FlyBase Allele Report: Dmel\5-HT2A[PL00052]

General Information

Symbol

Dmel\5-HT2A^PL00052

Species

D. melanogaster

Name

FlyBase ID

FBal0162863

Feature type

allele

Associated gene

Dmel\5-HT2A

Associated Insertion(s)

PBac{GAL4D,EYFP}5-HT2A^PL00052

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

PBac{GAL4D,EYFP}5-HT2A^PL00052

Cytology

Description

PBac{GAL4D,EYFP} insertion within the second intron.

(Nichols, 2007)

Allele components

Component

Use(s)

Inserted element

PBac{GAL4D,EYFP}

enhancer trap

Encoded product / tool

GAL4(II-9)

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:4,624,429..4,624,429 [+]

(Huser et al., 2017, Johnson et al., 2011, Nichols, 2007, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous 5-HT2A^PL00052 larvae have a significant reduction in aversive (salt), but not appetitive (fructose) olfactory memory, compared to controls, with no effect on naive preference for olfactory or gustatory stimuli.

(Huser et al., 2017)

Unlike wild type, 5-HT2A^PL00052 larvae are resistant to 100μM metitepine, and show relatively higher feeding rates when fed with this drug.

Unlike wild type, 5-HT2A^PL00052/5-HT2A^Gal4 larvae are resistant to 100μM metitepine, and show relatively higher feeding rates when fed with this drug.

(Gasque et al., 2013)

5-HT2^PL00052 mutants exhibit an approximate 50% reduction in short-term memory.

5-HT2^PL00052 mutants do not exhibit a deficit in odor or shock reactivity.

(Johnson et al., 2011)

Flies homozygous for 5-HT2^PL00052 do not show increases in activity in the first half of the day in response to 3mM (R)-DOI ((R)-1-^{2,5-dimethoxy-4-iodophenyl}-2-aminopropane), unlike control flies. Homozygotes show a reduction in anticipatory behaviour in response to 3mM (R)-DOI, but the behaviour is not eliminated.

(Nichols, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

19367

w^*; P{FRT(w^hs)}2A P{neoFRT}82B PBac{GAL4D,EYFP}5-HT2A^PL00052

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

5-HT2^PL00052

(Nichols, 2007)

5-HT2A^PL00052

(Park et al., 2018, Gasque et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close