FlyBase Allele Report: Dmel\Rab32[Q79L.UASp.YFP]

General Information

Symbol

Dmel\Rab32^{Q79L.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215439

Feature type

allele

Associated gene

Dmel\Rab32

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab32.Q79L}

Key Links

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab32.Q79L}

Cytology

Description

Amino acid replacement: Q79L.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted constitutively active form of Rab32 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab32

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\LRRK2^{G2019S.UAS.cMa}

(Macleod et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing Rab32^{Q79L.UASp.YFP} under the control of Scer\GAL4^Mef2.247 leads to larger mitochondria in adult flight muscles.

(Chen et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rab32^{Q79L.UASp.YFP}, Scer\GAL4^ple.PF is a suppressor | partially of short lived phenotype of Hsap\LRRK2^{G2019S.UAS.cMa}, Scer\GAL4^ple.PF

(Macleod et al., 2013)

Rab32^{Q79L.UASp.YFP}, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\LRRK2^{G2019S.UAS.cMa}, Scer\GAL4^ple.PF

(Macleod et al., 2013)

NOT Suppressor of

Statement

Reference

Rab32^{Q79L.UASp.YFP}/Scer\GAL4^GMR57C10 is a non-suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Rab32^{Q79L.UASp.YFP}/Scer\GAL4^GMR57C10 is a non-suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rab32^{Q79L.UASp.YFP}, Scer\GAL4^Mef2.247 is an enhancer of flight muscle cell | adult stage phenotype of Cisd^UAS.cCa, Scer\GAL4^Mef2.247

(Chen et al., 2020)

Rab32^{Q79L.UASp.YFP}, Scer\GAL4^Mef2.247 is an enhancer of mitochondrion | adult stage phenotype of Cisd^UAS.cCa, Scer\GAL4^Mef2.247

(Chen et al., 2020)

Rab32^{Q79L.UASp.YFP}, Scer\GAL4^Mef2.247 is an enhancer of vesicle | adult stage phenotype of Cisd^UAS.cCa, Scer\GAL4^Mef2.247

(Chen et al., 2020)

Suppressor of

Statement

Reference

Rab32^{Q79L.UASp.YFP}, Scer\GAL4^ple.PF is a suppressor of dopaminergic PPM1 neuron phenotype of Hsap\LRRK2^{G2019S.UAS.cMa}, Scer\GAL4^ple.PF

(Macleod et al., 2013)

Rab32^{Q79L.UASp.YFP}, Scer\GAL4^ple.PF is a suppressor of dopaminergic PPL1 neuron phenotype of Hsap\LRRK2^{G2019S.UAS.cMa}, Scer\GAL4^ple.PF

(Macleod et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rab32^{Q79L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR57C10 does not rescue the bang sensitivity of para^bss1/+ flies, hemizygous para^bss1 flies or eas² mutants.

(Kroll et al., 2015)

Xenogenetic Interactions

Statement

Reference

Co-expression of ltd^{Q79L.Scer\UAS.P\T.T:Avic\GFP-YFP} rescues the reduced lifespan and loss of dorsomedial posterior protocerebral and dorsolateral posterior protocerebral dopaminergic neurons seen in flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.cMa} under the control of Scer\GAL4^ple.PF.

(Macleod et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9816

y¹ w^*; P{UASp-YFP.Rab32.Q79L}sprt⁰⁸

23280

y¹ w^*; P{UASp-YFP.Rab32.Q79L}mRpL19⁰³

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rab32^{Q79L.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab32^{Q79L.UASp.YFP}

ltd^{Q79L.Scer\UAS.P\T.T:Avic\GFP-YFP}

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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