Thermogenetic activation of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4Tk and Ecol\lexAfru.P1.T:Hsim\VP16) increases male-male aggression compared to controls. Ecol\lexAfru.P1.T:Hsim\VP16 drives expression of Scer\FLP1L.8xEcol\lexAop, which results in excision of the Scer\FRT cassette from TrpA1Scer\FRT.stop.Scer\UAS.T:Disc\RFP-mCherry enabling these cells to express TrpA1 under the control of Scer\GAL4Tk when the flies are shifted to the permissive temperature. Activation of Tk-GAL4[Fru[M] neurons does not induce aggression of male flies towards females, and they copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged.