FlyBase Gene Report: Dmel\Rab4

General Information

Symbol

Dmel\Rab4

Species

D. melanogaster

Name

Rab4

Annotation Symbol

CG4921

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016701

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Rab4 (Rab4) encodes a member of the Rab family, which are small Ras-like GTPases involved in vesicle trafficking. The product of Rab4 controls endocytic recycling and regulates synapse organization and neurotransmission in the nervous system. It also contributes to the sorting of cell adhesion molecules during epithelial morphogenesis and immune-mediators during the innate immune response. [Date last reviewed: 2018-09-20] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DmRab4, Rab-protein 4

Key Links

Genomic Location

Cytogenetic map

54C9-54C10

Sequence location

2R:17,573,462..17,574,979 [+]

Recombination map

2-83

RefSeq locus

NT_033778 REGION:17573462..17574979

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:Vps52; FB:FBgn0031710

(Gillingham et al., 2014)

inferred from physical interaction with FLYBASE:CG31064; FB:FBgn0051064

(Gillingham et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR041819

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in follicle cell of egg chamber-cell adhesion

inferred from mutant phenotype

(Chen et al., 2012)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in Rab protein signal transduction

inferred from electronic annotation with InterPro:IPR041819

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

involved_in regulation of endocytosis

inferred from biological aspect of ancestor with PANTHER:PTN001530980

(GO Reference Genome Project, 2011-)

involved_in vesicle-mediated transport

inferred from biological aspect of ancestor with PANTHER:PTN004663565

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in endosome

inferred from direct assay

(Gillingham et al., 2014)

located_in neuronal cell body

inferred from direct assay

(Jin et al., 2012)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

located_in recycling endosome

inferred from direct assay

(West et al., 2015)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

inferred from direct assay

(West et al., 2015, Jin et al., 2012)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in recycling endosome

inferred from biological aspect of ancestor with PANTHER:PTN000635936

(GO Reference Genome Project, 2011-)

located_in vesicle

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

RAB GTPASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Snapshot

Gene Group (FlyBase)

RAB GTPASES -: The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).

Summary (Interactive Fly)

Rab family GTPase endosomal recycling at synapses - maintains gap junctions upon elevated insulin in cultured human cells and in flies - wingless signaling - Pkaap regulates Rab4/Rab11-dependent traffic and Rab11 exocytosis of innate immune cargo - epithelial morphogenesis of leg discs

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rab4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KY04)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rab4-RA

FBtr0086830

NM_166240

1316

213

Rab4-RB

FBtr0086829

NM_079053

973

213

Additional Transcript Data and Comments

Reported size (kB)

0.807, 0.8 (longest cDNA)

(Satoh et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Rab4-PA

FBpp0086009

23.6

213

6.77

FBpp0086008

23.6

213

6.77

Q7KY04

NP_523777

AAM68480

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

213 aa isoforms: Rab4-PA, Rab4-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

197 (aa)

(Satoh et al., 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab4 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.50

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in endosome

inferred from direct assay

(Gillingham et al., 2014)

located_in neuronal cell body

inferred from direct assay

(Jin et al., 2012)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

located_in recycling endosome

inferred from direct assay

(West et al., 2015)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

inferred from direct assay

(West et al., 2015, Jin et al., 2012)

Expression Deduced from Reporters

Reporter: PBac{I-SceI(FRT.Rab4-GAL4.ATG(loxP.3xP3-RFP))}

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6 -- P7

neuropil glial sheath

Comment: 30-40h APF

(Jin et al., 2012)

glial cell of second optic chiasma | intense

Comment: 30-40h APF

(Jin et al., 2012)

mushroom body

Comment: 30-40h APF

(Jin et al., 2012)

eye photoreceptor cell

Comment: 30-40h APF

(Jin et al., 2012)

neuron | subset of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rab4 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rab4

View exonic expression by tissue for Dmel\Rab4

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36992/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016701

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016701

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016701

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4921

Flygut - An atlas of the Drosophila adult midgut

FBgn0016701

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rab4

Transgenic constructs containing regulatory region of Rab4

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Rab4^GD11472

viable, with Scer\GAL4^Mef2.PR

Rab4^GD11472

viable, with Scer\GAL4^pnr-MD237

Rab4^GD11472

viable, with Scer\GAL4^tin.CΔ4

Rab4^GD11472

Sterility

Allele

fertile

Rab4^Gal4-KO

Other Phenotypes

Allele

abnormal developmental rate

Rab4^Gal4-KO

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PU

Rab4^SN.UAS.cTa

decreased size | adult stage

Rab4^Gal4-KO

increased size | adult stage, with Df(2R)ED3385

Rab4^Gal4-KO

visible | adult stage

Rab4^Gal4-KO

visible | adult stage, with Df(2R)ED3385

Rab4^Gal4-KO

Phenotype manifest in

Allele

border follicle cell, with Scer\GAL4^slbo.2.6

Rab4^{S22N.UASp.YFP}

epithelium, with Scer\GAL4^ptc-559.1

Rab4^RNAi.UAS.cUa

larval glial cell, with Scer\GAL4^elav.PU

Rab4^SN.UAS.cTa

outer photoreceptor cell | adult stage

Rab4^Gal4-KO

rhabdomere of eye photoreceptor cell | adult stage

Rab4^Gal4-KO

wing

Rab4^Gal4-KO

wing, with Df(2R)ED3385

Rab4^Gal4-KO

wing disc, with Scer\GAL4^ptc-559.1

Rab4^RNAi.UAS.cUa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (50)

Hsap\RAB4B

13 of 14

Yes

Hsap\RAB4A

11 of 14

Yes

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (39)

Rnor\Rab4b

13 of 14

Yes

Rnor\Rab4a

11 of 14

Yes

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (40)

Mmus\Rab4b

13 of 14

Yes

Mmus\Rab4a

11 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (23)

Xtro\rab4b

10 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (53)

Drer\rab4b

13 of 14

Yes

Drer\rab4a

11 of 14

Yes

4 of 14

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (26)

Cele\rab-14

4 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (19)

Agam\AgaP_AGAP004204

11 of 12

Yes

4 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (51)

Atha\ARA-2

1 of 13

Yes

Atha\ARA4

1 of 13

Yes

Atha\ARA6

1 of 13

Yes

Atha\ARA7

1 of 13

Yes

Atha\ArRABA1h

1 of 13

Yes

Atha\AT5G59840

1 of 13

Yes

Atha\ATFP8

1 of 13

Yes

Atha\GB2

1 of 13

Yes

Atha\RA-5

1 of 13

Yes

Atha\RAB1A

1 of 13

Yes

Atha\RAB1C

1 of 13

Yes

Atha\RAB7A

1 of 13

Yes

Atha\RAB7B

1 of 13

Yes

Atha\RAB8

1 of 13

Yes

Atha\RAB8C

1 of 13

Yes

Atha\RAB11c

1 of 13

Yes

Atha\RAB18

1 of 13

Yes

Atha\RABA1b

1 of 13

Yes

Atha\RABA1c

1 of 13

Yes

Atha\RABA1d

1 of 13

Yes

Atha\RABA1e

1 of 13

Yes

Atha\RABA1f

1 of 13

Yes

Atha\RABA1g

1 of 13

Yes

Atha\RABA1i

1 of 13

Yes

Atha\RABA2b

1 of 13

Yes

Atha\RABA2c

1 of 13

Yes

Atha\RABA2D

1 of 13

Yes

Atha\RABA3

1 of 13

Yes

Atha\RABA4a

1 of 13

Yes

Atha\RABA4B

1 of 13

Yes

Atha\RABA4C

1 of 13

Yes

Atha\RABA4D

1 of 13

Yes

Atha\RABA5a

1 of 13

Yes

Atha\RABA5b

1 of 13

Yes

Atha\RABA5d

1 of 13

Yes

Atha\RABA5E

1 of 13

Yes

Atha\RABA6a

1 of 13

Yes

Atha\RABA6b

1 of 13

Yes

Atha\RABB1a

1 of 13

Yes

Atha\RABB1C

1 of 13

Yes

Atha\RABC2A

1 of 13

Yes

Atha\RABE1c

1 of 13

Yes

Atha\RABE1e

1 of 13

Yes

Atha\RABG1

1 of 13

Yes

Atha\RABG3A

1 of 13

Yes

Atha\RABG3B

1 of 13

Yes

Atha\RABG3c

1 of 13

Yes

Atha\RABG3d

1 of 13

Yes

Atha\RABG3E

1 of 13

Yes

Atha\RABH1e

1 of 13

Yes

Atha\RHA1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\GTR1

1 of 13

Yes

Scer\GTR2

1 of 13

Yes

Scer\SEC4

1 of 13

Yes

Scer\VPS21

1 of 13

Yes

Scer\YPT1

1 of 13

Yes

Scer\YPT6

1 of 13

Yes

Scer\YPT7

1 of 13

Yes

Scer\YPT31

1 of 13

Yes

Scer\YPT32

1 of 13

Yes

Scer\YPT52

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (10)

Spom\ypt4

9 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Rab4. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (33)

8 of 13

6 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Rab4^{UAS.mRFP(Unk)}

ameliorates Huntington's disease

modeled by Hsap\HTT^128Q.FL.UAS

(White et al., 2020)

Rab4^UASp.YFP

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^2xAPP.SP.UAS

(Yu et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAB4B; RAB4B, member RAS oncogene family

13 of 14

612945

RAB4A; RAB4A, member RAS oncogene family

11 of 14

179511

RAB14; RAB14, member RAS oncogene family

4 of 14

612673

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Rab4 - CG10646

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab4 - CG14767

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - CG31064

pull down, peptide massfingerprinting, anti tag western blot

(Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014)

Rab4 - CG42394

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - CG5447

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - CG7668

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - Esp

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - Grip84

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab4 - Grip91

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab4 - Gαo

pull down, anti tag western blot

(Purvanov et al., 2010)

Rab4 - MRP

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - Rab11

anti bait coimmunoprecipitation, western blot

(Martin-Peña and Ferrus, 2020)

Rab4 - RhoGAP68F

anti tag coimmunoprecipitation, anti tag western blot

(de Madrid et al., 2015)

Rab4 - Scamp

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - Syb

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - Synj

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab4 - Tecr

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - Vps50

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab4 - Vps51

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab4 - Vps52

pull down, anti tag western blot, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014)

Rab4 - Vps53

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab4 - ccb

anti bait coimmunoprecipitation, western blot

(Martin-Peña and Ferrus, 2020)

Rab4 - dome

anti tag coimmunoprecipitation, western blot

(Chen et al., 2018)

Rab4 - mop

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, pull down

(Chen et al., 2012)

Rab4 - vptr

experimental knowledge based

(Guruharsha et al., 2011)

Rab4 - γTub23C

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

RNA-protein

Physical Interaction

Assay

References

Rab4 - TBPH

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Gbadamosi et al., 2025)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

mop

suppressible

Rab4

(Chen et al., 2012)

SH3PX1

enhanceable

Rab4

(Zhang et al., 2019)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62680

DroID - A comprehensive database of gene and protein interactions.

FBgn0016701

MIST (protein-protein) - An integrated Molecular Interaction Database

36992

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Synthesis of PIPs at the plasma membrane

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-83

Cytogenetic map

54C9-54C10

Sequence location

2R:17,573,462..17,574,979 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

54C9-54C10

Limits computationally determined from genome sequence between P{EP}MESR4^EP386&P{EP}POSH^EP1206 and P{PZ}l(2)10505¹⁰⁵⁰⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-83

(FlyBase, 2016)

2-86.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

8505

w^*; P{UAS-Rab4-mRFP}2

23269

y¹ w^*; P{UASp-YFP.Rab4}09

9768

y¹ w^*; P{UASp-YFP.Rab4.S22N}37

62542

y¹ w¹¹¹⁸; TI{TI}Rab4^EYFP

55497

y¹ w^*; Mi{MIC}Rab4^MI10530

9767

y¹ w^*; P{UASp-YFP.Rab4}Sap-r³²

9770

y¹ w^*; P{UASp-YFP.Rab4.Q67L}fz2²⁸

98567

y¹ w^*; TI{KozakGAL4}Rab4^{CR70620-KO-kG4}

4921R-2

v106651

v24672

w¹¹¹⁸; P{GD11472}v24672

v342599

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD02419}attP40/CyO-GFP

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (49)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD16736

Other clones

FI09101

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016701

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016701

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Farmer et al., 2026, de Madrid et al., 2015)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Kouranti et al., 2006)

dsRNA made from templates generated with primers directed against Rab4 that is transfected into S2 treated with Listeria monocytogenes reveals Rab4 to be involved in Listeria monocytogenes entry.

(Cheng et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG 4921

(Dorer et al., 2006)

CG4921

(Dunst et al., 2015, Hosono et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Sinka et al., 2008, Zhang et al., 2007, Colicelli, 2004, Lee et al., 2003, Verstreken and Bellen, 2000.9.4)

DRAB4

(Kohno et al., 1994)

DmRab4

(Pereira-Leal and Seabra, 2001, Pereira-Leal and Seabra, 2001)

Q7KY04

(Rojas et al., 2012)

RAB4

(Guan et al., 2020)

RAB4c

(Dorer et al., 2006)

Rab4

(Gbadamosi et al., 2025, Davis et al., 2024, Hodgson et al., 2024, Kim et al., 2024, Mira-Osuna and Borgne, 2024, Wilkin et al., 2024, Yu et al., 2024, Bertin et al., 2023, Glashauser et al., 2023, Li et al., 2023, Troost et al., 2023, Tsarouhas et al., 2023, Zhou et al., 2023, Hodgson et al., 2022, Romanova-Michaelides et al., 2022, Camelo and Luschnig, 2021, Hou and Pei, 2021, Kohrs et al., 2021, Ma and Brill, 2021, Scholl et al., 2021, Linnemannstöns et al., 2020, Martin-Peña and Ferrus, 2020, Weiner et al., 2020, White et al., 2020, Witte et al., 2020, Yu et al., 2020, Jiang et al., 2019, Johnson and Andrew, 2019, Krämer et al., 2019, Sivanantharajah et al., 2019, Zhang et al., 2019, Augustin et al., 2018, Gene Disruption Project members, 2018-, Augustin et al., 2017, Coutinho-Budd et al., 2017, Dey et al., 2017, Gao et al., 2017, Jewett et al., 2017, Caviglia et al., 2016, Neyen et al., 2016, Sorvina et al., 2016, Aradska et al., 2015, Chance and Bashaw, 2015, D'Angelo et al., 2015, de Madrid et al., 2015, West et al., 2015, Couturier et al., 2014, de Vreede et al., 2014, Garg and Wu, 2014, Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014, Ling et al., 2014, Thomas and Strutt, 2014, Yashiro et al., 2014, Couturier et al., 2013, Duan et al., 2013, Gradilla and Guerrero, 2013, O'Sullivan et al., 2013, Pepperl et al., 2013, Solis et al., 2013, Song et al., 2013, Yousefian et al., 2013, Armbruster and Luschnig, 2012, Chen et al., 2012, Gault et al., 2012, Giansanti et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Smith et al., 2012, Wang et al., 2012, Callejo et al., 2011, Leventis et al., 2011, Oberegelsbacher et al., 2011, Rodal et al., 2011, Strutt et al., 2011, Uytterhoeven et al., 2011, Yang et al., 2011, Haglund et al., 2010, Janssens et al., 2010, Mattie et al., 2010, Mottola et al., 2010, Pirraglia et al., 2010, Raja et al., 2010, Yogev et al., 2010, Tan et al., 2009, Thomas et al., 2009, Morrison et al., 2008, Seppa et al., 2008, Sinka et al., 2008, Stone et al., 2008, Strutt and Strutt, 2008, Zheng et al., 2008, Dyer et al., 2007, Zhang et al., 2007, Zhang et al., 2007, Bowler et al., 2006, Kouranti et al., 2006, Sweeney et al., 2006, Sweeney et al., 2006, Wu et al., 2005, Stanyon et al., 2004)

rab4

(Meyer et al., 2022, Lammers et al., 2017, van den Brink et al., 2013, Jin et al., 2012, Chan et al., 2011, Assaker et al., 2010, Silies and Klämbt, 2010)

Name Synonyms

Rab-protein 4

(Cammarato et al., 2011)

Rab4

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

Rab_Effectors_interaction_map

interactome

Interaction map generated by purification of most Rab factors, with identification of copurifying effectors by mass spectrometry.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36992

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KY04

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KY04

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36992/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016701

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016701

FlyMine - An integrated database for Drosophila genomics

FBgn0016701

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4921

MARRVEL_MODEL - MARRVEL (model organism gene)

36992

Linkouts

BioGRID - A database of protein and genetic interactions.

62680

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016701

DroID - A comprehensive database of gene and protein interactions.

FBgn0016701

DRSC - Results frm RNAi screens

FBgn0016701

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016701

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016701

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4921

Flygut - An atlas of the Drosophila adult midgut

FBgn0016701

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0016701

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC010138

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

36992

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Synthesis of PIPs at the plasma membrane

References (191)

The following external sites may use different assemblies or annotations than FlyBase.

-1.50

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