FlyBase Allele Report: Dmel\tkv[k16713]

General Information

Symbol

Dmel\tkv^k16713

Species

D. melanogaster

Name

FlyBase ID

FBal0063746

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

P{lacW}tkv^k16713

Carried in Construct

Also Known As

tkv-lacZ, tkv¹⁶⁷¹³, tkv^k16713-lacZ

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(George and Terracol, 1997)

Mutagen

P-element activity

(George and Terracol, 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}tkv^k16713

Cytology

Description

P{lacW} insertion in second intron.

(Misra, 2000.8.25)

P{lacW} insertion maps 600bp upstream of P{PZ} insertion of tkv⁰⁴⁴¹⁵ within the tkv-2 cDNA second intron.

(George and Terracol, 1997)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:5,237,595..5,237,595 [+]

(West et al., 2015, Dworkin and Gibson, 2006, Misra, 2000.8.25, George and Terracol, 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Angelman syndrome

modeled by Ube3a³⁵

(Li et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

bouton (with tkv⁷)

(Sweeney and Davis, 2002)

embryonic/larval neuromuscular junction (with tkv⁷)

(Zhao et al., 2015)

neuromuscular junction (with tkv⁷)

(Sweeney and Davis, 2002)

NMJ bouton | third instar larval stage (with tkv⁸)

(Li et al., 2016)

wing

(Dworkin and Gibson, 2006)

Detailed Description

Statement

Reference

tkv⁸/tkv^k16713 third instar larvae have a significant decrease in total boutons (and satellite boutons) at the neuromuscular junction, compared to wild type.

(Li et al., 2016)

The neuromuscular junction synapses of homozygous tkv⁷/tkv^k16713 mutants are undergrown but the total number of boutons is similar to heterozygous controls.

(Zhao et al., 2015)

Heterozygotes show a direct effect on the shape of the wing.

(Dworkin and Gibson, 2006)

tkv^k16713/tkv⁷ larvae show a significant decrease in bouton number at the neuromuscular junction.

(Sweeney and Davis, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

tkv⁸/tkv^k16713 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Ube3a³⁵/Ube3a³⁵

(Li et al., 2016)

Suppressor of

Statement

Reference

tkv⁸/tkv^k16713 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Ube3a³⁵

(Li et al., 2016)

tkv^k16713/tkv[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

tkv^k16713/tkv⁷ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

tkv^k16713/tkv⁷ is a suppressor of abnormal neuroanatomy phenotype of spict^mut

(Wang et al., 2007)

tkv^k16713/tkv[+] is a suppressor of lethal | recessive | partially | embryonic stage phenotype of peb³⁰⁸

(Wilk et al., 2004)

tkv^k16713/tkv[+] is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

tkv^k16713 is a suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

tkv^k16713/tkv⁷ is a suppressor of abnormal neuroanatomy phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

Other

Statement

Reference

Dys^E6, tkv^k16713/tkv[+] has visible phenotype

(Kucherenko et al., 2008)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

tkv⁸/tkv^k16713 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Ube3a³⁵/Ube3a³⁵

(Li et al., 2016)

NOT Enhancer of

Statement

Reference

tkv^k16713/tkv[+] is a non-enhancer of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

Suppressor of

Statement

Reference

tkv⁸/tkv^k16713 is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Ube3a³⁵

(Li et al., 2016)

tkv^k16713/tkv[+] is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

tkv^k16713/tkv[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

tkv^k16713/tkv⁷ is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

tkv^k16713/tkv⁷ is a suppressor of NMJ bouton | third instar larval stage phenotype of Rab8¹/Rab8^B229

(West et al., 2015)

tkv^k16713/tkv⁷ is a suppressor of embryonic/larval neuromuscular junction phenotype of spict^mut

(Wang et al., 2007)

tkv^k16713/tkv[+] is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

tkv^k16713 is a suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

tkv^k16713/tkv⁷ is a suppressor of bouton | increased number phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

tkv^k16713/tkv⁷ is a suppressor of neuromuscular junction phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

NOT Suppressor of

Statement

Reference

tkv^k16713/tkv[+] is a non-suppressor of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

Other

Statement

Reference

Dys^E6, tkv^k16713/tkv[+] has posterior crossvein phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

tkv⁸/tkv^k16713;Ube3a³⁵/Ube3a³⁵ third instar larvae have a significant decrease in total boutons (and satellite boutons) at the neuromuscular junction, compared to wild type (similar to tkv⁸/tkv^k16713, suppressing the increase seen in Ube3a³⁵/Ube3a³⁵ mutants).

(Li et al., 2016)

When present in the genetic background, heterozygous tkv^k16713 reduces the synaptic overgrowth in Rab8¹/Rab8^B229 mutants.

When present in the genetic background, trans-heterozygous tkv⁷/tkv^k16713 reduces the synaptic overgrowth in Rab8¹/Rab8^B229 mutants.

(West et al., 2015)

One copy of tkv^k16713 in a Dys^E6/+ background results in posterior crossvein defects.

(Kucherenko et al., 2008)

spict^mut neuromuscular junction overgrowth phenotypes are fully suppressed in tkv⁷/tkv^k16713 mutants. The synaptic undergrowth phenotypes in larvae homozygous for spict^mut in a tkv⁷/tkv^k16713 background are indistinguishable from that of tkv⁷/tkv^k16713 mutants alone.

(Wang et al., 2007)

spin¹⁰⁴⁰³/spin^k09905 animals which are also mutant for tkv⁷/tkv^k16713 show a decrease in bouton number.

(Sweeney and Davis, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

tkv⁰⁴⁴¹⁵

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11191

y¹ w^67c23; P{lacW}tkv^k16713/CyO

Kyoto

111360

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}tkv^k16713 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Complements: cype⁰³⁷⁷¹. Complements: l(2)k01302^k01302. Complements: vri^k05901.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Tkv^K16713

(West et al., 2015)

k16713

(Dworkin et al., 2011)

k19713

(Dworkin and Gibson, 2006)

l(2)k16713

(Misra, 2000.8.25)

tkv¹⁶¹⁷³

(Bach et al., 2003)

tkv¹⁶⁷¹³

(Wang et al., 2007, Sweeney and Davis, 2002)

tkv^K16713

(Li et al., 2016)

tkv^K19713

(Dworkin and Gibson., 2006)

tkv^K

(Li et al., 2016)

tkv^k16713

(Li et al., 2020, Pinto-Teixeira et al., 2018, Zhao et al., 2015, Huang et al., 2011, Quijano et al., 2011, Kucherenko et al., 2008, Cordero et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

Open Close