FlyBase Allele Report: Dmel\Ask1[ΔN.UAS.Tag:FLAG]

General Information

Symbol

Dmel\Ask1^{ΔN.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0263920

Feature type

allele

Associated gene

Dmel\Ask1

Associated Insertion(s)

Carried in Construct

P{UAS-Ask1.ΔN}

Key Links

Transgenic product class

inframe_deletion

(Sekine et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Sekine et al., 2011)

Mutagen

in vitro construct

(Sekine et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Ask1.ΔN}

Cytology

Description

UAS drives expression of a cDNA encoding Ask1 that lacks the N-terminal 558 amino acids, with an N-terminal Tag:FLAG tag. This acts as a constitutively active form.

(Sekine et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ask1

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Sekine et al., 2012, Sekine et al., 2011)

abnormal body color | heat sensitive, with Scer\GAL4^hs.PU

(Sekine et al., 2011)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^pnr-MD237

(Sekine et al., 2012)

adult thorax, with Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

adult thorax | heat sensitive, with Scer\GAL4^hs.PU

(Sekine et al., 2011)

wing | heat sensitive, with Scer\GAL4^hs.PU

(Sekine et al., 2011)

Detailed Description

Statement

Reference

Expression of Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237 results in an increase in melanisation of the adult thorax cuticle in the domain of Scer\GAL4^pnr-MD237 expression.

(Sekine et al., 2012)

Flies expressing Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237 exhibit black pigmentation in the thorax cuticle.

Ubiquitous expression of Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^hs.PU, induced by a 1 hour heat shock during the pupal stage, results in black pigmentation only in the lower medial region of the thorax cuticle, together with a defect in wing expansion.

(Sekine et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by p38a^DN.UAS, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by p38a^NIG.5475R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by p38b^NIG.7393R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible | partially by p38c^KK103439, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by lic^GD7546, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible | partially by Mkk4^NIG.9738R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by hep^NIG.4353R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by e^UAS.cWa, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible | partially by y¹

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by ple^RNAi.UAS, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible by Ddc^NIG.10697R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, suppressible | partially by Hr38^RNAi.UAS.cSa, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

NOT suppressed by

Statement

Reference

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, non-suppressible by slim^RNAi.UAS, Scer\GAL4^pnr-MD237

(Sekine et al., 2012)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has abnormal body color phenotype, non-suppressible by hep¹

(Sekine et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible by hep^NIG.4353R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible by e^UAS.cWa, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible | partially by y¹

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible by ple^RNAi.UAS, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible by Ddc^NIG.10697R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible | partially by Hr38^RNAi.UAS.cSa, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible by p38a^DN.UAS, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible by p38a^NIG.5475R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible by p38b^NIG.7393R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible | partially by p38c^KK103439, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible by lic^GD7546, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, suppressible | partially by Mkk4^NIG.9738R, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

NOT suppressed by

Statement

Reference

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, non-suppressible by slim^RNAi.UAS, Scer\GAL4^pnr-MD237

(Sekine et al., 2012)

Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-suppressible by hep¹

(Sekine et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of slim^{dsRNA.Scer\UAS} has no effect on the increase in melanisation of the adult thorax cuticle seen in flies expressing Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^pnr-MD237.

(Sekine et al., 2012)

Co-expression of Mpk2^DN.Scer\UAS, Mpk2^NIG.5475R, p38b^NIG.7393R, or lic^GD7546 abolishes the thorax pigmentation defects seen in Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies. Co-expression of p38c^KK103439 has only a marginal suppressive effect.

Co-expression of Mkk4^NIG.9738R attenuates the thorax pigmentation defects seen in Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies.

Co-expression of hep^NIG.4153R or hemizygosity for hep¹ does not suppress the thorax pigmentation defects seen in Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies.

Co-expression of e^Scer\UAS.cWa abolishes the thorax pigmentation defects seen in Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies.

In a y¹ hemizygous background, pigmentation in the thorax of Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies is brown rather than black, particularly in the scutellum.

Co-expression of either ple^{dsRNA.Scer\UAS} or Ddc^NIG.10697R abolishes the thorax pigmentation defects seen in Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies.

Co-expression of Hr38^{dsRNA.Scer\UAS} reduces the thorax pigmentation defects seen in Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies at 18[o]C.

(Sekine et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ask1^{ΔN.UAS.Tag:FLAG} is rescued by Scer\GAL4^pnr-MD237/Ask1^RNAi.UAS

(Sekine et al., 2011)

Comments

Co-expression of Pk92B^{dsRNA.Scer\UAS} suppresses the pigmentation defect in Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies.

(Sekine et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ask1^{ΔN.Scer\UAS.T:Zzzz\FLAG}

Ask1^{ΔN.UAS.Tag:FLAG}

Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections