FlyBase Allele Report: Dmel\wit[HA3]

General Information

Symbol

Dmel\wit^HA3

Species

D. melanogaster

Name

FlyBase ID

FBal0133965

Feature type

allele

Associated gene

Dmel\wit

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Aberle et al., 2002)

Mutagen

ethyl methanesulfonate

(Aberle et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: W491term.

(Aberle et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:4,066,546..4,066,546 [-]

Nucleotide change:

G4066546A

Amino acid change:

W491term | wit-PA; W491term | wit-PB

Reported amino acid change:

W491term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Aberle et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with wit^A12)

(Ball et al., 2010)

abnormal neurophysiology | third instar larval stage (with wit^A12)

(Ball et al., 2010)

abnormal size | third instar larval stage (with wit^A12)

(Ball et al., 2010)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage (with wit^A12)

(Ball et al., 2010)

NMJ bouton | third instar larval stage (with wit^A12)

(Ball et al., 2010)

Detailed Description

Statement

Reference

wit^A12/wit^HA3 third instar larvae exhibit neuromuscular junction overgrowth and a reduced number of boutons per muscle surface area in muscle 4 compared to controls.

(Ball et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

wit^HA3/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

wit^B11/wit^HA3 has abnormal neuroanatomy phenotype, suppressible by tkv::wit^{tkv-EC.wit-IC.Ubi-p63E}/tkv::wit^{wit-EC.tkv-IC.Ubi-p63E}

(McCabe et al., 2004)

NOT suppressed by

Statement

Reference

wit^HA3/wit^A12 has abnormal size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.UAS

(Ball et al., 2010)

wit^HA3/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.UAS

(Ball et al., 2010)

wit^HA3/wit^A12 has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.cLa

(Ball et al., 2010)

wit^HA3/wit^A12 has abnormal size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

wit^HA3/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

Suppressor of

Statement

Reference

wit^HA3/wit^A12 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a suppressor of abnormal size | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

NOT Suppressor of

Statement

Reference

wit^HA3/wit^A12 is a non-suppressor of abnormal size | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Other

Statement

Reference

Mad¹, wit^HA3/wit[+] has abnormal neuroanatomy | dominant phenotype

(McCabe et al., 2004)

tkv⁸, wit^HA3/wit[+] has abnormal neuroanatomy | dominant phenotype

(McCabe et al., 2004)

Mad[+]/Mad¹, wit^HA3 has abnormal neuroanatomy phenotype

(McCabe et al., 2004)

Med[+]/Med^C246, wit^HA3 has abnormal neuroanatomy phenotype

(McCabe et al., 2004)

sax⁴/sax[+], wit^HA3 has abnormal neuroanatomy phenotype

(McCabe et al., 2004)

tkv⁸/tkv[+], wit^HA3 has abnormal neuroanatomy phenotype

(McCabe et al., 2004)

Med^C246, wit^HA3/wit[+] has abnormal neuroanatomy | dominant phenotype

(McCabe et al., 2004)

sax⁴, wit^HA3/wit[+] has abnormal neuroanatomy | dominant phenotype

(McCabe et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

wit^HA3/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

wit^HA3/wit^A12 has NMJ bouton | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

wit^B11/wit^HA3 has neuromuscular junction phenotype, suppressible by tkv::wit^{tkv-EC.wit-IC.Ubi-p63E}/tkv::wit^{wit-EC.tkv-IC.Ubi-p63E}

(McCabe et al., 2004)

NOT suppressed by

Statement

Reference

wit^HA3/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

wit^HA3/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

wit^HA3/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.UAS

(Ball et al., 2010)

wit^HA3/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.UAS

(Ball et al., 2010)

Suppressor of

Statement

Reference

wit^HA3/wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

NOT Suppressor of

Statement

Reference

wit^HA3/wit^A12 is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Other

Statement

Reference

Mad¹, wit^HA3/wit[+] has bouton phenotype

(McCabe et al., 2004)

tkv⁸, wit^HA3/wit[+] has neuromuscular junction phenotype

(McCabe et al., 2004)

Mad¹, wit^HA3 has bouton phenotype

(McCabe et al., 2004)

Mad¹, wit^HA3 has neuromuscular junction phenotype

(McCabe et al., 2004)

Med^C246, wit^HA3 has bouton phenotype

(McCabe et al., 2004)

Med^C246, wit^HA3 has neuromuscular junction phenotype

(McCabe et al., 2004)

Mad¹, wit^HA3/wit[+] has neuromuscular junction phenotype

(McCabe et al., 2004)

sax⁴, wit^HA3 has bouton phenotype

(McCabe et al., 2004)

sax⁴, wit^HA3 has neuromuscular junction phenotype

(McCabe et al., 2004)

tkv⁸, wit^HA3 has bouton phenotype

(McCabe et al., 2004)

Med^C246, wit^HA3/wit[+] has bouton phenotype

(McCabe et al., 2004)

tkv⁸, wit^HA3 has neuromuscular junction phenotype

(McCabe et al., 2004)

Med^C246, wit^HA3/wit[+] has neuromuscular junction phenotype

(McCabe et al., 2004)

sax⁴, wit^HA3/wit[+] has bouton phenotype

(McCabe et al., 2004)

sax⁴, wit^HA3/wit[+] has neuromuscular junction phenotype

(McCabe et al., 2004)

tkv⁸, wit^HA3/wit[+] has bouton phenotype

(McCabe et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wit^A12/wit^HA3 suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^{Scer\UAS.T:Hsap\MYC} is expressed in motor neurons under the control of Scer\GAL4^BG380, instead producing a reduction in bouton number similar to the phenotype seen in wit^A12/wit^HA3 third instar larvae.

Expression of wit^A12/wit^HA3 fails to suppress the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^V12.Scer\UAS is expressed in motor neurons under the control of Scer\GAL4^BG380.

wit^A12/wit^HA3 suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1^Scer\UAS.cLa is expressed in motor neurons under the control of Scer\GAL4^BG380, with third instar larvae instead displaying the reduction in neurotransmitter release phenotype seen in wit^A12/wit^HA3 mutants.

Expression of trio^Scer\UAS.cBa in motor neurons under the control of Scer\GAL4^BG380 partially suppresses the reduced bouton number phenotype seen in wit^A12/wit^HA3 larval neuromuscular junctions.

(Ball et al., 2010)

The number of synaptic boutons/muscle surface area at muscle 6/7 in Med^C246/wit^HA3 double heterozygotes is 91.3 +/- 2.7 % of wild type. The number of synaptic boutons/muscle surface area at muscle 6/7 in Mad¹/wit^HA3 double heterozygotes is 95.9 +/- 2.2 % of wild type. The number of synaptic boutons/muscle surface area at muscle 6/7 in sax⁴/wit^HA3 double heterozygotes is 85.2 +/- 3.6 % of wild type. The number of synaptic boutons/muscle surface area at muscle 6/7 in tkv⁸/wit^HA3 double heterozygotes is 88.8 +/- 2.4 % of wild type. Co-expression of tkv::wit^{wit-EC.tkv-IC.Ubi-p63E} and tkv::wit^{tkv-EC.wit-IC.Ubi-p63E} rescues the neuromuscular junction size in wit^HA3/wit^B11 animals.

(McCabe et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

wit^HA3

(Ball et al., 2010, Xiong et al., 2010, Collins et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (5)

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