Neuromuscular junction 4 (NMJ4) terminals look similar across larval abdominal segments A3-A6 in wild type. However, in Acsl05847/AcslKO mutants, NMJs innervating the anterior segment muscles have shorter axons that are overgrown with more satellite boutons, whereas those innervating the posterior segment muscles have longer axons and are dystrophic with relatively few boutons.
AcslKO mosaic eyes have significantly greater numbers of 'endocytic Rh1 particles' (ERPs) per rhabdomere at 2 and 5 hrs after light exposure, compared to wild type.
AcslKO is an early larval lethal allele.
In the cross section of the larval segmental nerves of AcslKO/Acsl05847 mutants, there are conspicuous axonal swellings packed with heterogeneous membranous organelles. These aggregates, most often observed in posterior axons, include dark, prelysosomal and multivesicular bodies. In some mutant larvae, autophagosomes are also observed where a cluster of large clear-core vesicles are surrounded by a double-layer membrane. The mutant nerves also display lamellated bodies which resemble autolysosomes. In other cases, clusters of large clear-core vesicles are observed. These membrane aggregates are rarely found in wild-type axons.
AcslKO/Acsl05847 mutants display pronounced dystrophy of larval neuromuscular junction 4 (NMJ4) in the posterior abdominal segments A6 and A7, while the muscle and larval size are comparable to wild-type. The bouton number and the synaptic area are significantly reduced in AcslKO/Acsl05847 mutants compared with wild-type.
The synaptic area of AcslKO/Acsl05847 in AcslKO/Acsl05847 mutants is comparable to that of wild-types 2 days after egg laying (AEL). Synapse growth during 2-3.5 days AEL of AcslKO/Acsl05847 mutants is markedly slower than that in wild-types. From 3.5 to 5 days AEL, the mutant NMJ synapses apparently retract.
The evoked excitatory junction potentials (EJPs) are significantly reduced in AcslKO/Acsl05847 mutant larvae recorded from muscle 6 in the posterior abdominal segment. Miniature EJPs (mEJPs, also known as quantal size) are normal in AcslKO/Acsl05847 mutants. Quantal content is significantly reduced in AcslKO/Acsl05847 mutants.