FB2026_02 , released June 18, 2026
Allele: Dmel\NPFsk1
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General Information
Symbol
Dmel\NPFsk1
Species
D. melanogaster
Name
FlyBase ID
FBal0343402
Feature type
allele
Associated gene
Dmel\NPF
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Ameku et al., 2018)
Progenitor genotype
Cytology
Description

Mutation within the NPF coding sequence; deletion of 179 nucleotides plus insertion of 2 nucleotides.

(Ameku et al., 2018)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
3R:16,610,834..16,611,011 [+]
Comment:

A 178bp deletion and two base insertion (GG) in the NPF coding sequence leads to a frameshift and early translation termination.

Inserted_sequence:

GG

(Ameku et al., 2018)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      NPFsk1/Df(3R)ED10642 transheterozygous females fail to show the expected mating-induced increase in germline stem cells in the germarium, as compared to controls; the expression of NPFUAS.cWa under the control of Scer\GAL4Tk.PS rescues the germline stem cell number in mated females, but also leads to an abnormal increase in germline stem cells in virgin females.

      (Ameku et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      NPFSK1
      (Araki et al., 2020)
      NPFsk1
      (Chen et al., 2023, Yoshinari et al., 2021, Ameku et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)