Neuromuscular junction 4 (NMJ4) terminals look similar across larval abdominal segments A3-A6 in wild type. However, in Acsl05847/AcslKO mutants, NMJs innervating the anterior segment muscles have shorter axons that are overgrown with more satellite boutons, whereas those innervating the posterior segment muscles have longer axons and are dystrophic with relatively few boutons. Acsl8/AcslKO mutants show a similar, but weaker, phenotype.
Acsl05847/AcslKO neuromuscular junction boutons of abdominal segments A2 and A3 display a normal profile of membrane organelles by electron microscopy.
Acsl05847 mutant survive to the pharate adult stage.
In the cross section of the larval segmental nerves of AcslKO/Acsl05847 mutants, there are conspicuous axonal swellings packed with heterogeneous membranous organelles. These aggregates, most often observed in posterior axons, include dark, prelysosomal and multivesicular bodies. In some mutant larvae, autophagosomes are also observed where a cluster of large clear-core vesicles are surrounded by a double-layer membrane. The mutant nerves also display lamellated bodies which resemble autolysosomes. In other cases, clusters of large clear-core vesicles are observed. These membrane aggregates are rarely found in wild-type axons.
AcslKO/Acsl05847 mutants display pronounced dystrophy of larval neuromuscular junction 4 (NMJ4) in the posterior abdominal segments A6 and A7, while the muscle and larval size are comparable to wild-type. The bouton number and the synaptic area are significantly reduced in AcslKO/Acsl05847 mutants compared with wild-type.
The synaptic area of AcslKO/Acsl05847 in AcslKO/Acsl05847 mutants is comparable to that of wild-types 2 days after egg laying (AEL). Synapse growth during 2-3.5 days AEL of AcslKO/Acsl05847 mutants is markedly slower than that in wild-types. From 3.5 to 5 days AEL, the mutant NMJ synapses apparently retract.
The evoked excitatory junction potentials (EJPs) are significantly reduced in AcslKO/Acsl05847 mutant larvae recorded from muscle 6 in the posterior abdominal segment. Miniature EJPs (mEJPs, also known as quantal size) are normal in AcslKO/Acsl05847 mutants. Quantal content is significantly reduced in AcslKO/Acsl05847 mutants.
Neutral lipid accumulation is blocked in Acsl8/Acsl05847 wing discs.
Total triacylglycerol content of Acsl8/Acsl05847 pharates is significantly lower than wild type.
Neuroblast squashes reveal premature separation of sister chromatids during prophase and metaphase, overcondensed chromosomes in metaphase and anaphase, and anaphases with different degrees of abnormalities. Spindles are abnormal, some being of abnormal shape and others failing to connect all the chromosomes.