Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection; some lines were mapped by the GDP.
A set of transgenic insertion stocks derived by TE mobilization using the "piggyBac mutator" construct PBac{GAL4D,EYFP}. The dataset consists primarily of insertions on the third chromosomes that also carry proximal Scer\FRT sites, to allow generation of germ-line clones. The PBac{GAL4D,EYFP} construct carries the yellow fluorescent marker Avic\GFPEYFP.3xP3 (which can be distinguished from other GFP-based markers) and a modified Scer\GAL4 driver/reporter gene.
ScerGAL45-HT2-PL00052 is expressed in commissures connecting the left and right hemispheres of the early third instar larval brain, in clusters of cells within the medial regions of the hemispheres, a small population of cells within the developing optic ganglia, and in a small number of cells in the ventral ganglia. In the pupal brain, the larval expression pattern of ScerGAL45-HT2-PL00052 is replaced by expression in a disc of cells at the base of the developing optic ganglia and in cells that may be glia. In the adults, expression of ScerGAL45-HT2-PL00052 is seen in cells in the protocerebrum that include multipolar neurons around the calyces. It is also expressed in gustatory neuron that project into the subesophageal ganglion.
PL00052-5prime
