FlyBase Allele Report: Dmel\CDase[slab-2]

General Information

Symbol

Dmel\CDase^slab-2

Species

D. melanogaster

Name

FlyBase ID

FBal0213207

Feature type

allele

Associated gene

Dmel\CDase

Associated Insertion(s)

P{lacW}CDase^slab-2

Carried in Construct

Also Known As

slab²

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Rohrbough et al., 2004)

Progenitor genotype

P{lacW}l(3)j8B9^j8B9

(Rohrbough et al., 2004)

Associated Insertion(s)

P{lacW}CDase^slab-2

Cytology

Description

Mobilization of the P{lacW} element, resulting in an intragenic deletion of 855 bases that removes parts of exons 4 and 5 of the CDase gene. The original P{lacW}l(3)j8B9^j8B9 insertion site is retained and there is a second insertion within CDase.

(Rohrbough et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates primary autosomal dominant microcephaly 18

modeled by bchs⁵⁸

(Hebbar et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology (with CDase^slab-1)

(Rohrbough et al., 2004)

lethal - all die during embryonic stage

(Rohrbough et al., 2004)

lethal - all die during embryonic stage (with CDase^slab-1)

(Rohrbough et al., 2004)

lethal - all die during embryonic stage (with Df(3R)slab-3)

(Rohrbough et al., 2004)

some die during embryonic stage

(Rohrbough et al., 2004)

some die during embryonic stage (with CDase^slab-1)

(Rohrbough et al., 2004)

some die during embryonic stage (with Df(3R)slab-3)

(Rohrbough et al., 2004)

Phenotype Manifest In

synaptic vesicle (with CDase^slab-1)

(Rohrbough et al., 2004)

Detailed Description

Statement

Reference

CDase^slab-2 embryos appear anatomically and morphologically normal. Although mutants move spontaneously and in response before hatching, movements are less vigorous than in controls. A minority briefly generate peristaltic body contractions sufficient to emerge partly or completely from the egg case. Physiological assays show that hypertonic saline-evoked synaptic vesicle fusion is significantly impaired in CDase^slab-1/CDase^slab-2 embryos.

(Rohrbough et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

CDase[+]/CDase^slab-2 is a suppressor | partially of increased mortality during development phenotype of spin^k09905/spin¹⁰⁴⁰³

(Hebbar et al., 2017)

CDase[+]/CDase^slab-2 is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Df(2L)cl7/bchs⁵⁸