FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mop, Rab4
FlyBase ID
FBig0000097692
Interaction Network
Interactions Browser links
mop network
Rab4 network
Reported Interactions
FBrf0220289-1.coIP.WB
Reference
Chen et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mop 

Mop

bait

HA tag

mop 
Rab4 

Rab4

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mop 
Bro1 domain
sufficient binding region
aa 1-434
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2012)

mop interacts with Rab4 and Rab5, but not with Rab7 or Rab11.

(Chen et al., 2012)
FBrf0220289-3.Y2H
Reference
Chen et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mop 

Mop

bait

fused to GAL4 DNA-binding domain

mop 
Rab4 

Rab4

prey

fused to GAL4 activation domain

Rab4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mop 
Bro1 domain
sufficient binding region
aa 1-434
Rab4 
region
sufficient binding region
aa 87-213
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Chen et al., 2012)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Chen et al., 2012)
FBrf0220289-4.PD.WB
Reference
Chen et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab4 

Rab4

bait

GST tag

mop 

Mop

prey

HA tag

mop 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mop 
Bro1 domain
sufficient binding region
aa 1-410
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as recombinant fusion protein in HEK293 cell line.

(Chen et al., 2012)

mop interacts with Rab4 in a guanine nucleotide-independent manner.

(Chen et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)