FlyBase Allele Report: Dmel\Tk[UAS.cAa]

General Information

Symbol

Dmel\Tk^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Asahina

FlyBase ID

FBal0295792

Feature type

allele

Associated gene

Dmel\Tk

Associated Insertion(s)

Carried in Construct

P{UAS-Tk.A}

Also Known As

UAS-Tk

Key Links

Transgenic product class

wild_type

(Asahina et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Asahina et al., 2014)

Mutagen

in vitro construct

(Asahina et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-Tk.A}

Cytology

Description

UAS regulatory sequences drive expression of Tk.

(Asahina et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Tk^UAS.cAa/Scer\GAL4^Tk is an enhancer of abnormal behavior | adult stage | heat sensitive phenotype of Scer\GAL4^Tk, TrpA1^UAS.(B).cKa

(Asahina et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Tk^Scer\UAS.cAa enhances the aggression-promoting effect of activating neurons labeled with the P{Tk-GAL4.TH}5Fa insertion of Scer\GAL4^Tk through expression of thermosensitive TrpA1^{Scer\UAS.(B).cKa}. The "dose-response" curve for behavior versus temperature is shifted to the left.

(Asahina et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Tk^UAS.cAa/Scer\GAL4^Tk rescues Df(3R)ΔTk²/Tk^Δ1

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^dimm-929 rescues Df(3R)ΔTk²/Tk^Δ1

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^Tk rescues Df(3R)ΔTk²

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^dimm-929 rescues Df(3R)ΔTk²

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^Tk rescues Df(3R)ΔTk²/Tk^Δ1

(Asahina et al., 2014)

Tk^UAS.cAa/Scer\GAL4^GMR61H07 rescues Df(3R)ΔTk²/Tk^Δ1

(Asahina et al., 2014)

Fails to rescue

Tk^UAS.cAa/Scer\GAL4^NPF.1 fails to rescue Tk^Δ1

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^dimm-929 fails to rescue Tk^Δ1

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^NPF.1 fails to rescue Df(3R)ΔTk²

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^Tk fails to rescue Df(3R)ΔTk²/Tk^Δ1

(Asahina et al., 2014)

Comments

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^Tk rescues the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in Tk^Δ1/Df(3R)ΔTk² mutant flies. As in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^Tk rescues the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in homozygous Df(3R)ΔTk² mutant flies. As in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503. Expressing Tk^Scer\UAS.cAa using Scer\GAL4^NPF.1 does not restore CH503 sensitivity.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^NPF.1 does not rescue the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in homozygous Df(3R)ΔTk² mutant flies. As in Df(3R)ΔTk² mutants alone, the courtship defects observed in response to CH503 in wild type are not seen.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^NPF.1 does not rescue the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in homozygous Tk^Δ1 mutant flies. As in Tk^Δ1 mutants alone, the courtship defects observed in response to CH503 in wild type are not seen.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^dimm-929 rescues the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in homozygous Df(3R)ΔTk² mutant flies. As in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^dimm-929 rescues the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in Tk^Δ1/Df(3R)ΔTk² mutant flies. As in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^dimm-929 does not rescue the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in homozygous Tk^Δ1 mutant flies. As in Tk^Δ1 mutants alone, the courtship defects observed in response to CH503 in wild type are not seen.

(Shankar et al., 2015)

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^Tk (either the P{Tk-GAL4.TH}5Fa or P{Tk-GAL4.TH}3Ma insertion) fully rescues the defects in aggressive behavior seen in Tk^Δ1/Df(3R)ΔTk² mutant males. No rescue is seen when Tk^Scer\UAS.cAa is driven by another insertion of the same construct, P{Tk-GAL4.TH}2Ma, or Scer\GAL4^GMR61H07.

(Asahina et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600322

w¹¹¹⁸; P{UAS-Tk.A}attP40/CyO; TM2/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tk^Scer\UAS.cAa

Tk^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Asahina

Secondary FlyBase IDs

References (3)

Report Sections

Open Close