Open Close
Reference
Citation
Yu, Z., O'Farrell, P.H., Yakubovich, N., DeLuca, S.Z. (2017). The Mitochondrial DNA Polymerase Promotes Elimination of Paternal Mitochondrial Genomes.  Curr. Biol. 27(7): 1033--1039.
FlyBase ID
FBrf0235196
Publication Type
Research paper
Abstract

Mitochondrial DNA (mtDNA) is typically inherited from only one parent 1-3. In animals, this is usually the mother. Maternal inheritance is often presented as the passive outcome of the difference in cytoplasmic content of egg and sperm; however, active programs enforce uniparental inheritance at two levels, eliminating paternal mitochondrial genomes or destroying mitochondria delivered to the zygote by the sperm 4-13. Both levels operate in Drosophila <up>8, 12, 13</up>. As sperm formation begins, hundreds of doomed mitochondrial genomes are visualized within the two huge mitochondria of each spermatid. These genomes abruptly disappear during spermatogenesis. Genome elimination, which is not in the interests of the restricted genomes, is directed by nuclear genes. Mutation of EndoG, which encodes a mitochondria-targeted endonuclease, retarded elimination 8. Here, we show that knockdown of the nuclear-encoded mtDNA polymerase (Pol γ-α), Tamas, produces a more complete block of mtDNA elimination. Tamas is found in large particles that localize to mtDNA during genome elimination. We discount a simple possible mechanism by showing that the 3'-exonuclease function of the polymerase is not needed. While DNA elimination is a surprising function for DNA polymerase, it could provide a robust nexus for nuclear control of mitochondrial genome copy number, since use of common interactions for elimination and replication might limit options for the mitochondrial genome to escape restriction. We suggest that the DNA polymerase may play this role more widely and that inappropriate activation of its elimination ability might underlie association of DNA loss syndromes with mutations of the human mtDNA polymerase 14-16.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC5391691 (PMC) (EuropePMC)
Related Publication(s)
Note

Inheritance: Male mtDNA Just Can't Catch a Break.
Patel, 2017, Curr. Biol. 27(7): R264--RR266 [FBrf0235261]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Curr. Biol.
    Title
    Current Biology
    Publication Year
    1991-
    ISBN/ISSN
    0960-9822
    Data From Reference