FlyBase Allele Report: Dmel\Acsl[KO]

General Information

Symbol

Dmel\Acsl^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0247206

Feature type

allele

Associated gene

Dmel\Acsl

Associated Insertion(s)

Carried in Construct

Also Known As

dAcsl^KO

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

FLPase

Nature of the Allele

Allele class

loss of function allele

(Zhang et al., 2011)

Mutagen

FLPase

(Zhang and Wang, 2010.11.5)

Progenitor genotype

PBac{RB}Acsl^e02676

(Zhang and Wang, 2010.11.5)

PBac{WH}f02764

(Zhang and Wang, 2010.11.5)

Caused by aberration

Df(2R)Acsl^KO

Cytology

Description

Recombination between the two progenitor insertions has resulted in the deletion of the sequence between them.

(Zhang and Wang, 2010.11.5)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:8,673,192..8,683,622 [+]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has deleted the sequence between them.

(Zhang and Wang, 2010.11.5)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of non-syndromic X-linked intellectual disability 63

CEA with Acsl⁰⁵⁸⁴⁷

(Huang et al., 2016, Liu et al., 2011)

CEA with Acsl^SH0465

(Liu et al., 2011)

model of non-syndromic X-linked intellectual disability

CEA

(Jia et al., 2019)

CEA with Acsl⁰⁵⁸⁴⁷

(Jia et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of non-syndromic X-linked intellectual disability

is ameliorated by Acsl^{UAS.715.C.Tag:MYC}

(Jia et al., 2019)

is ameliorated by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}

(Jia et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014, Liu et al., 2011)

abnormal neurophysiology (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2011)

lethal - all die before end of first instar larval stage | recessive

(Liu et al., 2011)

some die during first instar larval stage | recessive

(Liu et al., 2011)

Phenotype Manifest In

bouton (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2011)

embryonic/larval neuromuscular junction (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2011)

larval abdominal 2 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 3 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 4 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 5 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 6 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 7 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal segment 6 (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2011)

larval abdominal segment 7 (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2011)

larval segmental nerve (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2011)

neuromuscular junction (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

NMJ bouton (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

rhabdomere | somatic clone | conditional

(Liu et al., 2014)

synapse (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2011)

Detailed Description

Statement

Reference

Neuromuscular junction 4 (NMJ4) terminals look similar across larval abdominal segments A3-A6 in wild type. However, in Acsl⁰⁵⁸⁴⁷/Acsl^KO mutants, NMJs innervating the anterior segment muscles have shorter axons that are overgrown with more satellite boutons, whereas those innervating the posterior segment muscles have longer axons and are dystrophic with relatively few boutons.

Acsl^KO mosaic eyes have significantly greater numbers of 'endocytic Rh1 particles' (ERPs) per rhabdomere at 2 and 5 hrs after light exposure, compared to wild type.

(Liu et al., 2014)

Acsl^KO is an early larval lethal allele.

In the cross section of the larval segmental nerves of Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants, there are conspicuous axonal swellings packed with heterogeneous membranous organelles. These aggregates, most often observed in posterior axons, include dark, prelysosomal and multivesicular bodies. In some mutant larvae, autophagosomes are also observed where a cluster of large clear-core vesicles are surrounded by a double-layer membrane. The mutant nerves also display lamellated bodies which resemble autolysosomes. In other cases, clusters of large clear-core vesicles are observed. These membrane aggregates are rarely found in wild-type axons.

Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants display pronounced dystrophy of larval neuromuscular junction 4 (NMJ4) in the posterior abdominal segments A6 and A7, while the muscle and larval size are comparable to wild-type. The bouton number and the synaptic area are significantly reduced in Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants compared with wild-type.

The synaptic area of Acsl^KO/Acsl⁰⁵⁸⁴⁷ in Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants is comparable to that of wild-types 2 days after egg laying (AEL). Synapse growth during 2-3.5 days AEL of Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants is markedly slower than that in wild-types. From 3.5 to 5 days AEL, the mutant NMJ synapses apparently retract.

The evoked excitatory junction potentials (EJPs) are significantly reduced in Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutant larvae recorded from muscle 6 in the posterior abdominal segment. Miniature EJPs (mEJPs, also known as quantal size) are normal in Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants. Quantal content is significantly reduced in Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants.

(Liu et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neuroanatomy | larval stage phenotype, suppressible by wit^A12/wit[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neuroanatomy | larval stage phenotype, suppressible by tkv⁷/tkv[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neuroanatomy | larval stage phenotype, suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^αTub84B.PL

(Liu et al., 2011)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neurophysiology phenotype, suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^αTub84B.PL

(Liu et al., 2011)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neurophysiology phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2011)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neurophysiology phenotype, suppressible | partially by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2011)

NOT suppressed by

Statement

Reference

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Other

Statement

Reference

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, Rab11^93Bi/Rab11[+] has lethal - all die before end of larval stage phenotype

(Liu et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Acsl⁰⁵⁸⁴⁷/Acsl^KO has neuromuscular junction phenotype, suppressible by wit^A12/wit[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has NMJ bouton | increased number phenotype, suppressible by wit^A12/wit[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 2 neuron phenotype, suppressible by wit^A12/wit[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 3 neuron phenotype, suppressible by wit^A12/wit[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has neuromuscular junction phenotype, suppressible by tkv⁷/tkv[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has NMJ bouton | increased number phenotype, suppressible by tkv⁷/tkv[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 2 neuron phenotype, suppressible by tkv⁷/tkv[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 3 neuron phenotype, suppressible by tkv⁷/tkv[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has neuromuscular junction phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 2 neuron phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 3 neuron phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has NMJ bouton | increased number phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has phenotype, suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^αTub84B.PL

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has bouton phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2011)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has synapse phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2011)

NOT suppressed by

Statement

Reference

Acsl⁰⁵⁸⁴⁷/Acsl^KO has neuromuscular junction phenotype, non-suppressible by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 2 neuron phenotype, non-suppressible by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 3 neuron phenotype, non-suppressible by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has NMJ bouton | increased number phenotype, non-suppressible by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has neuromuscular junction phenotype, non-suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 2 neuron phenotype, non-suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has larval abdominal 3 neuron phenotype, non-suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has NMJ bouton | increased number phenotype, non-suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO has bouton phenotype, non-suppressible by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC}

(Liu et al., 2011)

Other

Statement

Reference

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has NMJ bouton phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has neuromuscular junction phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has larval abdominal 2 neuron phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has larval abdominal 3 neuron phenotype

(Liu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

wit^A12/wit^B11 Acsl⁰⁵⁸⁴⁷/Acsl^KO double mutants show a significant reduction in bouton number at neuromuscular junction 4, similar to wit^A12/wit^B11 mutants.

wit^A12/+ or tkv⁷/+ significantly suppresses synaptic overgrowth of Acsl⁰⁵⁸⁴⁷/Acsl^KO mutants.

Acsl⁰⁵⁸⁴⁷/Acsl^KO Rab11^93Bi/+ animals do not survive to wandering third instar larvae.

(Liu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^elav-C155-, but not Scer\GAL4^Mhc.PW- or Scer\GAL4^repo-mediated expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} rescues the increased total and satellite bouton number in neuromuscular junction 4 of the anterior segments A2 and A3 in Acsl⁰⁵⁸⁴⁷/Acsl^KO animals.

The increase in phospho-Mad levels in the anterior neuromuscular junctions of Acsl⁰⁵⁸⁴⁷/Acsl^KO mutants is suppressed by Scer\GAL4^αTub84B.PL-mediated expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}.

(Liu et al., 2014)

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} in postsynaptic muscles driven by Scer\GAL4^Mhc.PW does not suppress the dystrophic neuromuscular junctions of Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants for bouton number.

Neuronal expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} at 2.5 days after egg laying (AEL) fully suppresses the reduced synaptic area phenotype of Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants.

Neuronal expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} driven by Scer\GAL4^elav.PU restores the bouton number to wild-type levels in Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants.

The neuromuscular junction phenotypes of Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants can be fully suppressed by ubiquitous expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^αTub84B.PL.

The reduced EJPs and quantal content are fully rescued by Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} expressed in either ubiquitously via Scer\GAL4^αTub84B.PL or pan-neuronally via Scer\GAL4^elav.PU. EJPs in animals with Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} expressed pan-neuronally are mildly but significantly higher than that in wild-types.

Postsynaptic expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} driven by Scer\GAL4^Mhc.PW in Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutant background slightly suppressed the EJP phenotype.

(Liu et al., 2011)

Complementation and Rescue Data

Rescued by

Acsl⁰⁵⁸⁴⁷/Acsl^KO is rescued by Scer\GAL4^elav-C155/Acsl^{UAS.715.C.Tag:MYC}

(Liu et al., 2014)

Comments

Scer\GAL4^elav-C155-mediated expression of Acsl^{Scer\UAS.715.C.T:Hsap\MYC} rescues the increased total and satellite bouton number in neuromuscular junction 4 of the anterior segments A2 and A3 in Acsl⁰⁵⁸⁴⁷/Acsl^KO animals.

(Liu et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Acsl^KO

(Jia et al., 2019, Huang et al., 2016, Zhang and Wang, 2010.11.5)

acsl^KO

(Meltzer et al., 2017)

dAcsl^KO

(Liu et al., 2014, Liu et al., 2011, Zhang et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

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