FlyBase Allele Report: Dmel\Tk[Δ1]

General Information

Symbol

Dmel\Tk^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0295791

Feature type

allele

Associated gene

Dmel\Tk

Associated Insertion(s)

P+PBac{XP3.WH3}Tk^Δ1

Carried in Construct

Also Known As

ΔTk¹

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Asahina et al., 2014)

Mutagen

FLPase

(Asahina et al., 2014)

Progenitor genotype

PBac{WH}Tk^f03824

(Asahina et al., 2014)

P{XP}d08303

(Asahina et al., 2014)

Associated Insertion(s)

P+PBac{XP3.WH3}Tk^Δ1

Cytology

Description

Scer\FLP1 mediated chromosome translocation using PBac{WH}Tk^f03824 and P{XP}d08303 has resulted in deletion of Tk. The expression of a neighboring gene, mfas, is not affected.

(Asahina et al., 2014)

Allele components

Component

Use(s)

Inserted element

P+PBac{XP3.WH3}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:11,993,399..11,997,845 [-]

Comment:

Deletion resulting from Scer\FLP1 mediated chromosome translocation using PBac{WH}Tk^f03824 and P{XP}d08303, which removes the coding region of Tk.

(Asahina et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage (with Df(3R)ΔTk²)

(Asahina et al., 2014)

abnormal behavior | adult stage | recessive

(Asahina et al., 2014)

abnormal courtship behavior | chemical conditional

(Shankar et al., 2015)

abnormal courtship behavior | chemical conditional (with Df(3R)ΔTk²)

(Shankar et al., 2015)

fertile

(Asahina et al., 2014)

viable

(Asahina et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous Tk^Δ1 mutant flies exhibit reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503): in contrast to wild type they do not exhibit increased courtship defects in the presence of (S,Z,Z)-CH503 compared with controls.

Tk^Δ1/Df(3R)ΔTk² mutant flies exhibit reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503): in contrast to wild type they do not exhibit increased courtship defects in the presence of (S,Z,Z)-CH503 compared with controls.

(Shankar et al., 2015)

Tk^Δ1 mutant flies are fully viable and fertile and exhibit no obvious anatomical abnormalities of differences from controls in locomotor activity. Homozygotes show a significant decrease in male-male aggressive behavior compared to heterozygous controls. Males copulate with virgin females at efficiencies comparable to those observed for heterozygous control males. Copulation latency and latency are unaffected, as is the frequency of unilateral wing extension "singing" toward mated females. The frequency of unilateral wing extension toward other males is also not consistently different from controls.

Tk^Δ1/Df(3R)ΔTk² transheterozygotes show a significant decrease in male-male aggressive behavior compared to heterozygous controls. Males copulate with virgin females at efficiencies comparable to those observed for heterozygous control males. Copulation latency and latency are unaffected, as is the frequency of unilateral wing extension "singing" toward mated females. The frequency of unilateral wing extension toward other males is also not consistently different from controls.

(Asahina et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Df(3R)ΔTk²/Tk^Δ1 is a suppressor | partially of abnormal behavior | adult stage | heat sensitive phenotype of Scer\GAL4^Tk, TrpA1^UAS.(B).cKa

(Asahina et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Tk^Δ1/Df(3R)ΔTk² partially suppresses the aggression-promoting effect of activating neurons labeled with the P{Tk-GAL4.TH}5Fa insertion of Scer\GAL4^Tk through expression of thermosensitive TrpA1^{Scer\UAS.(B).cKa}.

(Asahina et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(3R)ΔTk²/Tk^Δ1 is rescued by Tk^UAS.cAa/Scer\GAL4^Tk

(Shankar et al., 2015)

Df(3R)ΔTk²/Tk^Δ1 is rescued by Tk^UAS.cAa/Scer\GAL4^dimm-929

(Shankar et al., 2015)

Df(3R)ΔTk²/Tk^Δ1 is rescued by Tk^UAS.cAa/Scer\GAL4^Tk

(Asahina et al., 2014)

Df(3R)ΔTk²/Tk^Δ1 is rescued by Tk^UAS.cAa/Scer\GAL4^GMR61H07

(Asahina et al., 2014)

Not rescued by

Tk^Δ1 is not rescued by Tk^UAS.cAa/Scer\GAL4^NPF.1

(Shankar et al., 2015)

Tk^Δ1 is not rescued by Tk^UAS.cAa/Scer\GAL4^dimm-929

(Shankar et al., 2015)

Df(3R)ΔTk²/Tk^Δ1 is not rescued by Tk^UAS.cAa/Scer\GAL4^Tk

(Asahina et al., 2014)

Comments

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^Tk rescues the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in Tk^Δ1/Df(3R)ΔTk² mutant flies. As in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^NPF.1 does not rescue the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in homozygous Tk^Δ1 mutant flies. As in Tk^Δ1 mutants alone, the courtship defects observed in response to CH503 in wild type are not seen.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^dimm-929 does not rescue the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in homozygous Tk^Δ1 mutant flies. As in Tk^Δ1 mutants alone, the courtship defects observed in response to CH503 in wild type are not seen.

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^dimm-929 rescues the reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503) seen in Tk^Δ1/Df(3R)ΔTk² mutant flies. As in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Expression of Tk^Scer\UAS.cAa under the control of Scer\GAL4^Tk (either the P{Tk-GAL4.TH}5Fa or P{Tk-GAL4.TH}3Ma insertion) fully rescues the defects in aggressive behavior seen in Tk^Δ1/Df(3R)ΔTk² mutant males. No rescue is seen when Tk^Scer\UAS.cAa is driven by another insertion of the same construct, P{Tk-GAL4.TH}2Ma, or Scer\GAL4^GMR61H07.

(Asahina et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600321

w¹¹¹⁸; Bl¹/CyO; P+PBac{XP3.WH3}Tk^Δ1/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Tk^Δ1

Δ1TK1

(Shankar et al., 2015)

Δ^Tk1

(Wohl et al., 2023)

ΔTk¹

(Tsuji et al., 2023, Asahina et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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