65% of spin^EP822/spin^k09905 stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls. 24% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

65% of spin^EP822/spin¹⁰⁴⁰³ stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls. 52% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

73% of spin^EP822 germ line clone stage 14 egg chambers show persisting nurse cell nuclei. 27% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

Mutation is lethal at the pharate pupal stage. Heterozygotes, homozygotes and spin^EP822/Df(2R)Jp4 hemizygotes show a significant increase in bouton number at the larval neuromuscular junction (assayed at muscles 6/7 in hemisegment A3) compared to wild type.

The abdominal part of the CNS is abnormally long in homozygous adults compared to heterozygous or wild-type adults. This long-abdominal-ganglion phenotype becomes evident between 24 and 48 hours after puparium formation (APF). At 48 hours APF the abdominal ganglia are distorted in shape and the shortening of the tail part (which has occurred in wild-type animals) is incomplete. The mutant CNS does not undergo the transient peak in programmed cell death at 6 hours APF which is seen in wild-type animals.

The abdominal ganglion of mutant adult flies is longer than normal. The number of apoptotic cells in the ventral nerve cord after eclosion is the same in homozygous and control animals. In contrast, the profile of programmed cell death in the ventral nerve cord during the pupal stage is significantly different in homozygous animals compared to controls; at 6 hours after puparium formation the number of cells undergoing apoptosis in the homozygotes is significantly lower than in control animals. Dorsal appendages are well formed in stage 14 egg chambers of mutant females, but the nurse cell nuclei are still present (in contrast to controls, where the nurse cell nuclei have degenerated by this stage) and some earlier stage egg chambers are degenerated.