Expression of Tk^KK112227 driven by Scer\GAL4^elav-C155 does not significantly alter baseline thermal nociception (uninjured early third instar larval responses to thermal probes at 45[o] or 48[o]C) compared to controls. 24 hours after UV-induced nociceptive sensitization, larvae with expression of Tk^KK112227 driven by Scer\GAL4^elav-C155 (but not Scer\GAL4^ppk.1.9) show significant reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe) compared to controls.

Expression of Tk^KK112227 under the control of Scer\GAL4^nSyb.PU (in combination with UAS-Dicer2 to increase RNAi efficiency) results in a significant increase in the latency to sperm ejection in females after the end of copulation.

Flies expressing Tk^KK112227 under the control of Scer\GAL4^NPF.1 (in which expression is suppressed in the ventral nerve cord using Scer\GAL80^tsh-GAL80) exhibit reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503): in contrast to wild type they do not exhibit increased courtship defects in the presence of (S,Z,Z)-CH503 compared with controls.

When Tk^KK112227 is expressed under the control of Scer\GAL4^NPF.1 from the late pupal stage onwards (using Scer\GAL80^ts.αTub84B) reduced sensitivity is seen compared to controls.

Flies expressing Tk^KK112227 under the control of Scer\GAL4^dimm-929 exhibit reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503): in contrast to wild type they do not exhibit increased courtship defects in the presence of (S,Z,Z)-CH503 compared with controls.