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General Information
Symbol
Ctet\tetXIMPTNT-V.UAS
Species
C. tetani
Name
FlyBase ID
FBal0049204
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-IMPTNT, TNT-VIF, UAS-IMPTNT-V, IMPTNT, UAS-TNT-VIF, IMPTNT-VA, UAS-TNT-V, UAS-IMPTNT-VB, UAS-tntIMP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a mutated Ctet\TeTxLC gene fragment (carries the amino acid replacements H233V and H237V).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS in R1 or R1-4 neurons (under the control of Scer\GAL4c105 or Scer\GAL4796) cover significantly less distance than control flies. This is partially due to their diminished walking activity and partially due to their diminished walking speed. Speed is significantly reduced only when R1-4 neurons (Scer\GAL4796) are silenced. The ability of flies with inactivated R1-4 neurons to orientate towards visible landmarks is no different from that of control flies; both groups preferentially keep objects in the frontal visual field. Flies with silenced R1 neurons (Scer\GAL4c105), however, appear to preferentially keep objects under +/-30[o] to +/-40[o] from the front. This is statistically significant.

Flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of Scer\GAL4Trh.PB show normal flight ability in a cylinder drop test.

Air-puff stimulated flight responses of the dorsal longitudinal indirect flight muscle (DLM) of tethered flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of Scer\GAL4Trh.PB are normal.

Males expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of either Scer\GAL4Or65a.4.7 or Scer\GAL4Or65a.PF and treated with cis-vaccenyl acetate (cVa) show an inhibition of initial courtship of mature virgin females, as do wild-type males treated with cVa.

The normal architecture of the CSD interneuron (CSDn) is only slightly altered by targeted expression of Ctet\TeTxLCIMPTNT-V.Scer\UAS driven by Scer\GAL4αTub84B.PP.

Ctet\TeTxLCIMPTNT.Scer\UAS; Scer\GAL4NP1017 adults starved of water for 2 hours a normal proboscis extension response when their prothoracic legs are exposed to water.

Expression of Ctet\TeTxLCIMPTNT-V.Scer\UAS throughout the CNS, using Scer\GAL4elav-C155 as the driver, does not affect embryonic ventral cord condensation.

Expression of Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of Scer\GAL4GMR.PF does not affect 5-HT arborization in larval brains.

When Ctet\TeTxLCIMPTNT-V.Scer\UAS is driven by Scer\GAL4P2.4.Pdf no effect is seen on sensitivity to cocaine.

The olfactory attraction and repulsion behaviour of flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of either Scer\GAL485b or Scer\GAL4oeC in response to various chemicals, including butanol, benzaldehyde, propionic acid and acetone, does not significantly differ from that of wild-type.

Adults expressing Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4ple.PF show a normal response in an assay in which flies are placed in a column with a conic bottom end and then suddenly tapped to the bottom to startle them.

When Ctet\TeTxLCIMPTNT-V.Scer\UAS is driven by Scer\GAL4109(2)80, mutant larvae exhibit a normal noxious heat response.

The average locomotor activity plots in light:dark (LD) cycles of flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of Scer\GAL4per.PK or Scer\GAL4tim.PE is indistinguishable from those of control flies. Expression of Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of Scer\GAL4per.PK under constant darkness conditions does not result in a decrease in rhythmicity of locomotor behaviour. Expression of Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of Scer\GAL4tim.PE under constant darkness conditions results in a decrease in in the number of rhythmic flies in a locomotor activity assay.

When expression is driven by Scer\GAL4c17 in the giant fibre the response in the TTM to repetitive firing is wild type.

When Ctet\TeTxLCIMPTNT-V.Scer\UAS is driven by Scer\GAL4eve.RRC in aCC neurons, excitatory post-synaptic currents (epscs) are present in 14/18 neurons. Sustained inward currents are also seen in 14/18 neurons.

Flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of Scer\GAL4MZ1369 have no obvious developmental defects. The wild-type columnar organisation of the medulla is recognisable in the optic lobes and the arborisations of the Fas2-positive lamina monopolar cells in the distal medulla are normal.

Scer\GAL4 mediated expression has no effect on viability.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

per01 mutant files co-expressing perScer\UAS.cBa and Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4Mai179 display morning anticipation in their locomotor activity, but they completely lose evening anticipation, compared with controls with Ctet\TeTxLCIMPTNT-V.Scer\UAS expression.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Ctet\TeTxLCIMPTNT-V.Scer\UAS
Ctet\TeTxLCIMPTNT-V.UAS
Ctet\tetXIMPTNT-V.UAS
TeTxLCIMPTNT-V.UAS
Name Synonyms
Secondary FlyBase IDs
    References (50)