FlyBase Allele Report: Scer\GAL4[Trhn.PB]

General Information

Symbol

Scer\GAL4^Trhn.PB

Species

S. cerevisiae

Name

Tryptophan hydroxylase promoter construct of Birman

FlyBase ID

FBal0280055

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Trhn-GAL4.B}

Also Known As

TRH-GAL4, TRHGAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Sadaf et al., 2012)

Progenitor genotype

Carried in construct

P{Trhn-GAL4.B}

Cytology

Description

1.9 kb and a 3 kb genomic fragments, containing sequences located upstream and downstream the Trh transcription start site, regulate expression of a GAL4 driver.

(Cassar et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

Trhn

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

serotonergic neuron

(Sadaf et al., 2012)

adult stage

serotonergic neuron

(Sadaf et al., 2012)

serotonergic neuron | subset of adult brain

(Riemensperger et al., 2013)

Additional Information

Statement

Reference

Expression is driven in around 75% of the adult brain serotonergic system.

(Riemensperger et al., 2013)

Marker for

Reflects expression of

Trhn

(Riemensperger et al., 2013, Sadaf et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Ctet\tetX^TNT-LC.UAS

(Sadaf et al., 2012)

abnormal flight, with Hsap\KCNJ2^UAS.EGFP

(Sadaf et al., 2012)

abnormal flight | heat sensitive, with shi^1.UAS

(Sadaf et al., 2012)

abnormal memory | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Galili et al., 2014)

abnormal memory | adult stage | cold sensitive, with Rnor\Trpm8^UAS.EGFP

(Sitaraman et al., 2017)

abnormal memory | adult stage | conditional, with Ctet\tetX^TNT-LC.UAS

(Sitaraman et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL80^SerT.Si6, TrpA1^UAS.(B).cKa

(Sitaraman et al., 2017)

abnormal memory | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Sitaraman et al., 2017)

abnormal neuroanatomy, with PolG1^KK100485

(Humphrey et al., 2012)

abnormal neuroanatomy | adult stage, with Ctet\tetX^TNT-LC.UAS

(Sadaf et al., 2012)

abnormal neurophysiology, with Ctet\tetX^TNT-LC.UAS

(Sadaf et al., 2012)

abnormal neurophysiology, with Hsap\KCNJ2^UAS.EGFP

(Sadaf et al., 2012)

abnormal neurophysiology | heat sensitive, with shi^1.UAS

(Sadaf et al., 2012)

abnormal thermotaxis | adult stage | cold sensitive, with Rnor\Trpm8^UAS.EGFP

(Sitaraman et al., 2017)

abnormal thermotaxis | adult stage | conditional, with Ctet\tetX^TNT-LC.UAS

(Sitaraman et al., 2017)

abnormal thermotaxis | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Sitaraman et al., 2017)

chemical resistant, with ple^UAS.cFa

(Cassar et al., 2015)

viable, with Dop1R2^UAS.cHa

(Cassar et al., 2015)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell, with Ctet\tetX^IMPTNT-V.UAS

(Sadaf et al., 2012)

dorsal longitudinal indirect flight muscle cell, with Itpr^NIG.1063R

(Sadaf et al., 2012)

dorsal longitudinal indirect flight muscle cell, with Orai^GD3554

(Sadaf et al., 2012)

serotonergic neuron, with PolG1^KK100485

(Humphrey et al., 2012)

serotonergic thoracic neuron, with Ctet\tetX^TNT-LC.UAS

(Sadaf et al., 2012)

Detailed Description

Statement

Reference

Ablation (using hid^UAS.cUa and rpr^UAS.cUa) of Scer\GAL4^Trh.PB cells significantly reduces aversive (salt), olfactory memory in larvae, compared to controls, with no effect on naive preference for olfactory or gustatory stimuli.

Acute inhibition (using shi^1.UAS at 35[o]C) of Scer\GAL4^Trh.PB cells in larvae during training and testing does not affect aversive (salt) olfactory memory or naive preference for olfactory or gustatory stimuli, compared to controls.

(Huser et al., 2017)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 31-33[o]C) of Scer\GAL4^Trh.PB cells in adults in a particular position within a chamber results in a significant increase in associative aversive (heat) place memory, compared to controls; increased memory is also seen, to a significantly reduced extent, when activation (at 32[o]C) is restricted to a smaller subset of Scer\GAL4^Trh.PB cells using Scer\GAL80^SerT.Si6.

Preactivation (using TrpA1^UAS.(B).cKa at 32[o]C, or Rnor\Trpm8^UAS.EGFP at 15[o]C, before training) of Scer\GAL4^Trh.PB cells significantly increases latency of escape from heat and associative aversive (heat) place memory, compared to controls.

Inhibition (using Ctet\tetX^TNT.UAS) of Scer\GAL4^Trh.PB cells does not affect latency of escape from heat or associative aversive (heat) place memory, compared to controls; the increases in escape latency and aversive memory due to unexpected high (41[o]C) temperature pre-exposure are significantly lower than those of controls.

(Sitaraman et al., 2017)

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^Trh.PB cells in adults does not affect 5 min (single-trial conditioned) aversive taste memory, compared to controls.

(Kirkhart and Scott, 2015)

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^Trh.PB cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, significantly increases aversive shock, but not temperature, olfactory memory, compared to controls, with no effect on temperature avoidance.

(Galili et al., 2014)

Ablation (using hid^UAS.cUa and rpr^UAS.cUa) of Scer\GAL4^Trh.PB cells in larvae does not significantly affect naive preferences for odors, darkness, salt, or low sugar concentrations, but significantly reduces preference for higher concentrations (2M) of fructose, compared to controls; there is no effect on appetitive or aversive olfactory memory, compared to controls.

(Huser et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^Trh.PB

Scer\GAL4^Trhn.PB

Name Synonyms

Tryptophan hydroxylase promoter construct of Birman

Secondary FlyBase IDs

References (19)

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