Rack1EY00128/+ mutant flies exhibit temperature-induced mobility defects.
Rack1EY00128 mutant germline clones show oocyte mislocalisation.
Although Rack1EE/Rack1EY00128 mutants show larval lethality, up to 35% of animals are escapers. Females at 3 days posteclosion contain ovaries that are reduced in size but have no gross morphological defects. At 7 days, the ovarioles are crowded with small, but mature, stage 14 eggs. Rack1EE/Rack1EY00128 germaria have reduced numbers of germline stem cells but these are never missing completely. The number of germ-line derived cells in the egg chambers of these mutants does not seem affected. Oogenesis can proceed relatively normally to stage 14, but the resulting eggs are not laid and accumulate in the ovariole.
Rack1EY00128/Df(2L)RACK1-mts and Rack1EY00128/Rack11.8 escapers display a range of phenotypes including sluggish movement, difficulty in exiting the pupal case, cuticle that does not harden, and wings that are blistered, abnormally small, or that fail to unfold. Escaper females are incapable of laying eggs and the size of the ovary is reduced compared to wild type but overall body size is unaffected.