FlyBase Allele Report: Scer\GAL4[Tk.PS]

General Information

Symbol

Scer\GAL4^Tk.PS

Species

S. cerevisiae

Name

FlyBase ID

FBal0301645

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Tk-GAL4.PS}

Also Known As

TKg-Gal4, TK-Gal4, Tk-gut-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Song et al., 2014)

Progenitor genotype

Carried in construct

P{Tk-GAL4.PS}

Cytology

Description

Tk promoter sequences drive expression of GAL4 protein coding sequences, specifically in the gut enteroendocrine cells and not Tk neurons.

(Song et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval enteroendocrine cell

(Song et al., 2017)

adult stage

enteroendocrine cell

(Song et al., 2014)

adult enteroendocrine cell

(Ameku et al., 2018)

adult brain | restricted

(Ameku et al., 2018)

adult ventral nerve cord | restricted

(Ameku et al., 2018)

Additional Information

Statement

Reference

ScerGAL4^Tk.PS drives expression in NPF positive adult enteroendocrine cells as well as cells in the brain and thoracico-abdominal ganglion, a subset of which are also positive for NPF.

(Ameku et al., 2018)

ScerGAL4^Tkg.PS drives expression only in Tachykin-secreting enteroendocrine cells in the midgut, and not in Tachykinin-secreting neurons in the brain.

(Song et al., 2014)

Marker for

enteroendocrine cell

(Song et al., 2014)

Reflects expression of

(Song et al., 2014)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | adult stage, with GulpR^HMS05678

(Barraza et al., 2025)

abnormal cell number | adult stage, with GulpR^JF03036

(Barraza et al., 2025)

abnormal heat stress response | larval stage, with Phae1^KK106428

(Matsumura et al., 2025)

abnormal immune response | adult stage, with AcCoAS^HMS02314

(Jugder et al., 2021)

abnormal immune response | adult stage, with GulpR^JF03036

(Barraza et al., 2025)

abnormal immune response | adult stage, with Rel^GD1199

(Jugder et al., 2021)

abnormal immune response | adult stage, with Targ^HMC04734

(Jugder et al., 2021)

abnormal immune response | adult stage, with Tip60^GL00130

(Jugder et al., 2021)

abnormal immune response | adult stage, with Tk^JF01818

(Jugder et al., 2021)

abnormal sleep | heat sensitive, with TrpA1^UAS.cUa

(Chung et al., 2017)

abnormal starvation stress response | adult stage, with GulpR^JF03036

(Barraza et al., 2025)

abnormal starvation stress response | adult stage, with NPF^JF02555

(Yoshinari et al., 2021)

abnormal starvation stress response | adult stage, with NPF^KK111846

(Yoshinari et al., 2021)

abnormal starvation stress response | adult stage, with sut1^HMC06243

(Yoshinari et al., 2021)

abnormal starvation stress response | adult stage, with sut1^KK112749

(Yoshinari et al., 2021)

abnormal starvation stress response | adult stage, with Tk^JF01818

(Barraza et al., 2025)

decreased cell number | adult stage, with AcCoAS^HMS02314

(Jugder et al., 2021)

decreased cell number | adult stage, with Acly^HMC06049

(Jugder et al., 2021)

decreased cell number | adult stage, with Ada3^HMS00450

(Jugder et al., 2021)

decreased cell number | adult stage, with Cs1^HMS01631

(Jugder et al., 2021)

decreased cell number | adult stage, with dom^GLC01875

(Jugder et al., 2021)

decreased cell number | adult stage, with HDAC3^UAS.cNa

(Jugder et al., 2021)

decreased cell number | adult stage, with His2Av^HMS02773

(Jugder et al., 2021)

decreased cell number | adult stage, with NPF^KK111846

(Yoshinari et al., 2021)

decreased cell number | adult stage, with PGRP-LC^HMS00259

(Jugder et al., 2021)

decreased cell number | adult stage, with PGRP-LE^HMC05031

(Jugder et al., 2021)

decreased cell number | adult stage, with pont^HMJ21078

(Jugder et al., 2021)

decreased cell number | adult stage, with Rel^GD1199

(Jugder et al., 2021)

decreased cell number | adult stage, with shd^HMC06461

(Jugder et al., 2021)

decreased cell number | adult stage, with Targ^HMC04734

(Jugder et al., 2021)

decreased cell number | adult stage, with Tip60^GL00130

(Jugder et al., 2021)

decreased cell number | adult stage, with Tk^JF01818

(Barraza et al., 2025)

decreased cell number | oogenesis | conditional, with NPF^JF02555

(Ameku et al., 2018)

decreased fecundity | female | conditional, with NPF^JF02555

(Ameku et al., 2018)

decreased occurrence of cell division | oogenesis | conditional, with NPF^JF02555

(Ameku et al., 2018)

increased cell number | adult stage, with HDAC3^JF01420

(Jugder et al., 2021)

increased cell number | adult stage, with HDAC4^HM05035

(Jugder et al., 2021)

increased cell number | oogenesis | conditional, with Df(3R)ED10642/NPF^sk1, NPF^UAS.cWa

(Ameku et al., 2018)

increased mortality | calorie restriction conditional, with GulpR^JF03036

(Barraza et al., 2025)

increased mortality | calorie restriction conditional, with Tk^JF01818

(Barraza et al., 2025)

increased rate of feeding behavior | adult stage, with NPF^KK111846

(Yoshinari et al., 2021)

long lived | nutrition conditional, with NPF^JF02555

(Chen et al., 2024)

short lived | conditional, with AcCoAS^HMS02314

(Jugder et al., 2021)

short lived | conditional, with Rel^GD1199

(Jugder et al., 2021)

short lived | conditional, with Targ^HMC04734

(Jugder et al., 2021)

short lived | conditional, with Tip60^GL00130

(Jugder et al., 2021)

short lived | conditional, with Tk^JF01818

(Jugder et al., 2021)

Phenotype Manifest In

adult anterior midgut, with AcCoAS^HMS02314

(Jugder et al., 2021)

adult anterior midgut, with Acly^HMC06049

(Jugder et al., 2021)

adult anterior midgut, with Ada3^HMS00450

(Jugder et al., 2021)

adult anterior midgut, with Cs1^HMS01631

(Jugder et al., 2021)

adult anterior midgut, with dom^GLC01875

(Jugder et al., 2021)

adult anterior midgut, with GulpR^HMS05678

(Barraza et al., 2025)

adult anterior midgut, with GulpR^JF03036

(Barraza et al., 2025)

adult anterior midgut, with HDAC3^JF01420

(Jugder et al., 2021)

adult anterior midgut, with HDAC3^UAS.cNa

(Jugder et al., 2021)

adult anterior midgut, with HDAC4^HM05035

(Jugder et al., 2021)

adult anterior midgut, with His2Av^HMS02773

(Jugder et al., 2021)

adult anterior midgut, with PGRP-LC^HMS00259

(Jugder et al., 2021)

adult anterior midgut, with PGRP-LE^HMC05031

(Jugder et al., 2021)

adult anterior midgut, with pont^HMJ21078

(Jugder et al., 2021)

adult anterior midgut, with Rel^GD1199

(Jugder et al., 2021)

adult anterior midgut, with shd^HMC06461

(Jugder et al., 2021)

adult anterior midgut, with Targ^HMC04734

(Jugder et al., 2021)

adult anterior midgut, with Tip60^GL00130

(Jugder et al., 2021)

adult anterior midgut, with Tk^JF01818

(Barraza et al., 2025)

adult anterior midgut | conditional, with PGRP-LC^a.UAS

(Jugder et al., 2021)

adult anterior midgut | conditional, with PGRP-LC^x.UAS

(Jugder et al., 2021)

adult enteroendocrine cell, with GulpR^HMS05678

(Barraza et al., 2025)

adult enteroendocrine cell, with GulpR^JF03036

(Barraza et al., 2025)

adult enteroendocrine cell | decreased number, with AcCoAS^HMS02314

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with Acly^HMC06049

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with Ada3^HMS00450

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with Cs1^HMS01631

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with dom^GLC01875

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with His2Av^HMS02773

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with NPF^KK111846

(Yoshinari et al., 2021)

adult enteroendocrine cell | decreased number, with PGRP-LC^HMS00259

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with PGRP-LE^HMC05031

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with pont^HMJ21078

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with Rel^GD1199

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with shd^HMC06461

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with Targ^HMC04734

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with Tip60^GL00130

(Jugder et al., 2021)

adult enteroendocrine cell | decreased number, with Tk^JF01818

(Barraza et al., 2025)

adult enteroendocrine cell | increased number, with HDAC3^JF01420

(Jugder et al., 2021)

adult enteroendocrine cell | increased number, with HDAC4^HM05035

(Jugder et al., 2021)

adult enteroendocrine cell | increased number | conditional, with PGRP-LC^a.UAS

(Jugder et al., 2021)

adult enteroendocrine cell | increased number | conditional, with PGRP-LC^x.UAS

(Jugder et al., 2021)

adult fat body, with HDAC3^JF01420

(Jugder et al., 2021)

adult fat body, with HDAC4^HM05035

(Jugder et al., 2021)

adult fat body, with Targ^HMC04734

(Jugder et al., 2021)

adult posterior midgut, with GulpR^HMS05678

(Barraza et al., 2025)

adult posterior midgut, with GulpR^JF03036

(Barraza et al., 2025)

adult posterior midgut, with Tk^JF01818

(Barraza et al., 2025)

enterocyte, with Tk^JF01818

(Song et al., 2014)

female germline stem cell | conditional, with Df(3R)ED10642/NPF^sk1, NPF^UAS.cWa

(Ameku et al., 2018)

female germline stem cell | conditional, with NPF^JF02555

(Ameku et al., 2018)

lipid droplet | adult stage, with Ada3^HMS00450

(Jugder et al., 2021)

lipid droplet | adult stage, with dom^GLC01875

(Jugder et al., 2021)

lipid droplet | adult stage, with HDAC3^JF01420

(Jugder et al., 2021)

lipid droplet | adult stage, with HDAC4^HM05035

(Jugder et al., 2021)

lipid droplet | adult stage, with His2Av^HMS02773

(Jugder et al., 2021)

lipid droplet | adult stage, with PGRP-LC^HMS00259

(Jugder et al., 2021)

lipid droplet | adult stage, with PGRP-LE^HMC05031

(Jugder et al., 2021)

lipid droplet | adult stage, with pont^HMJ21078

(Jugder et al., 2021)

lipid droplet | adult stage, with Rel^GD1199

(Jugder et al., 2021)

lipid droplet | adult stage, with shd^HMC06461

(Jugder et al., 2021)

lipid droplet | adult stage, with Targ^HMC04734

(Jugder et al., 2021)

lipid droplet | adult stage, with Tip60^GL00130

(Jugder et al., 2021)

lipid droplet | adult stage | decreased number | conditional, with PGRP-LC^a.UAS

(Jugder et al., 2021)

lipid droplet | adult stage | decreased number | conditional, with PGRP-LC^x.UAS

(Jugder et al., 2021)

lipid droplet | adult stage | increased number, with AcCoAS^HMS02314

(Jugder et al., 2021)

lipid droplet | adult stage | increased number, with HDAC3^UAS.cNa

(Jugder et al., 2021)

Detailed Description

Statement

Reference

Acute thermogenetic activation (using TrpA1^Scer\UAS.cUa at 29[o]C) of Scer\GAL4^Tk.PS cells results in a slight but significant decrease in total sleep amount (particularly during the 12hr dark phases), compared to controls. Acute thermogenetic activation of these neurons does not result in a significant increase of food consumption (tested in a FLIC assay), compared to controls.

(Chung et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

NPF^KK111846, Scer\GAL4^Tk.PS has abnormal starvation stress response | adult stage phenotype, non-enhanceable by Akh^A/Akh[+]

(Yoshinari et al., 2021)

NOT suppressed by