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General Information
Symbol
Dmel\Madk00237
Species
D. melanogaster
Name
FlyBase ID
FBal0064630
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Mad237, k00237
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{lacW}Madk00237
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mad12/Madk00237 transheterozygotes show a significant reduction in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to controls.

Madk00237 mutants exhibit undergrown synapses with few boutons on neuromuscular junction 4.

Mad1/Madk00237 third instar larvae show a reduced number of boutons per muscle surface area in muscle 4 compared to controls.

Homozygous Madk00237 third instar larvae show a reduced number of boutons per muscle surface area in muscle 4 compared to controls.

The number of boutons per muscle surface area is reduced at the neuromuscular junction in Madk00237 third instar larvae.

Homozygous third instar larvae show a severe reduction in excitatory junctional current amplitude and in quantal content at the neuromuscular junction compared to wild type.

Heterozygotes show a direct effect on the shape of the wing.

Madk00237/Madapg larvae transferred to 29oC at the equivalent of 48 hours, 72 hours, 84 hours or 96 hours after egg laying develop at the same rate as wild-type siblings.

Removal of Mad function before 72 hours after egg laying in Madapg/Madk00237 flies produces truncations of the proximal-distal axis in the legs, whereas removal of Mad function from 84 hours after egg laying until the end of development results in a normal proximal-distal organisation in the leg despite dorsal patterning defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The decreased number of synaptic boutons at the third instar larval neuromuscular of Mad12/Madk00237 transheterozygotes is not suppressed or enhanced by either Nrx-1273 or Nlg1Δ46 homozygosity.

One copy of Madk00237 suppresses the increase in total bouton number seen in S6KL140 mutants.

Rab1193Bi, Madk00237 double mutants exhibit undergrown synapses with few boutons on neuromuscular junction 4 that are indistinguishable from Madk00237 single mutants.

Expression of Mad1/Madk00237 suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1Scer\UAS.T:Hsap\MYC is expressed in motor neurons under the control of Scer\GAL4BG380, instead producing a reduction in bouton number similar to the phenotype seen in Mad1/Madk00237 third instar larvae.

Expression of Mad1/Madk00237 fails to suppress the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1V12.Scer\UAS is expressed in motor neurons under the control of Scer\GAL4BG380.

trioS137203 does not enhance the reduction in bouton number phenotype seen in Madk00237 larval neuromuscular junctions.

Expression of trioScer\UAS.cBa in motor neurons under the control of Scer\GAL4BG380 partially suppresses the reduced bouton number phenotype seen in Mad1/Madk00237 larval neuromuscular junctions.

Madk00237/+ ; nmoP1/+ and Madk00237/+ ; Df(3L)Exel6279/+ double heterozygous third instar larvae show a significant reduction in the number of boutons per muscle surface area at the neuromuscular junction, in contrast to any of the single heterozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Madhs.PK using heat shock partially rescues the lethality of Madk00237 mutants, with the rescue efficiency being increased with more frequent heat shocks.

Expression of MadT312V.hs using heat shock partially rescues the lethality of Madk00237 mutants, with the rescue efficiency being increased with more frequent heat shocks. The degree of rescue with MadT312V.hs is more effective than that seen with Madhs.PK under the same conditions.

Neuronal expression of MadScer\UAS.T:Hsap\MYC under the control of Scer\GAL4unspecified rescues the reduction in the number of boutons per muscle surface area that is seen at the neuromuscular junction in Madk00237 third instar larvae.

Neuronal expression of MadS25A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4unspecified partially rescues the reduction in the number of boutons per muscle surface area that is seen at the neuromuscular junction in Madk00237 third instar larvae.

Expression of either MadScer\UAS.T:Hsap\MYC or MadS25A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4BG380 rescues the electrophysiological defects seen at the neuromuscular junction of Madk00237 third instar larvae.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (22)