FlyBase Allele Report: Scer\GAL4[Tk]

General Information

Symbol

Scer\GAL4^Tk

Species

S. cerevisiae

Name

FlyBase ID

FBal0288518

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Tk-GAL4.TH}

Also Known As

Tk-GAL4, TK-GAL4¹

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Asahina and Anderson, 2013.9.23)

Progenitor genotype

Carried in construct

P{Tk-GAL4.TH}

Cytology

Description

Putative Tk regulatory sequences control expression of a GAL4 driver.

(Asahina and Anderson, 2013.9.23)

Allele components

Component

Use(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

adult fruitless aSP-f (male) neuron | subset

(Asahina et al., 2014)

adult fruitless aSP-g (male) neuron | subset

(Asahina et al., 2014)

adult ventral nerve cord | restricted

(Asahina et al., 2014)

adult stage | female

adult fruitless aSP-f (female) neuron | subset

(Asahina et al., 2014)

adult fruitless aSP-g (female) neuron | subset

(Asahina et al., 2014)

adult stage

adult protocerebral LPP1 Tachykinin neuron | subset

Comment: 6 neurons

(Asahina et al., 2014)

tachykinin neuron

(Shankar et al., 2015)

Additional Information

Statement

Reference

ScerGAL4^Tk drives expression in 7 NPF positive tachykinin neurons in the anterior ventrolateral protocerebrum.

(Shankar et al., 2015)

ScerGAL4^Tk drives expression in around six male adult protocerebral LPP2 Tachykinin neurons that display a ring-shaped arborization pattern. Three to four of these neurons co-express the male-specific isoform of EcollexA^{fru.P1.T:HsimVP16}. A subset of ScerGAL4^Tk-positive neurons co-express Tk-protein. Male-specific labeling is also observed in the cells of the thoracico-abdominal ganglion.

(Asahina et al., 2014)

Marker for

Reflects expression of

(Shankar et al., 2015, Asahina et al., 2014)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Ablation of Scer\GAL4^Tk-expressing neurons using either the P{Tk-GAL4.TH}3Ma or P{Tk-GAL4.TH}2Ma insertion results in reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). In contrast to wild type, flies do not exhibit courtship defects in the presence of (S,Z,Z)-CH503. Ablation of cells expressing using the P{Tk-GAL4.TH}5Fa insertion does not lead to reduced sensitivity to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Thermogenetic activation of neurons labelled by Scer\GAL4^Tk (either the P{Tk-GAL4.TH}5Fa or P{Tk-GAL4.TH}3Ma insertion) through expression of TrpA1^{Scer\UAS.(B).cKa} at 6 days after eclosion strongly increases male aggression compared to controls. No increase in aggression is seen when TrpA1^{Scer\UAS.(B).cKa} is expressed under the control of another insertion of the same construct, P{Tk-GAL4.TH}2Ma. Activation of P{Tk-GAL4.TH}5Fa neurons also increase aggression towards a wild-type male.

Thermogenetic activation of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4^Tk and Ecol\lexA^{fru.P1.T:Hsim\VP16}) increases male-male aggression compared to controls. Ecol\lexA^{fru.P1.T:Hsim\VP16} drives expression of Scer\FLP1^{L.8xEcol\lexAop}, which results in excision of the Scer\FRT cassette from TrpA1^{Scer\FRT.stop.Scer\UAS.T:Disc\RFP-mCherry} enabling these cells to express TrpA1 under the control of Scer\GAL4^Tk when the flies are shifted to the permissive temperature. Activation of Tk-GAL4[Fru[M] neurons does not induce aggression of male flies towards females, and they copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged.

Thermogenetic activation of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4^Tk and Ecol\lexA^{fru.P1.T:Hsim\VP16}) increases male-male aggression compared to controls. Ecol\lexA^{fru.P1.T:Hsim\VP16} drives expression of Scer\FLP1^{L.8xEcol\lexAop}, which results in excision of the Scer\FRT cassette from TrpA1^{Scer\FRT.stop.Scer\UAS.T:Hsap\MYC} enabling these cells to express TrpA1 under the control of Scer\GAL4^Tk when the flies are shifted to the permissive temperature. There is no change in male-male courtship behavior; the frequency of unilateral wing extension or "singing" towards other males is unchanged.

Silencing of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4^Tk and Ecol\lexA^{fru.P1.T:Hsim\VP16}) reduces levels of male-male aggression compared to controls, although the level of baseline aggression between different controls varies. Ecol\lexA^{fru.P1.T:Hsim\VP16} drives expression of Scer\FLP1^{L.8xEcol\lexAop}, which results in excision of the Scer\FRT cassette from Hsap\KCNJ2^{Scer\FRT.Stop.Scer\UAS.T:Avic\GFP-EGFP} enabling these cells to express Hsap\KCNJ2 under the control of Scer\GAL4^Tk. The males copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged. Silencing of Tk-GAL4[Fru[M] neurons does not consistently influence the frequency of unilateral wing extensions towards other males in comparison with control strains.

(Asahina et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Tk, TrpA1^UAS.(B).cKa has abnormal behavior | adult stage | heat sensitive phenotype, enhanceable by Tk^UAS.cAa/Scer\GAL4^Tk

(Asahina et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^Tk, TrpA1^UAS.(B).cKa has abnormal behavior | adult stage | heat sensitive phenotype, suppressible | partially by Df(3R)ΔTk²/Tk^Δ1

(Asahina et al., 2014)

Scer\GAL4^Tk, TrpA1^UAS.(B).cKa has abnormal behavior | adult stage | heat sensitive phenotype, suppressible by TkR86C^ΔF28

(Asahina et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^Tk, TrpA1^UAS.(B).cKa has abnormal behavior | adult stage | heat sensitive phenotype, non-suppressible by TkR99D^MB09356

(Asahina et al., 2014)

Enhancer of

Statement

Reference

Hsap\KCNJ2^UAS.EGFP/Scer\GAL4^Tk is an enhancer of abnormal courtship behavior | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Ecol\lexA^GMR15A01

(Hoopfer et al., 2015)

Tk^UAS.cAa/Scer\GAL4^Tk is an enhancer of abnormal behavior | adult stage | heat sensitive phenotype of Scer\GAL4^Tk, TrpA1^UAS.(B).cKa

(Asahina et al., 2014)

Suppressor of

Statement

Reference

Hsap\KCNJ2^UAS.EGFP/Scer\GAL4^Tk is a suppressor | partially of abnormal behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Ecol\lexA^GMR15A01

(Hoopfer et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Tk^Scer\UAS.cAa enhances the aggression-promoting effect of activating neurons labeled with the P{Tk-GAL4.TH}5Fa insertion of Scer\GAL4^Tk through expression of thermosensitive TrpA1^{Scer\UAS.(B).cKa}. The "dose-response" curve for behavior versus temperature is shifted to the left.

Tk^Δ1/Df(3R)ΔTk² partially suppresses the aggression-promoting effect of activating neurons labeled with the P{Tk-GAL4.TH}5Fa insertion of Scer\GAL4^Tk through expression of thermosensitive TrpA1^{Scer\UAS.(B).cKa}.

Homozygous TkR86C^ΔF28 significantly suppresses the aggression-promoting effect of activating neurons labeled with Scer\GAL4^Tk (either the P{Tk-GAL4.TH}5Fa or P{Tk-GAL4.TH}3Ma insertion) through expression of thermosensitive TrpA1^{Scer\UAS.(B).cKa}. This suppression is partially reversed upon expression of TkR86C^{+tCH322-17N40}.

Homozygous TkR99D^MB09356 does not suppress the aggression-promoting effect of activating neurons labeled with Scer\GAL4^Tk (either the P{Tk-GAL4.TH}5Fa or P{Tk-GAL4.TH}3Ma insertion) through expression of thermosensitive TrpA1^{Scer\UAS.(B).cKa}.

(Asahina et al., 2014)

Xenogenetic Interactions

Statement

Reference

Inhibition of Scer\GAL4^Tk cells using Hsap\KCNJ2^UAS.EGFP significantly increases wing extensions towards other males after, but not during, light activation of Ecol\lexA^GMR15A01 cells using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, compared to activation alone.

(Hoopfer et al., 2015)

Complementation and Rescue Data

Rescues

Tk^UAS.cAa/Scer\GAL4^Tk rescues Df(3R)ΔTk²/Tk^Δ1

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^Tk rescues Df(3R)ΔTk²

(Shankar et al., 2015)

Tk^UAS.cAa/Scer\GAL4^Tk rescues Df(3R)ΔTk²/Tk^Δ1

(Asahina et al., 2014)

Fails to rescue

Tk^UAS.cAa/Scer\GAL4^Tk fails to rescue Df(3R)ΔTk²/Tk^Δ1

(Asahina et al., 2014)

Comments

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^Tk (either the P{Tk-GAL4.TH}5Fa or P{Tk-GAL4.TH}3Ma insertion) fully rescues the defects in aggressive behavior seen in Tk^Δ1/Df(3R)ΔTk² mutant males. No rescue is seen when Tk^Scer\UAS.cAa is driven by another insertion of the same construct, P{Tk-GAL4.TH}2Ma, or Scer\GAL4^GMR61H07.