FlyBase Allele Report: Dmel\Itpr[NIG.1063R]

General Information

Symbol

Dmel\Itpr^NIG.1063R

Species

D. melanogaster

Name

FlyBase ID

FBal0294773

Feature type

allele

Associated gene

Dmel\Itpr

Associated Insertion(s)

Carried in Construct

P{NIG.1063R}

Also Known As

dsitpr, UASitpr^IR, UASdsitpr, itpr-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Kobayashi et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kobayashi et al., 2006)

Mutagen

in vitro construct

(Agrawal et al., 2010)

Progenitor genotype

Carried in construct

P{NIG.1063R}

Cytology

Description

UASt regulatory sequences drive expression of a pair of inverted Itp-r83A cDNA fragments.

(Kobayashi et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-1063R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Agrawal et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | larval stage, with Scer\GAL4^Act5C.PI

(Agrawal et al., 2009)

abnormal flight, with Scer\GAL4^elav-C155

(Richhariya et al., 2017, Agrawal et al., 2010)

abnormal flight, with Scer\GAL4^ple.PF

(Pathak et al., 2015)

abnormal flight, with Scer\GAL4^ple.TH-D

(Pathak et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Kobayashi et al., 2006)

abnormal neurophysiology, with Scer\GAL4^Ddc.PL

(Agrawal et al., 2010)

abnormal neurophysiology, with Scer\GAL4^elav-C155

(Agrawal et al., 2010)

abnormal neurophysiology, with Scer\GAL4^Ilp2.PR

(Agrawal et al., 2010)

abnormal neurophysiology, with Scer\GAL4^ple.PF

(Pathak et al., 2015)

lethal - all die before end of larval stage, with Scer\GAL4^Act5C.PI

(Agrawal et al., 2009)

partially lethal - majority die, with Scer\GAL4^elav-C155

(Agrawal et al., 2010)

some die during P-stage, with Scer\GAL4^elav-C155

(Agrawal et al., 2010)

some die during third instar larval stage, with Scer\GAL4^Act5C.PI

(Agrawal et al., 2009)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Kobayashi et al., 2006)

antennal lobe, with Scer\GAL4^ey-OK107

(Kobayashi et al., 2006)

dorsal longitudinal indirect flight muscle cell, with Scer\GAL4^Trhn.PB

(Sadaf et al., 2012)

wing, with Scer\GAL4^elav-C155

(Agrawal et al., 2010)

Detailed Description

Statement

Reference

Expression of Itp-r83A^NIG.1063R under the control of Scer\GAL4^elav-C155 leads to significant decrease in flight ability in adults (measured as flight time in a single flight assay on tethered flies).

(Richhariya et al., 2017)

A partial flight deficit is observed upon expression of Itp-r83A^NIG.1063R in dopaminergic neurons under the control of Scer\GAL4^ple.PF. The flight deficits correlated with abnormalities in action potentials recorded from dorsal longitudinal muscles during air puff-induced flight bouts.

Pupal expression of Itp-r83A^NIG.1063R in all dopaminergic neurons leads to partial flight deficits.

(Pathak et al., 2015)

Flies expressing Itp-r83A^NIG.1063R under the control of Scer\GAL4^Trh.PB show normal flight ability in a cylinder drop test. However, the flies show increased spontaneous firing from the dorsal longitudinal indirect flight muscles compared to wild type.

(Sadaf et al., 2012)

Pan-neuronal expression of Itp-r83A^NIG.1063R driven by Scer\GAL4^elav-C155 results in partial pupal lethality. The surviving Scer\GAL4^elav-C155; Itp-r83A^NIG.1063R flies exhibit wing posture defects. Scer\GAL4^elav-C155; Itp-r83A^NIG.1063R mutant flies show significant flight defects and are unable to initiate rhythmic flight patterns in recordings from the dorsal longitudinal muscles in response to air-puff stimulus.

No significant change in wing posture or flight ability is observed when Itp-r83A^NIG.1063R is expressed with either Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL.

Spontaneous firing is increased from the dorsal longitudinal muscles when Itp-r83A^NIG.1063R is expressed with either Scer\GAL4^elav-C155 or Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL.

In primary neuronal cultures obtained from brains of larvae expressing Itp-r83A^NIG.1063R driven by Scer\GAL4^elav-C155, store operated Ca2[2+] entry is reduced compared to controls.

Store Ca[2+] or "["Ca[2+]"]"[[ER]] is similar in primary neuronal cultures between brains from larvae expressing Itp-r83A^NIG.1063R driven by Scer\GAL4^elav-C155 and controls.

(Agrawal et al., 2010)

Expression of Itp-r83A^NIG.1063R under the control of Scer\GAL4^Act5C.PI results in significantly smaller larvae compared to controls at 120 hours after egg laying. At an earlier stage, 85 hours after egg laying, mutant larvae are similar in size to control larvae.

Expression of Itp-r83A^NIG.1063R under the control of Scer\GAL4^elav-C155, Scer\GAL4^Ddc.PL or Scer\GAL4^Ilp2.PR (and in the presence of Dcr-2^Scer\UAS.cDa) does not affect larval size, and mutant larvae are similar in size to control larvae, and the number of pupae formed is similar in number to controls.

(Agrawal et al., 2009)

Expression of Itp-r83A^{dsRNA.Scer\UAS} in the mushroom bodies, under the control of Scer\GAL4^OK107 results in defects in the mushroom bodies in approximately 24% of cases (compared to 13% when only Itp-r83A^{dsRNA.Scer\UAS} is present) and defects in the antennal lobes in 5% of cases. No defects were observed in the optic lobes.

(Kobayashi et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Itpr^NIG.1063R, Scer\GAL4^elav-C155 has abnormal flight phenotype, suppressible | partially by Rala^UAS.cRa, Scer\GAL4^elav-C155

(Richhariya et al., 2017)

Itpr^NIG.1063R, Scer\GAL4^ple.PF has abnormal flight phenotype, suppressible by ple^+t10.988

(Pathak et al., 2015)

Itpr^NIG.1063R, Scer\GAL4^ple.TH-D has abnormal flight phenotype, suppressible by ple^UAS.cFa, Scer\GAL4^ple.TH-D

(Pathak et al., 2015)

Other

Statement

Reference

Dcr-2^UAS.cDa, Itpr^NIG.1063R, Scer\GAL4^Ddc.PL, Scer\GAL4^Ilp2.PR has abnormal flight phenotype

(Agrawal et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Itpr^NIG.1063R, Scer\GAL4^elav-C155 has wing phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^elav-C155

(Agrawal et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The severely reduced flight ability (measured as flight time in a single flight assay on tethered flies) characteristic for flies expressing Itp-r83A^NIG.1063R under the control of Scer\GAL4^elav-C155 is significantly ameliorated by co-expression of Rala^Scer\UAS.cRa.

(Richhariya et al., 2017)

ple^+t10.988 can suppress the flight deficit of Scer\GAL4^ple.PF>Itp-r83A^NIG.1063R flies.

Overexpression of ple^Scer\UAS.cFa encoding the neuronal isoform either through development, or in pupae alone, can rescue flight initiation of Scer\GAL4^ple.TH-D>olf186-F^{E180A.Scer\UAS} animals.

(Pathak et al., 2015)

The spread out Scer\GAL4^elav-C155; Itp-r83A^NIG.1063R wing posture defect is further exacerbated on enhancing RNA interference by expressing a Dcr-2^Scer\UAS.cDa transgene.

No significant change in wing posture or flight ability is observed when Itp-r83A^NIG.1063R is expressed with either Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL even when Itp-r83A^NIG.1063R expression is enhanced with a Dcr-2^Scer\UAS.cDa transgene.

Flies expressing Itp-r83A^NIG.1063R and Dcr-2^Scer\UAS.cDa driven by both Scer\GAL4^Ilp2.PR and Scer\GAL4^Ddc.PL show normal wing posture, but they are unable to maintain flight beyond 15-20 s.

(Agrawal et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

1063R-1

P{NIG.1063R}1

1063R-2

P{NIG.1063R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Itp-r83A^NIG.1063R

Itp-r83A^{dsRNA.Scer\UAS}

Itpr^NIG.1063R

Name Synonyms

Secondary FlyBase IDs

FBal0197477
FBal0243768

References (18)

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