FlyBase Allele Report: Dmel\Rac1[UAS.Tag:MYC]

General Information

Symbol

Dmel\Rac1^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0135833

Feature type

allele

Associated gene

Dmel\Rac1

Associated Insertion(s)

Carried in Construct

P{UAS-Rac1.Myc}

Also Known As

UAS-Rac1, UAS-Rac1-Myc

Key Links

Transgenic product class

wild_type

(Ng et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ng et al., 2002)

Mutagen

in vitro construct

(Ng et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Rac1.Myc}

Cytology

Description

UAS regulatory sequences drive expression of Rac1 tagged with Tag:MYC.

(Ng et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rac1

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^BG380

(Ball et al., 2010)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^{elav.Switch.PO}

(Ball et al., 2010)

abnormal size | third instar larval stage, with Scer\GAL4^BG380

(Ball et al., 2010)

abnormal size | third instar larval stage, with Scer\GAL4^{elav.Switch.PO}

(Ball et al., 2010)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

dendritic arborizing neuron, with Scer\GAL4^109(2)80

(Lee et al., 2003)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^BG380

(Ball et al., 2010)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^{elav.Switch.PO}

(Ball et al., 2010)

NMJ bouton | third instar larval stage, with Scer\GAL4^BG380

(Ball et al., 2010)

Detailed Description

Statement

Reference

Expression of Rac1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{elav.Switch.PO} during the third instar larval stage is sufficient to cause larval neuromuscular junction overgrowth.

Expression of Rac1^{Scer\UAS.T:Hsap\MYC} under the control of the neuronal driver Scer\GAL4^BG380 leads to neuromuscular junction overgrowth during larval development. Bouton number per muscle area at muscle 4 is increased compared to controls.

(Ball et al., 2010)

Expression of Rac1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107 results in axon growth and guidance defects in the mushroom body.

(Ng and Luo, 2004)

Rac1^{Scer\UAS.T:Hsap\MYC} when driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} has no effect on transepithelial migration of germ cells in the embryo.

(Kunwar et al., 2003)

When Rac1^{Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^109(2)80, increased dendritic branching is seen in the dendritic arborising (DA) neurons.

(Lee et al., 2003)

Expression of Rac1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107 does not disrupt mushroom body axon patterning in a wild-type background.

(Ng et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rac1^UAS.Tag:MYC, Scer\GAL4^ey-OK107 has abnormal neuroanatomy phenotype, enhanceable by LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Suppressed by

Statement

Reference

Rac1^UAS.Tag:MYC, Scer\GAL4^BG380 has abnormal size | third instar larval stage phenotype, suppressible by Mad^k00237/Mad¹

(Ball et al., 2010)

Rac1^UAS.Tag:MYC, Scer\GAL4^{elav.Switch.PO} has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Mad^k00237/Mad¹

(Ball et al., 2010)

Rac1^UAS.Tag:MYC, Scer\GAL4^BG380 has abnormal size | third instar larval stage phenotype, suppressible by wit^HA3/wit^A12

(Ball et al., 2010)

Rac1^UAS.Tag:MYC, Scer\GAL4^BG380 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wit^HA3/wit^A12

(Ball et al., 2010)

Enhancer of

Statement

Reference

Rac1^UAS.Tag:MYC, Scer\GAL4^ey-OK107 is an enhancer of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^BG380/Rac1^UAS.Tag:MYC is a non-suppressor of abnormal size | third instar larval stage phenotype of Mad^k00237/Mad¹

(Ball et al., 2010)

Scer\GAL4^BG380/Rac1^UAS.Tag:MYC is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Mad^k00237/Mad¹

(Ball et al., 2010)

Scer\GAL4^BG380/Rac1^UAS.Tag:MYC is a non-suppressor of abnormal size | third instar larval stage phenotype of wit^HA3/wit^A12

(Ball et al., 2010)

Scer\GAL4^BG380/Rac1^UAS.Tag:MYC is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of wit^HA3/wit^A12

(Ball et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rac1^UAS.Tag:MYC, Scer\GAL4^ey-OK107 has adult mushroom body phenotype, enhanceable by LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Suppressed by

Statement

Reference

Rac1^UAS.Tag:MYC, Scer\GAL4^BG380 has NMJ bouton | third instar larval stage phenotype, suppressible by Mad^k00237/Mad¹

(Ball et al., 2010)

Rac1^UAS.Tag:MYC, Scer\GAL4^BG380 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by wit^HA3/wit^A12

(Ball et al., 2010)

Rac1^UAS.Tag:MYC, Scer\GAL4^BG380 has NMJ bouton | third instar larval stage phenotype, suppressible by wit^HA3/wit^A12

(Ball et al., 2010)

Rac1^UAS.Tag:MYC, Scer\GAL4^BG380 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Mad^k00237/Mad¹

(Ball et al., 2010)

Enhancer of

Statement

Reference

Rac1^UAS.Tag:MYC, Scer\GAL4^ey-OK107 is an enhancer of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Suppressor of

Statement

Reference

Rac1^UAS.Tag:MYC, Scer\GAL4^109(2)80 is a suppressor | partially of dendritic arborizing neuron phenotype of Fmr1^UAS.cWa, Scer\GAL4^109(2)80

(Lee et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^BG380/Rac1^UAS.Tag:MYC is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Mad^k00237/Mad¹

(Ball et al., 2010)

Scer\GAL4^BG380/Rac1^UAS.Tag:MYC is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Mad^k00237/Mad¹

(Ball et al., 2010)

Scer\GAL4^BG380/Rac1^UAS.Tag:MYC is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of wit^HA3/wit^A12

(Ball et al., 2010)

Scer\GAL4^BG380/Rac1^UAS.Tag:MYC is a non-suppressor of NMJ bouton | third instar larval stage phenotype of wit^HA3/wit^A12

(Ball et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Mad¹/Mad^k00237 suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^{Scer\UAS.T:Hsap\MYC} is expressed in motor neurons under the control of Scer\GAL4^BG380, instead producing a reduction in bouton number similar to the phenotype seen in Mad¹/Mad^k00237 third instar larvae.

Expression of wit^A12/wit^HA3 suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^{Scer\UAS.T:Hsap\MYC} is expressed in motor neurons under the control of Scer\GAL4^BG380, instead producing a reduction in bouton number similar to the phenotype seen in wit^A12/wit^HA3 third instar larvae.

(Ball et al., 2010)

Mushroom body axon growth defects in single-cell Rac1^J11 Rac2^Δ Mtl^Δ γ neuron clones are largely rescued by expression of Rac1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107. 81% of axons in Rac1^J11 Rac2^Δ mushroom body clones show mutant phenotypes, predominantly guidance (55%) and branching (24%) defects. Expression of Rac1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107 markedly rescues these defects.

(Ng et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6680

P{UAS-Rac1.Myc}2.4, y¹ w^*; TM3, Sb¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rac1^{Scer\UAS.T:Hsap\MYC}

Rac1^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (7)

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