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General Information
Symbol
Dmel\PolG1KK100485
Species
D. melanogaster
Name
FlyBase ID
FBal0230781
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of tamKK100485 under the control of Scer\GAL4bam.T:Hsim\VP16 prevents the elimination of mitochondrial nucleoids (containing the mtDNA) in developing spermatids.

Expression of tamKK100485 under the control of Scer\GAL4Act5C.PU results in reduced complex I activity and ATP levels compared with controls. Flies have a significantly shortened lifespan compared to controls.

Flies expressing tamKK100485 under the control of Scer\GAL4tub.PU (limited to the adult stages using Scer\GAL80ts.αTub84B) have a significantly shortened lifespan compared to controls.

Flies expressing tamKK100485 in cholinergic neurons under the control of Scer\GAL4Cha.7.4 (co-expressed with Dcr-2Scer\UAS.cDa) show an age-related decline in climbing performance similar to controls. Flies do not exhibit any differences in spontaneous activity, speed of walking or total distance traveled. No neuron loss is seen in the brain of these flies.

Flies expressing tamKK100485 in dopaminergic neurons under the control of Scer\GAL4ple.PF (co-expressed with Dcr-2Scer\UAS.cDa) show a significant and progressive age-related decline in climbing performance when compared with controls. These flies have a normal lifespan but show decreased activity and, when moving, they walk at a slower speed and cover less distance compared with controls. Expression under the control of Scer\GAL4ple.PF results in a significant and age-related decrease in dopaminergic neurons. PPL1 and PPM1/2 dopaminergic neuron clusters show progressive loss of neurons from 31 to 70 days, whereas PPM3 and PAL clusters show significant early loss( at 31 days) but no significant difference is seen with age.

When compared with age-matched controls, flies expressing tamKK100485 in serotonergic neurons under the control of Scer\GAL4Trh.PB (co-expressed with Dcr-2Scer\UAS.cDa) do not show decreased climbing performance. A small but significantly significant reduction in activity is seen in aged flies. Serotonergic neuron loss is seen only in aged flies. No neuron loss is seen at 5 days, but significant loss is present by 31 days.

A significant loss of dopaminergic neurons, but not serotonergic neurons, is seen in 60 day old flies expressing tamKK100485 under the control of Scer\GAL4elav.PU (co-expressed with Dcr-2Scer\UAS.cDa). Cell loss affects all dopaminergic clusters except PAL.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Phenotype Manifest In
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

Expression of parkScer\UAS.cHa partially suppresses the lethality seen when tamKK100485 is expressed under the control of Scer\GAL4Act5C.PU.

Expression of Drp1Scer\UAS.cDa partially suppresses the lethality seen when tamKK100485 is expressed under the control of Scer\GAL4Act5C.PU.

Expression of dhdScer\UAS.cUa partially suppresses the lethality seen when tamKK100485 is expressed under the control of Scer\GAL4Act5C.PU.

Expression of pleScer\UAS.cFa does not suppress the lethality seen when tamKK100485 is expressed under the control of Scer\GAL4Act5C.PU.

Xenogenetic Interactions
Statement
Reference

Expression of Scer\NDI1Scer\UAS.cSa partially suppresses the lethality seen when tamKK100485 is expressed under the control of Scer\GAL4Act5C.PU.

Expression of Zzzz\AOXScer\UAS.cFa completely suppresses the lethality seen when tamKK100485 is expressed under the control of Scer\GAL4Act5C.PU. The reduction in total ATP content is also suppressed.

Expression of Zzzz\AOXScer\UAS.cFa completely suppresses the dopaminergic neuron loss seen when tamKK100485 is expressed under the control of Scer\GAL4ple.PF.

Complementation and Rescue Data
Partially rescued by
Comments

The failure to eliminate mitochondrial nucleoid and mtDNA during spermatid development characteristic for males expressing tamKK100485 under the control of Scer\GAL4bam.T:Hsim\VP16 can be fully rescued by co-expression of either tamUASp.RR.Tag:FLAG or tamD263A.UASp.RR.Tag:FLAG and is partially rescued by co-expression of tamE813V.UASp.RR.Tag:FLAG but the spermatids still retain some nucleoids during late individualization stage.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PolG1KK100485
tamKK100485
Name Synonyms
Secondary FlyBase IDs
    References (4)