab2^{Q65L.UASp.YFP}, Scer\GAL4^Ddc.PL is a suppressor | partially of decreased rate of adult locomotory behavior | progressive phenotype of Hsap\SNCA^A53T.UAS, Scer\GAL4^Ddc.PL

(Szinyákovics et al., 2023)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rab2^{Q65L.UASp.YFP}, Scer\GAL4^Act.PU has embryonic/larval fat body | somatic clone | larval stage phenotype, suppressible | somatic clone by Rab7^GD11800, Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

Rab2^{Q65L.UASp.YFP}, Scer\GAL4^Act.PU has autophagosome | somatic clone | larval stage phenotype, suppressible | somatic clone by Rab7^GD11800, Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of abnormally large autophagic structures in the clones of larval fat body cells expressing Rab2^{Q65L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Act.PU is prevented by co-expression of Rab7^GD11800.

(Lőrincz et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9760

y¹ w^*; P{UASp-YFP.Rab2.Q65L}02

9761

y¹ w^*; P{UASp-YFP.Rab2.Q65L}25

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab2^{Q65L.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab2^{Q65L.UASp.YFP}

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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