Flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS in R1 or R1-4 neurons (under the control of Scer\GAL4c105 or Scer\GAL4796) cover significantly less distance than control flies. This is partially due to their diminished walking activity and partially due to their diminished walking speed. Speed is significantly reduced only when R1-4 neurons (Scer\GAL4796) are silenced. The ability of flies with inactivated R1-4 neurons to orientate towards visible landmarks is no different from that of control flies; both groups preferentially keep objects in the frontal visual field. Flies with silenced R1 neurons (Scer\GAL4c105), however, appear to preferentially keep objects under +/-30[o] to +/-40[o] from the front. This is statistically significant.
The olfactory attraction and repulsion behaviour of flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of either Scer\GAL485b or Scer\GAL4oeC in response to various chemicals, including butanol, benzaldehyde, propionic acid and acetone, does not significantly differ from that of wild-type.
Adults expressing Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4ple.PF show a normal response in an assay in which flies are placed in a column with a conic bottom end and then suddenly tapped to the bottom to startle them.
When expression is driven by Scer\GAL4c17 in the giant fibre the response in the TTM to repetitive firing is wild type.
Flies expressing Ctet\TeTxLCIMPTNT-V.Scer\UAS under the control of Scer\GAL4MZ1369 have no obvious developmental defects. The wild-type columnar organisation of the medulla is recognisable in the optic lobes and the arborisations of the Fas2-positive lamina monopolar cells in the distal medulla are normal.
Scer\GAL4 mediated expression has no effect on viability.