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General Information
Symbol
Dmel\Acsl05847
Species
D. melanogaster
Name
FlyBase ID
FBal0008097
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)05847, dAcsl05847
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{PZ} insertion in intron 1 of the CT25221 transcript of l(2)44DEa, 3089bp downstream of the 5' end of the transcript, 473bp downstream of the end of exon 1. P{PZ} element is inserted in the same orientation as the gene.

Insertion components
P{PZ}Acsl05847
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Neuromuscular junction 4 (NMJ4) terminals look similar across larval abdominal segments A3-A6 in wild type. However, in Acsl05847/AcslKO mutants, NMJs innervating the anterior segment muscles have shorter axons that are overgrown with more satellite boutons, whereas those innervating the posterior segment muscles have longer axons and are dystrophic with relatively few boutons. Acsl8/AcslKO mutants show a similar, but weaker, phenotype.

Acsl05847/AcslKO neuromuscular junction boutons of abdominal segments A2 and A3 display a normal profile of membrane organelles by electron microscopy.

Acsl05847 mutant survive to the pharate adult stage.

In the cross section of the larval segmental nerves of AcslKO/Acsl05847 mutants, there are conspicuous axonal swellings packed with heterogeneous membranous organelles. These aggregates, most often observed in posterior axons, include dark, prelysosomal and multivesicular bodies. In some mutant larvae, autophagosomes are also observed where a cluster of large clear-core vesicles are surrounded by a double-layer membrane. The mutant nerves also display lamellated bodies which resemble autolysosomes. In other cases, clusters of large clear-core vesicles are observed. These membrane aggregates are rarely found in wild-type axons.

AcslKO/Acsl05847 mutants display pronounced dystrophy of larval neuromuscular junction 4 (NMJ4) in the posterior abdominal segments A6 and A7, while the muscle and larval size are comparable to wild-type. The bouton number and the synaptic area are significantly reduced in AcslKO/Acsl05847 mutants compared with wild-type.

The synaptic area of AcslKO/Acsl05847 in AcslKO/Acsl05847 mutants is comparable to that of wild-types 2 days after egg laying (AEL). Synapse growth during 2-3.5 days AEL of AcslKO/Acsl05847 mutants is markedly slower than that in wild-types. From 3.5 to 5 days AEL, the mutant NMJ synapses apparently retract.

The evoked excitatory junction potentials (EJPs) are significantly reduced in AcslKO/Acsl05847 mutant larvae recorded from muscle 6 in the posterior abdominal segment. Miniature EJPs (mEJPs, also known as quantal size) are normal in AcslKO/Acsl05847 mutants. Quantal content is significantly reduced in AcslKO/Acsl05847 mutants.

Neutral lipid accumulation is blocked in Acsl8/Acsl05847 wing discs.

Total triacylglycerol content of Acsl8/Acsl05847 pharates is significantly lower than wild type.

Neuroblast squashes reveal premature separation of sister chromatids during prophase and metaphase, overcondensed chromosomes in metaphase and anaphase, and anaphases with different degrees of abnormalities. Spindles are abnormal, some being of abnormal shape and others failing to connect all the chromosomes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference

Acsl05847/l(2)44DEa[+] is a suppressor of visible | heat sensitive phenotype of peb1

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Acsl05847/Acsl8 has neuromuscular junction phenotype, enhanceable by Dadj1E4/Dad[+]

Acsl05847/Acsl8 has NMJ bouton | increased number phenotype, enhanceable by Dadj1E4/Dad[+]

Acsl05847/Acsl8 has neuromuscular junction phenotype, enhanceable by Rab1193Bi/Rab11[+]

Suppressed by
Statement
Reference

Acsl05847/AcslKO has neuromuscular junction phenotype, suppressible by witA12/wit[+]

Acsl05847/AcslKO has NMJ bouton | increased number phenotype, suppressible by witA12/wit[+]

Acsl05847/AcslKO has A2 neuron phenotype, suppressible by witA12/wit[+]

Acsl05847/AcslKO has A3 neuron phenotype, suppressible by witA12/wit[+]

Acsl05847/AcslKO has neuromuscular junction phenotype, suppressible by tkv7/tkv[+]

Acsl05847/AcslKO has NMJ bouton | increased number phenotype, suppressible by tkv7/tkv[+]

Acsl05847/AcslKO has A2 neuron phenotype, suppressible by tkv7/tkv[+]

Acsl05847/AcslKO has A3 neuron phenotype, suppressible by tkv7/tkv[+]

NOT suppressed by
Suppressor of
Statement
Reference

Acsl05847/l(2)44DEa[+] is a suppressor of eye | heat sensitive phenotype of peb1

Other
Additional Comments
Genetic Interactions
Statement
Reference

witA12/witB11 Acsl05847/AcslKO double mutants show a significant reduction in bouton number at neuromuscular junction 4, similar to witA12/witB11 mutants.

witA12/+ or tkv7/+ significantly suppresses synaptic overgrowth of Acsl05847/AcslKO mutants.

Dadj1E4/+ significantly increases synaptic overgrowth of Acsl05847/Acsl8 mutants.

Acsl05847/AcslKO Rab1193Bi/+ animals do not survive to wandering third instar larvae.

Rab1193Bi/+ and, to a greater extent, Rab1193Bi/Rab1193Bi significantly increases neuromuscular junction overgrowth of Acsl05847/Acsl8 mutants.

Xenogenetic Interactions
Statement
Reference

Scer\GAL4elav-C155-, but not Scer\GAL4Mhc.PW- or Scer\GAL4repo-mediated expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC rescues the increased total and satellite bouton number in neuromuscular junction 4 of the anterior segments A2 and A3 in Acsl05847/AcslKO animals.

The increase in phospho-Mad levels in the anterior neuromuscular junctions of Acsl05847/AcslKO mutants is suppressed by Scer\GAL4αTub84B.PL-mediated expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC.

Expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC in postsynaptic muscles driven by Scer\GAL4Mhc.PW does not suppress the dystrophic neuromuscular junctions of AcslKO/Acsl05847 mutants for bouton number.

Neuronal expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC at 2.5 days after egg laying (AEL) fully suppresses the reduced synaptic area phenotype of AcslKO/Acsl05847 mutants.

Neuronal expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC driven by Scer\GAL4elav.PU restores the bouton number to wild-type levels in AcslKO/Acsl05847 mutants.

The neuromuscular junction phenotypes of AcslKO/Acsl05847 mutants can be fully suppressed by ubiquitous expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC under the control of Scer\GAL4αTub84B.PL.

The reduced EJPs and quantal content are fully rescued by Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC expressed in either ubiquitously via Scer\GAL4αTub84B.PL or pan-neuronally via Scer\GAL4elav.PU. EJPs in animals with Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC expressed pan-neuronally are mildly but significantly higher than that in wild-types.

Postsynaptic expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC driven by Scer\GAL4Mhc.PW in AcslKO/Acsl05847 mutant background slightly suppressed the EJP phenotype.

Expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC or Hsap\ACSL3Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub partially rescues the lethality associated with the Acsl8/Acsl05847 genotype.

Expression of Hsap\ACSL4R570S.Scer\UAS.L.T:Hsap\MYC or Hsap\ACSL4P375L.Scer\UAS.L.T:Hsap\MYC under the control of Scer\GAL4tub fails to rescue the lethality associated with the Acsl8/Acsl05847 genotype.

Expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC, Hsap\ACSL4Scer\UAS.S.T:Hsap\MYC or Hsap\ACSL3Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub restores neutral lipid staining in Acsl8/Acsl05847 wing discs.

Expression of Hsap\ACSL4R570S.Scer\UAS.L.T:Hsap\MYC or Hsap\ACSL4P375L.Scer\UAS.L.T:Hsap\MYC under the control of Scer\GAL4tub fails to restore neutral lipid staining in Acsl8/Acsl05847 wing discs.

Expression of Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC under the control of Scer\GAL4tub restores normal triacylglycerol levels in Acsl8/Acsl05847 wing discs.

Complementation and Rescue Data
Partially rescued by
Comments

Scer\GAL4elav-C155-mediated expression of AcslScer\UAS.715.C.T:Hsap\MYC rescues the increased total and satellite bouton number in neuromuscular junction 4 of the anterior segments A2 and A3 in Acsl05847/AcslKO animals.

Expression of AcslScer\UAS.715.T:Hsap\MYC.C under the control of Scer\GAL4tub partially rescues the lethality associated with the Acsl8/Acsl05847 genotype.

Expression of AcslScer\UAS.715.T:Hsap\MYC.C under the control of Scer\GAL4tub restores neutral lipid staining in Acsl8/Acsl05847 wing discs.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

The P{EP}l(2)44DEaEP2365 insertion is 286bp downstream of the P{PZ}l(2)44DEa05847 insertion.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (13)