ScerGAL4^{5-HT2-PL00052} is expressed in commissures connecting the left and right hemispheres of the early third instar larval brain, in clusters of cells within the medial regions of the hemispheres, a small population of cells within the developing optic ganglia, and in a small number of cells in the ventral ganglia. In the pupal brain, the larval expression pattern of ScerGAL4^{5-HT2-PL00052} is replaced by expression in a disc of cells at the base of the developing optic ganglia and in cells that may be glia. In the adults, expression of ScerGAL4^{5-HT2-PL00052} is seen in cells in the protocerebrum that include multipolar neurons around the calyces. It is also expressed in gustatory neuron that project into the subesophageal ganglion.