FlyBase Gene Report: Dmel\Rab32

General Information

Symbol

Dmel\Rab32

Species

D. melanogaster

Name

Rab32

Annotation Symbol

CG8024

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002567

Gene Model Status

Current

Stock Availability

30 publicly available

Gene Summary

Rab32 (Rab32) encodes a small GTPase that contributes to vesicle trafficking regulation. It is involved in eye development, autophagy and lipid storage. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

ltd, lightoid, Rab-RP1, RabRP1, DmRab32

Key Links

Genomic Location

Cytogenetic map

45B2-45B3

Sequence location

2R:9,177,864..9,186,342 [-]

Recombination map

2-60

RefSeq locus

NT_033778 REGION:9177864..9186342

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (24 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:ca; FB:FBgn0000247

(Ma et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225, InterPro:IPR030697

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR030697

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN001292747

(GO Reference Genome Project, 2011-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in compound eye pigmentation

inferred from genetic interaction with FLYBASE:p; FB:FBgn0086679

(Falcon-Perez et al., 2007)

involved_in eye pigment granule organization

inferred from mutant phenotype

(Ma et al., 2004)

involved_in mitochondrial transport

inferred from mutant phenotype

(Chen et al., 2020)

involved_in ommochrome biosynthetic process

inferred from mutant phenotype

(Harris et al., 2011)

involved_in regulation of autophagy

inferred from mutant phenotype

(Wang et al., 2012)

involved_in regulation of lipid storage

inferred from mutant phenotype

(Wang et al., 2012)

inferred from genetic interaction with FLYBASE:bmm; FB:FBgn0036449

(Wang et al., 2012)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in intracellular protein transport

inferred from biological aspect of ancestor with PANTHER:PTN001292747

(GO Reference Genome Project, 2011-)

involved_in melanosome organization

inferred from biological aspect of ancestor with PANTHER:PTN000636041

(GO Reference Genome Project, 2011-)

involved_in Rab protein signal transduction

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

involved_in vesicle-mediated transport

inferred from electronic annotation with InterPro:IPR030697

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

Cellular Component (11 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in autophagosome

inferred from direct assay

(Wang et al., 2012)

located_in lysosome

inferred from direct assay

(Wang et al., 2012)

is_active_in mitochondrial outer membrane

inferred from direct assay

(Chen et al., 2020)

located_in recycling endosome

inferred from high throughput direct assay

(Chan et al., 2011)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

is_active_in endomembrane system

inferred from biological aspect of ancestor with PANTHER:PTN001292747

(GO Reference Genome Project, 2011-)

located_in Golgi apparatus

inferred from electronic annotation with InterPro:IPR030697

(InterPro Project Members, 2004-)

is_active_in melanosome

inferred from biological aspect of ancestor with PANTHER:PTN000636041

(GO Reference Genome Project, 2011-)

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000636119

(GO Reference Genome Project, 2011-)

is_active_in trans-Golgi network

inferred from biological aspect of ancestor with PANTHER:PTN000636041

(GO Reference Genome Project, 2011-)

located_in trans-Golgi network

inferred from electronic annotation with InterPro:IPR030697

(InterPro Project Members, 2004-)

located_in vesicle

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR030697

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

RAB GTPASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Snapshot

Rab32 (Rab32) encodes a small GTPase that contributes to vesicle trafficking regulation. It is involved in eye development, autophagy and lipid storage. [Date last reviewed: 2019-09-26]

Gene Group (FlyBase)

RAB GTPASES -: The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

ltd: lightoid

Eye color clear, light, translucent yellowish pink. Resembles lt but is lighter, darkens with age. Ocelli colorless; larval Malpighian tubes colorless. Deficient in ommochrome synthesis. Lack detectable levels of 3OH kynurenine despite normal levels of kynurenine hydroxylase activity; administration of 3 hydroxykynurenine without effect (Phillips, Simmons, and Dowman, 1970, Biochem. Genet. 4: 481-87; Sullivan, Kitos, and Sullivan, 1973, Genetics 75: 651-61). Exhibits reduced levels of phenoazinone synthetase, an enzyme involved in the condensation of 3OH kynurenine molecules to xanthommatin, as do other mutants deficient in ommochrome synthesis (Phillips, Forrest, and Kulkarni, 1973, Genetics 73: 45-56). Uptake of kynurenine, which is produced in the fat body, by the Malpighian tubes, where it is converted to 3-hydrokynurenine, is defective; similar defect seen in eye discs (Sullivan and Sullivan, 1975, Biochem. Genet. 13: 603-13). Sullivan and Sullivan postulate that ltd is a transport mutant that prevents the substrate kynurenine from reaching the sites of its conversion to ommochrome. Phenotypic interaction with other eye-color mutants examined by Rudy and Carvalier (1971, J. Hered. 62: 131-34) and by Silva and Mensua, 1985, DIS 61: 156); produces nearly white eyes when combined with either rb or g.

ltd37b

Eye color of newly hatched adult bright red like v, darkens to a color like pr in old flies. Ocelli colorless; larval Malpighian tubes colorless. Viability excellent. RK1.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rab32 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z7S3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.57

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rab32-RB

FBtr0089931

NM_080370

2771

686

Rab32-RC

FBtr0089934

NM_165659

2347

388

Rab32-RD

FBtr0089933

NM_165658

1448

222

Rab32-RE

FBtr0089932

NM_165660

2156

388

Rab32-RH

FBtr0339369

NM_001299309

1284

214

Rab32-RI

FBtr0346635

NM_165663

1811

389

Rab32-RJ

FBtr0346636

NM_001299308

1342

214

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Rab32-PB

FBpp0088870

74.7

686

6.07

FBpp0088873

42.6

388

6.77

FBpp0088872

24.9

222

6.52

FBpp0088871

42.6

388

6.77

FBpp0308460

24.0

214

5.40

FBpp0312215

42.8

389

4.40

FBpp0312216

24.0

214

5.40

A0A0B4LEY0

NP_001286237

AHN56035

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

388 aa isoforms: Rab32-PC, Rab32-PE

214 aa isoforms: Rab32-PH, Rab32-PJ

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab32 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.55

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

embryonic stage 11 -- 12

Malpighian tubule primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

Additional Descriptive Data

Isoform-specific expression patterns are observed. Sex-specific expression of alternative transcripts observed.

(Telonis-Scott et al., 2009)

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in autophagosome

inferred from direct assay

(Wang et al., 2012)

located_in lysosome

inferred from direct assay

(Wang et al., 2012)

is_active_in mitochondrial outer membrane

inferred from direct assay

(Chen et al., 2020)

located_in recycling endosome

inferred from high throughput direct assay

(Chan et al., 2011)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

Expression Deduced from Reporters

Reporter: PBac{I-SceI(FRT.Rab32-GAL4.ex1(loxP.3xP3-RFP))}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron | subset of larval brain

(Chan et al., 2011)

glial cell | subset of larval brain

(Chan et al., 2011)

neuron | subset of larval ventral nerve cord

(Chan et al., 2011)

Bolwig organ | restricted

(Jin et al., 2012)

leg disc | restricted

(Jin et al., 2012)

pupal stage P6 -- P7

neuron | subset of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)

adult stage

neuron | subset of adult brain

(Chan et al., 2011)

neuron of adult optic lobe | faint

(Jin et al., 2012)

adult insulin secreting cell of pars intercerebralis

(Jin et al., 2012)

neuron of lamina

(Jin et al., 2012)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rab32 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rab32

View exonic expression by tissue for Dmel\Rab32

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002567

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35940/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002567

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002567

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002567

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8024

Flygut - An atlas of the Drosophila adult midgut

FBgn0002567

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Rab32

Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rab32

Transgenic constructs containing regulatory region of Rab32

Aberrations (Deficiencies and Duplications) ( 30 )

Inferred from experimentation ( 30 )

Gene not disrupted in

Df(2R)cn1b

(Yannopoulos et al., 1981)

Df(2R)cn1

(Yannopoulos et al., 1981)

Df(2R)cn2b

(Yannopoulos et al., 1981)

Df(2R)cn2

(Yannopoulos et al., 1981)

Df(2R)cn3

(Yannopoulos et al., 1981)

Df(2R)cn4

(Yannopoulos et al., 1981)

Df(2R)cn5

(Yannopoulos et al., 1981)

Df(2R)cn6

(Yannopoulos et al., 1981)

Df(2R)cn7

(Yannopoulos et al., 1981)

Df(2R)cn8

(Yannopoulos et al., 1981)

Df(2R)cn9

(Yannopoulos et al., 1981)

Df(2R)cn10

(Yannopoulos et al., 1981)

Df(2R)cn11

(Yannopoulos et al., 1981)

Df(2R)cn12

(Yannopoulos et al., 1981)

Df(2R)cn13

(Yannopoulos et al., 1981)

Df(2R)cn14

(Yannopoulos et al., 1981)

Df(2R)cn15

(Yannopoulos et al., 1981)

Df(2R)cn16

(Yannopoulos et al., 1981)

Df(2R)cn17

(Yannopoulos et al., 1981)

Df(2R)cn18

(Yannopoulos et al., 1981)

Df(2R)cn19

(Yannopoulos et al., 1981)

Df(2R)cn20

(Yannopoulos et al., 1981)

Df(2R)cn21

(Yannopoulos et al., 1981)

Dp(2;2)Y3b

(Yannopoulos et al., 1981)

Dp(3;3)Y13

(Yannopoulos et al., 1981)

In(2R)cn10

(Yannopoulos et al., 1981)

In(2R)cn13

(Yannopoulos et al., 1981)

T(Y;2)J-D

Tp(2;Y)C

Gene not duplicated in

Dp(2;3)P32

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal

Rab32^ltd-1

viable

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal developmental rate | larval stage | cold sensitive

Rab32^ltd-1

abnormal developmental rate | larval stage | cold sensitive (with Df(2R)BSC279)

Rab32^ltd-1

abnormal eye color

abnormal memory

abnormal neuroanatomy | adult stage

Rab32^unspecified

decreased body size | larval stage, with Scer\GAL4^grh.D4

decreased size | adult stage

Rab32^ltd-1

increased size | adult stage (with Df(2R)BSC279)

Rab32^ltd-1

some die during P-stage

Rab32^ltd-1

visible | adult stage

Rab32^ltd-1

visible | adult stage (with Df(2R)BSC279)

Rab32^ltd-1

visible | recessive

Rab32^unspecified

Phenotype manifest in

Allele

dopaminergic PPL1 neuron

Rab32^unspecified

dopaminergic PPM1 neuron

Rab32^unspecified

eye photoreceptor cell & pigment granule

Rab32^ltd-1

flight muscle cell | adult stage

Rab32^ltd-1

flight muscle cell | adult stage, with Scer\GAL4^Mef2.247

Rab32^UASp.YFP

Malpighian tubule

mitochondrion | adult stage

Rab32^ltd-1

mitochondrion | adult stage, with Scer\GAL4^Mef2.247

Rab32^UASp.YFP

ocellus pigment granule

outer photoreceptor cell | adult stage

Rab32^ltd-1

pigment cell

primary pigment cell pigment granule

Rab32^ltd-1

rhabdomere of eye photoreceptor cell | adult stage

Rab32^ltd-1

secondary pigment cell pigment granule

Rab32^ltd-1

tertiary pigment cell pigment granule

Rab32^ltd-1

wing

Rab32^ltd-1

wing (with Df(2R)BSC279)

Rab32^ltd-1

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (34)

Hsap\RAB32

9 of 14

Yes

Hsap\RAB38

5 of 14

Yes

Hsap\RAB29

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (31)

Rnor\Rab32

10 of 14

Yes

Rnor\Rab38

6 of 14

Yes

Rnor\Rab29

3 of 14

Yes

Rnor\Rab23

2 of 14

Rnor\Rasef

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (31)

Mmus\Rab32

10 of 14

Yes

Mmus\Rab38

7 of 14

Yes

Mmus\Rab29

3 of 14

Yes

Mmus\Rab23

2 of 14

Mmus\Cracr2a

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (29)

Xtro\rab32

6 of 13

Yes

Xtro\rab38

5 of 13

Yes

Xtro\XB5873314

4 of 13

Yes

Xtro\rab29

2 of 13

Yes

Xtro\rab39a

2 of 13

Yes

Xtro\cfap97

1 of 13

Xtro\cldn10

1 of 13

Yes

Xtro\gatad2a

1 of 13

Xtro\nucks1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\rab35l

1 of 13

Xtro\rab35

1 of 13

Xtro\rabl2b

1 of 13

Yes

Xtro\vstm2l

1 of 13

Yes

Danio rerio (Zebrafish) (37)

Drer\rab32a

6 of 14

Yes

Drer\rab38c

6 of 14

Yes

Drer\rab38b

5 of 14

Yes

Drer\zgc:162171

5 of 14

Yes

Drer\rab32b

4 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\glo-1

9 of 14

Yes

Cele\R07B1.8

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP007654

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (37)

Atha\ARA-2

1 of 13

Yes

Atha\ARA4

1 of 13

Yes

Atha\ARA6

1 of 13

Yes

Atha\ARA7

1 of 13

Yes

Atha\ArRABA1h

1 of 13

Yes

Atha\AT5G59840

1 of 13

Yes

Atha\ATFP8

1 of 13

Yes

Atha\GB2

1 of 13

Yes

Atha\RA-5

1 of 13

Yes

Atha\RAB1A

1 of 13

Yes

Atha\RAB1C

1 of 13

Yes

Atha\RAB7A

1 of 13

Yes

Atha\RAB7B

1 of 13

Yes

Atha\RAB8

1 of 13

Yes

Atha\RAB11c

1 of 13

Yes

Atha\RABA1b

1 of 13

Yes

Atha\RABA1c

1 of 13

Yes

Atha\RABA1d

1 of 13

Yes

Atha\RABA1e

1 of 13

Yes

Atha\RABA1f

1 of 13

Yes

Atha\RABA1g

1 of 13

Yes

Atha\RABA1i

1 of 13

Yes

Atha\RABA2b

1 of 13

Yes

Atha\RABA2D

1 of 13

Yes

Atha\RABA4a

1 of 13

Yes

Atha\RABA4B

1 of 13

Yes

Atha\RABA5b

1 of 13

Yes

Atha\RABA5d

1 of 13

Yes

Atha\RABA5E

1 of 13

Yes

Atha\RABB1C

1 of 13

Yes

Atha\RABE1c

1 of 13

Yes

Atha\RABG3A

1 of 13

Yes

Atha\RABG3B

1 of 13

Yes

Atha\RABG3c

1 of 13

Yes

Atha\RABG3d

1 of 13

Yes

Atha\RABG3E

1 of 13

Yes

Atha\RHA1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\YPT1

1 of 13

Yes

Scer\YPT7

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\ypt1

1 of 12

Yes

Spom\ypt2

1 of 12

Yes

Spom\ypt7

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Rab32. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (31)

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Parkinson disease 16 (postulated), RAB29-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Rab32^unspecified

model of Parkinson's disease

CEA

(Macleod et al., 2013)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

Rab32^ltd-1

exacerbates Hermansky-Pudlak syndrome

modeled by Blos1^ex2

(Cheli et al., 2010)

Rab32^UASp.YFP

exacerbates Parkinson's disease 8

modeled by Lrrk¹

(Inoshita et al., 2022)

Rab32^{T33N.UASp.YFP}

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

Rab32^{Q79L.UASp.YFP}

ameliorates Parkinson's disease

modeled by Hsap\LRRK2^{G2019S.UAS.cMa}

(Macleod et al., 2013)

Rab32^KK107695

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAB32; RAB32, member RAS oncogene family

9 of 14

612906

PARKINSON DISEASE 26, AUTOSOMAL DOMINANT, SUSCEPTIBILITY TO; PARK26

RAB38; RAB38, member RAS oncogene family

5 of 14

606281

RAB29; RAB29, member RAS oncogene family

3 of 14

603949

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Rab32 - mir-184

luminiscence technology

(Iovino et al., 2009)

protein-protein

Physical Interaction

Assay

References

Rab32 - Cisd

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot

(Chen et al., 2020)

Rab32 - ca

pull down, anti tag western blot

(Ma et al., 2004)

Rab32 - didum

two hybrid, pull down, anti tag western blot, anti tag coimmunoprecipitation, autoradiography

(Ji et al., 2015, Satoh et al., 2008)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Rab32

enhanceable

aux

(Cheli et al., 2010)

Rab32

enhanceable

Blos1

(Cheli et al., 2010)

Rab32

suppressible

Cisd

(Chen et al., 2020)

Rab32

enhanceable

(Lloyd et al., 2002)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bchs

enhanceable

Rab32

(Simonsen et al., 2007)

Blos1

enhanceable

Rab32

(Cheli et al., 2010)

Cisd

suppressible

Rab32

(Chen et al., 2020)

Cisd

enhanceable

Rab32

(Chen et al., 2020)

enhanceable

Rab32

(Falcon-Perez et al., 2007)

para

suppressible

Rab32

(Kroll et al., 2015)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61778

DroID - A comprehensive database of gene and protein interactions.

FBgn0002567

MIST (protein-protein) - An integrated Molecular Interaction Database

35940

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-60

Cytogenetic map

45B2-45B3

Sequence location

2R:9,177,864..9,186,342 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

45B2-45B3

Limits computationally determined from genome sequence between P{lacW}CG8788^k04512&P{lacW}l(2)k13412^k13412 and P{PZ}Pkn⁰⁶⁷³⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

45A8-45B5

(Ozaki, 1999.12.8)

Experimentally Determined Recombination Data

Location

2-60

(FlyBase, 2016)

2-58.2

(Comeron et al., 2012)

2-[59]

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (30)

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (139)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI09421

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002567

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002567

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Kouranti et al., 2006)

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per⁰¹, tim⁰¹ and Clk^Jrk background.

(Claridge-Chang et al., 2001)

Area matching Drosophila EST AA979014.

(Davies, 2001.3.15)

Used as a marker gene, results of LUG and SIP analysis shows genes conferring resistance to parasitism by A.tabida are restricted to the second chromosome.

(Orr and Irving, 1997)

ltd is required for the synthesis of ommochrome in any tissue and may be a component of the ommochrome precursor transport system.

(Tearle, 1991)

Lesions in ltd reduce or eliminate pigmentation in the eyes and ocelli and block pigmentation of the fat body and tubules.

(Tearle, 1991)

Pteridines (neodrosopterin, drosopterin, isodrosopterin, aurodrosopterin, 6-acetyl-dihydrohomopterin, sepiapterin, dihydrobiopterin, biopterin, pterin and isoxanthopterin) have been quantified in single and double mutants.

(Silva and Mensua, 1985)

It is likely that this gene is required for the normal transport of eye-pigment precursors.

(Silva and Mensua, 1985)

Relationship to Other Genes

Source for database merge of

Source for merge of: ltd Rab-RP1

(Ma et al., 2004)

Source for merge of: ltd anon-WO0118547.106

(FlyBase, 1996-)

Additional comments

Source for merge of ltd anon-WO0118547.106 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Rab32 ltd

(FlyBase, 2009-)

Nomenclature comments

Renamed from 'ltd' to 'Rab32' (a popular synonym) to be consistent with the nomenclature of all other Rab genes.

(FlyBase, 2009-)

Etymology

Given the name "beck", after one of Pavlov's dogs.

(Dubnau et al., 2003)

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

24652026

(Rojas et al., 2012)

24652028

(Rojas et al., 2012)

A1Z7S1

(Rojas et al., 2012)

CG8024

(National Institute of Genetics Fly Stocks, 2022-, Meadows, 2017.1.11, Dunst et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Goldman and Arbeitman, 2007, Muse et al., 2007, Zhang et al., 2007, Colicelli, 2004, Ma et al., 2004, Dubnau et al., 2003, Giot et al., 2003, Mount, 2001.8.14)

DRABR1

(Kohno et al., 1994)

DmRab32

(Pereira-Leal and Seabra, 2001, Pereira-Leal and Seabra, 2001)

DrabRP1

(Satoh et al., 1997)

Ltd

(Ji et al., 2015)

RAB-RP1

(Ganguly-Fitzgerald et al., 2006)

RAB7L

(Xiong and Yu, 2018)

Rab-RP1

(Berger et al., 2008, Ma et al., 2004, Claridge-Chang et al., 2001)

Rab-RP1/CG8024

(Chang et al., 2004)

Rab-r1

Rab32/RP1

(Zhang et al., 2007)

Rab7L1

(Macleod et al., 2013)

RabRP1

(Xu et al., 2004, Fujikawa et al., 2003)

anon-WO0118547.106

beck

(Berger et al., 2008)

ltd

(Rodriguez-Fernandez and Dell'Angelica, 2015, Sanhueza et al., 2015, Ugrankar et al., 2015, Gupta et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Wang et al., 2012, Harris et al., 2011, Napoletano et al., 2011, Cheli et al., 2010, Cheli et al., 2010, Gan et al., 2010, Rodriguez-Fernandez and Dell'angelica, 2009, Telonis-Scott et al., 2009, Satoh et al., 2008, Bakal et al., 2007, Falcon-Perez et al., 2007, Muse et al., 2007, Cherry and Perrimon, 2004)

rab32

(Lammers et al., 2017, Jin et al., 2012, Chan et al., 2011)

Name Synonyms

Lightoid

(Cheli et al., 2010, Satoh et al., 2008)

Rab-related protein 1

Rab32

lightoid

(Macleod et al., 2013, Pandey et al., 2013, Wang et al., 2012, Harris et al., 2011, Yoshimura et al., 2010, Al-Anzi et al., 2009, Rodriguez-Fernandez and Dell'angelica, 2009, Berger et al., 2008, Falcon-Perez et al., 2007, Goldman and Arbeitman, 2007, Simonsen et al., 2007, Cherry and Perrimon, 2004)

Secondary FlyBase IDs

FBgn0015788
FBgn0062240

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

Rab_Effectors_interaction_map

interactome

Interaction map generated by purification of most Rab factors, with identification of copurifying effectors by mass spectrometry.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 79 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35940

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z7S3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z7S3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002567

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35940/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002567

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002567

FlyMine - An integrated database for Drosophila genomics

FBgn0002567

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8024

MARRVEL_MODEL - MARRVEL (model organism gene)

35940

Linkouts

BioGRID - A database of protein and genetic interactions.

61778

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002567

DroID - A comprehensive database of gene and protein interactions.

FBgn0002567

DRSC - Results frm RNAi screens

FBgn0002567

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002567

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002567

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8024

Flygut - An atlas of the Drosophila adult midgut

FBgn0002567

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0002567

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

35940

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (140)

The following external sites may use different assemblies or annotations than FlyBase.

-0.55

Report Sections