Expression of NPF^UAS.cWa under the control of Scer\GAL4^NPF.1 in males does not affect courtship towards males and significantly increases aggressive lunging towards males, compared to controls.

The expression of NPF^UAS.cWa under the control of Scer\GAL4^NP1624 leads to virgin females showing an increase in the number of germline stem cells in the germarium, as compared to controls.

NPF^sk1/Df(3R)ED10642 transheterozygous females fail to show the expected mating-induced increase in germline stem cells in the germarium, as compared to controls; the expression of NPF^UAS.cWa under the control of Scer\GAL4^Tk.PS rescues the germline stem cell number in mated females, but also leads to an abnormal increase in germline stem cells in virgin females.

Over-expression of NPF^Scer\UAS.cWa in Scer\GAL4^NPF.1 neurons does not significantly affect total sleep amount compared to genetic controls.

When npf^Scer\UAS.cWa is driven by Scer\GAL4^386Y or Scer\GAL4^npf.1 mutant animals are highly sensitive to alcohol. When npf^Scer\UAS.cWa is driven by Scer\GAL4^npf.1 mutant animals show a normal response to ethyl ether exposure.

Larvae expressing npf^Scer\UAS.cWa under the control of Scer\GAL4^386Y and control larvae develop similarly during the feeding phase and have similar body sizes at 98 hours after egg laying. The path length of larvae expressing npf^Scer\UAS.cWa under the control of Scer\GAL4^386Y crawling on water-agarose paste is similar to that of wild-type larvae. Older third instar larvae (100 hours after egg laying) expressing npf^Scer\UAS.cWa under the control of Scer\GAL4^386Y which are placed on a glucose-agar plate coated with a thin layer of yeast paste browse evenly across the assay plate (the plate is examined after 45 minutes). This behaviour is not typical of older wild-type larvae, which tend to migrate to the periphery of the plate and form clumps of larvae at the periphery, but is instead typical of young wild-type third instar larvae. Older third instar larvae (100 hours after egg laying) expressing npf^Scer\UAS.cWa under the control of Scer\GAL4^386Y placed on an apple juice-agar plate show no social interaction and completely fail to burrow into the agar, even after 1 hour. This is in contrast to control larvae, which show social burrowing and penetrate the agar in less than 30 minutes. Larvae expressing npf^Scer\UAS.cWa under the control of Scer\GAL4^386Y do not stop feeding until 12-24 hours later than controls and the mutant larvae are delayed in forming puparia by about the same amount of time. In the absence of food, well-fed early third instar larvae expressing npf^Scer\UAS.cWa under the control of Scer\GAL4^386Y and control larvae develop into puparia at the same rate. Non-feeding (120 hours after egg laying) larvae expressing npf^Scer\UAS.cWa under the control of Scer\GAL4^386Y show neither food aversion nor social burrowing when placed on an apple juice-agar plate. Most of these larvae form puparia on the moist food-containing agar surface, in contrast to control larvae, which mostly form puparia on the dry plastic surface of the plate.