FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rab4S22N.UASp.YFP
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General Information
Symbol
Dmel\Rab4S22N.UASp.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215390
Feature type
allele
Associated gene
Dmel\Rab4
Associated Insertion(s)
Carried in Construct
P{UASp-YFP.Rab4.S22N}
Also Known As
Rab4DN, Rab4DN, UAS-YFP-rab4DN, DN-Rab4
Key Links
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
in vitro construct
(Zhang et al., 2007, Zhang et al., 2007)
Progenitor genotype
Carried in construct
P{UASp-YFP.Rab4.S22N}
Cytology
Description

Amino acid replacement: S22N.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab4 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Rab4
Tagged with
YFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus by adult hemocytes expressing Rab4S22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ are not significantly different from those seen in control hemocytes.

      (Garg and Wu, 2014)

      Expression of Rab4S22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of either Scer\GAL4eg-Mz360, Scer\GAL4eve.RN2 or Scer\GAL4elav-C155 does not result in any defects in axon outgrowth in the embryo.

      (van den Brink et al., 2013)

      Expression of Rab4S22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4slbo.2.6 results in dissociation of the border cell cluster in the developing egg chamber. Border cell migration is unaffected.

      (Chen et al., 2012)

      Expression via Scer\GAL4pnr-MD237 results in flies with a wild type notum.

      (Thomas et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      Rab4S22N.UASp.YFP, Scer\GAL4en.PU is a suppressor of visible phenotype of Scer\GAL4en.PU, mopUAS.cCa

      (Chen et al., 2012)
      NOT Suppressor of
      Phenotype Manifest In
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      Rab4S22N.UASp.YFP, Scer\GAL4en.PU is a suppressor | partially of wing phenotype of Scer\GAL4en.PU, mopUAS.cCa

      (Chen et al., 2012)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Rab4S22N.Scer\UAS.P\T.T:Avic\GFP-YFP partially suppresses the blistered wing phenotype seen when mopScer\UAS.cCa is expressed under the control of Scer\GAL4en.PU.

      (Chen et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rab4S22N.Scer\UAS.P\T.T:Avic\GFP-YFP
      Rab4S22N.UASp.YFP
      Name Synonyms
      Secondary FlyBase IDs
        References (21)