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General Information
Symbol
Dmel\Rab8B229
Species
D. melanogaster
Name
FlyBase ID
FBal0159939
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    PBac{5HPw+}Rab8B229
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Rab81/Rab8B229 mutants show pharate lethality.

    All the tested trans-heterozygous allelic combinations of Rab8B229 mutants display synaptic overgrowth, characterized by significant increase in synaptic bouton number of up to 100% relative to wild-type.

    In addition to elevated bouton number, Rab81/Rab8B229 mutants also have a significant increase in neuromuscular junction length, branching, and satellite bouton number, with reduction in synaptic bouton size.

    Postsynaptic expression of Rab8Scer\UAS.cWa under the control of Scer\GAL4Mhc.PW rescues the increased synaptic bouton number phenotype at muscle 6/7, but not synapse length at neuromuscular junctions in Rab81/Rab8B229 mutants.

    Synaptic bouton number or length in muscle 4 is not rescued by muscle expression of Rab8Scer\UAS.cWa.

    Rab81/Rab8B229 mutants display a clear reduction in both fast and slow recycling endosomes at both the NMJ and in motor neuron cell bodies in the larval ventral nerve cord. Upon ultrastructural analysis of mutant synapses, accumulation of large endosome-like, intermediary structures within synaptic boutons are observed. The observed structures, however, are dissimilar and unlikely synaptic vesicles as they are not released, because there is no increase in quantal size during electrophysiological analysis. On the contrary, Rab81/Rab8B229 mutants show decreased quantal size compared with wild-type. These structures are similar to autophagosomal intermediates and they are never observed in wild-type animals.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Suppressed by
    Statement
    Reference

    Rab81/Rab8B229 has NMJ bouton | third instar larval stage phenotype, suppressible by tkv7/tkv[+]

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    When present in the genetic background, heterozygous tkv7 reduces the synaptic overgrowth in Rab81/Rab8B229 mutants.

    When present in the genetic background, heterozygous tkvk16713 reduces the synaptic overgrowth in Rab81/Rab8B229 mutants.

    When present in the genetic background, trans-heterozygous tkv7/tkvk16713 reduces the synaptic overgrowth in Rab81/Rab8B229 mutants.

    Neuronal expression of kayFbz.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 is sufficient to completely alleviate the synaptic overgrowth in Rab81/Rab8B229 mutants.

    Neuronal expression of kayFbz.Scer\UAS under the control of Scer\GAL4nSyb.PS is sufficient to completely alleviate the synaptic overgrowth in Rab81/Rab8B229 mutants.

    Introducing POSHEP1206 into a Rab81/Rab8B229 mutant background alleviates the synaptic overgrowth phenotype.

    Introducing POSHEP2248 into a Rab81/Rab8B229 mutant background alleviates the synaptic overgrowth phenotype.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    Expression of Rab8Scer\UAS.cWa under the control of Scer\GAL4Act5C.PI and the presynaptic driver Scer\GAL4nSyb.PS is sufficient to completely rescue the increased bouton number at muscles 6/7 Rab81/Rab81 mutants.

    Postsynaptic expression of Rab8Scer\UAS.cWa under the control of Scer\GAL4Mhc.PW rescues the increased synaptic bouton number phenotype at muscle 6/7, but not synapse length at neuromuscular junctions in Rab81/Rab8B229 mutants.

    Synaptic bouton number or length in muscle 4 is not rescued by muscle expression of Rab8Scer\UAS.cWa.

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    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Symbol Synonym
    Rab8B229
    Name Synonyms
    Secondary FlyBase IDs
      References (3)