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General Information
Symbol
Dmel\spink09905
Species
D. melanogaster
Name
FlyBase ID
FBal0064248
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)k09905, Spin5, k09905
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{lacW} insertion with the spin transcription unit.

Insertion components
P{lacW}spink09905
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Unlike controls, spin10403/spink09905 and spin10403/spinDelta58 transheterozygotes accumulate lamellar/multi-lamellar whorls and lysosomes in the adult brain.

Homozygous larval instar eye imaginal discs show a number of defects in morphology and glial cell migration; in some cases glial cells are found anterior to the morphogenetic furrow and appear to migrate in a broad stream onto the eye imaginal disc, while in others, only a relatively thin stream of of glial cells overshoot anterior to the morphogenetic furrow. Hemizygous eye discs show defects where a duplication of the morphogenetic furrow and the corresponding field of photoreceptor neurons is seen at 90[o] to the normal eye field.

65% of spinEP822/spink09905 stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls. 24% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

77% of spink09905 germ line clone stage 14 egg chambers show persisting nurse cell nuclei. 14% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

Mutation is lethal at the pharate pupal stage. spink09905/+, spin10403/spink09905 and spink09905/Df(2R)Jp4 animals show a significant increase in bouton number at the larval neuromuscular junction (assayed at muscles 6/7 in hemisegment A3) compared to wild type. Quantal content at the neuromuscular junction is decreased by approximately 50% compared to wild type in spin10403/spink09905 animals. Quantal size is unaffected. Only 23% of spin10403/spink09905 animals are adult viable.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

One copy of witA12, tkv7 or sax4 suppresses the increased bouton number seen at the neuromuscular junction in spin10403/spink09905 animals. spin10403/spink09905 animals which are also mutant for witA12/witB11, tkv7/tkvk16713 or sax4/Df(2R)cn7969 show a further decrease in bouton number, either to wild type or below wild type levels.

Xenogenetic Interactions
Statement
Reference

spink09905 dominantly suppresses the rough eye phenotype caused by expression of Zzzz\CTGi480.Scer\UAS.cGa under the control of Scer\GAL4sev.PU.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Complements: l(2)0507005070. Complements: spin10403. Complements: Rho1k02107b. Complements: bnchk02511. Complements: l(2)k15617k15617.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (12)