FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ca, Rab32
FlyBase ID
FBig0000120989
Interaction Network
Interactions Browser links
ca network
Rab32 network
Reported Interactions
FBrf0180621-1.PD.WB
Reference
Ma et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab32 

Rab-RP1

bait

Gst-tag

ca 

Claret

prey

HA-tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was Human embryonic kidney 293T cells; bait produced from transfected construct; prey produced from transfected construct.

(Ma et al., 2004)
FBrf0180621-2.PD.WB
Reference
Ma et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ca 

Claret

bait

Gst-tag

ca 
Rab32 

Rab-RP1

prey

HA-tag

Rab32 
Rab32 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ca 
interaction domain
sufficient binding region
aa 1135-1273
Rab32 
nucleotide free mutant
mutation increasing interaction
unspecified

RabRP1 (nucleotide free) contains a single-point mutation equivalent to the N116I mutation in Ras.

Rab32 
GTPase deficient region
mutation decreasing interaction
unspecified

RabRP1 (GTPase deficient) contains a single-point mutation equivalent to the Q61L mutation in Ras.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was Human embryonic kidney 293T cells; bait produced from transfected construct; prey produced from transfected construct.

(Ma et al., 2004)

Claret brought down a nucleotide-free mutant form of Rab-RP1 preferentially over its GTPase-deficient mutant form.

(Ma et al., 2004)
External Crossreferences and Linkouts ( 0 )
References (1)