FlyBase Allele Report: Dmel\per[UAS.cBa]

General Information

Symbol

Dmel\per^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Blanchardon

FlyBase ID

FBal0140982

Feature type

allele

Associated gene

Dmel\per

Associated Insertion(s)

Carried in Construct

P{UAS-per.B}

Also Known As

UAS-per, uas-per16

Key Links

Transgenic product class

cDNA

(Blanchardon et al., 2001)

wild_type

(Blanchardon et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Blanchardon et al., 2001)

wild_type

(Blanchardon et al., 2001)

Mutagen

in vitro construct

(Grima et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-per.B}

Cytology

Description

UASt regulatory sequences drive expression of a 3.9 kb per cDNA (beginning 46 bp upstream from the ATG and ending at position 4230 in the mRNA, in the 3' untranslated region).

(Blanchardon et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

per

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4¹¹¹⁸

(Blanchardon et al., 2001)

abnormal locomotor rhythm, with Scer\GAL4^Mz520

(Grima et al., 2004)

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Grima et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

per⁰¹ animals expressing per^Scer\UAS.cBa under the control of Scer\GAL4^P2.4.Pdf or Scer\GAL4^Mz520 have a normal morning activity peak during LD conditions but do not have a normal evening activity peak. In DD conditions, show an activity bout in the subjective day that is about 6 hours before the one seen in wild-type animals. per⁰¹ animals expressing per^Scer\UAS.cBa under the control of Scer\GAL4^Mai179 display a robust anticipation of both lights-on and lights off transitions. In DD conditions, per⁰¹ animals expressing per^Scer\UAS.cBa under the control of either Scer\GAL4^P2.4.Pdf or Scer\GAL4^Mz520 and Scer\GAL4^Mai179, exhibit an extended activity bout in the subjective day that starts about 6 hours before wild-type and continuing towards the end of the subjective day.

(Grima et al., 2004)

Scer\GAL4¹¹¹⁸-driven per^Scer\UAS.cBa expression abolishes activity and eclosion rhythms. Scer\GAL4¹¹¹⁸-driven per^Scer\UAS.cBa expression suppresses activity and eclosion rhythms in a per^Scer\UAS.cBa dose dependent manner, as the number of arrhythmic flies strongly increases between the heterozygous Scer\GAL4¹¹¹⁸ per^Scer\UAS.cBa/+ and the homozygous Scer\GAL4¹¹¹⁸ per^Scer\UAS.cBa genotypes. However, anticipation of the lights-off period transition can be be observed in light-dark conditions at a varying degree. No phase advance is observed for the anticipation of of lights-off. In the same line, no weak short-period rhythm could be detected in constant darkness.

No weak short-period weak rhythm can be detected in Scer\GAL4¹¹¹⁸-driven per^Scer\UAS.cBa expressing lines, even during the first days in constant darkness.

(Blanchardon et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

per^UAS.cBa, Scer\GAL4^tim.PE, per⁰¹, per[+] is an enhancer of abnormal circadian rhythm phenotype of cwo^PB9

(Richier et al., 2008)

per^UAS.cBa, per⁰¹, Scer\GAL4^cry.PE, per[+] is an enhancer of abnormal circadian rhythm phenotype of cwo^PB9

(Richier et al., 2008)

Suppressor of

Statement

Reference

per^UAS.cBa/Scer\GAL4^cry.PE is a suppressor | partially of abnormal locomotor rhythm | adult stage phenotype of Lsm12a^Δ6

(Lee et al., 2017)

per^UAS.cBa/Scer\GAL4^P2.4.Pdf is a suppressor of abnormal locomotor rhythm phenotype of tyf^ΔG14151

(Lim et al., 2011)

per^UAS.cBa/Scer\GAL4^P2.4.Pdf is a suppressor of abnormal locomotor rhythm phenotype of tyf^e00614

(Lim et al., 2011)

Other

Statement

Reference

Ctet\tetX^TNT-LC.UAS, Scer\GAL4^Mai179, per⁰¹, per^UAS.cBa has abnormal locomotor rhythm phenotype

(Johard et al., 2009)

Ctet\tetX^TNT-LC.UAS, Scer\GAL4^Mai179, Scer\GAL80^Pdf.PS, per⁰¹, per^UAS.cBa has abnormal locomotor rhythm phenotype

(Johard et al., 2009)

Scer\GAL4^Mai179, Scer\GAL80^Pdf.PS, cry^b, per⁰¹, per^UAS.cBa has abnormal locomotor rhythm phenotype

(Picot et al., 2007)

Scer\GAL4^cry.PE, Scer\GAL80^Pdf.PS, cry^b, per⁰¹, per^UAS.cBa has abnormal locomotor rhythm phenotype

(Picot et al., 2007)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The aberrant long-period circadian locomotor rhythm under free-running constant dark conditions characteristic for Lsm12^Δ6 mutant adults is partially rescued by Scer\GAL4^cry.PE-driven expression of per^Scer\UAS.cBa.

(Lee et al., 2017)

The weakened rhythmicity seen in tyf^ΔG14151 and tyf^e00614 flies under constant darkness conditions is suppressed by expression of per^Scer\UAS.cBa under the control of Scer\GAL4^P2.4.Pdf.

(Lim et al., 2011)

The length of the circadian cycle is increased when per^Scer\UAS.cBa is expressed in homozygous cwo^PB9 flies with one copy of per⁰¹ under the control of either a Scer\GAL4^tim.PE or a Scer\GAL4^cry.PE driver.

(Richier et al., 2008)

In contrast to wild-type animals, per⁰¹; ; cry^b double mutants expressing Scer\GAL80^Pdf.PS and Scer\GAL4^Mai179 driven per^Scer\UAS.cBa display robust locomotor activity rhythms, with a long 25.7h period under constant light conditions.

(Picot et al., 2007)

Xenogenetic Interactions

Statement

Reference

per⁰¹ mutant files co-expressing per^Scer\UAS.cBa and Ctet\TeTxLC^TNT.Scer\UAS under the control of Scer\GAL4^Mai179 display morning anticipation in their locomotor activity, but they completely lose evening anticipation, compared with controls with Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} expression.

Anticipatory activity is absent in the morning and almost completely lost in the evening in per⁰¹ mutants co-expressing per^Scer\UAS.cBa and Ctet\TeTxLC^TNT.Scer\UAS under the control of both Scer\GAL4^Mai179 and Scer\GAL80^Pdf.PS.

(Johard et al., 2009)

Complementation and Rescue Data

Rescues

per^UAS.cBa/Scer\GAL4^tim.PE rescues per⁰¹

(Colonna Romano et al., 2024)

per^UAS.cBa/Scer\GAL4^per.PK rescues per⁰¹

(Colonna Romano et al., 2024)

per^UAS.cBa/Scer\GAL4^repo rescues per⁰¹

(Colonna Romano et al., 2024)

per^UAS.cBa/Scer\GAL4^VT055852 rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR18H11 rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^VT002080 rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^Clk.-856 rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^tim.UAS rescues per⁰¹

(Sekiguchi et al., 2024)

Partially rescues

per^UAS.cBa/Scer\GAL4^P2.4.Pdf partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR79G05 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR14F03 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^VT026011 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR73B06 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR71G01 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR61G12 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^Dvir\Pdf.1.9 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR51H05 partially rescues per⁰¹

(Sekiguchi et al., 2024)

Scer\GAL4^GMR79A11/per^UAS.cBa partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^VT063307 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR93G12 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^VT039429 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^VT027231 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR53E04 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR43D05 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR20A02 partially rescues per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR93D01 partially rescues per⁰¹

(Sekiguchi et al., 2024)

Scer\GAL4^Clk.1.5/per^UAS.cBa partially rescues per⁰¹

(Zhang et al., 2010)

per^UAS.cBa/Scer\GAL4^Mai179 partially rescues per⁰¹

(Rieger et al., 2009)

per^UAS.cBa/Scer\GAL4^tim.PE partially rescues per⁰¹

(Rieger et al., 2009)

per^UAS.cBa, Scer\GAL4^Mai179, Scer\GAL80^Pdf.PS partially rescues per⁰¹

(Picot et al., 2007)

per^UAS.cBa, Scer\GAL4^cry.PE, Scer\GAL80^Pdf.PS partially rescues per⁰¹

(Picot et al., 2007)

per^UAS.cBa/Scer\GAL4^P2.4.Pdf partially rescues per⁰¹

(Grima et al., 2004)

per^UAS.cBa/Scer\GAL4^Mai179 partially rescues per⁰¹

(Grima et al., 2004)

Fails to rescue

Scer\GAL4^Clk.1.5/per^UAS.cBa fails to rescue per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR18F07 fails to rescue per⁰¹

(Sekiguchi et al., 2024)

Scer\GAL4^VT027163/per^UAS.cBa fails to rescue per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR56H10 fails to rescue per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^GMR55E06 fails to rescue per⁰¹

(Sekiguchi et al., 2024)

per^UAS.cBa/Scer\GAL4^P2.4.Pdf fails to rescue per⁰¹

(Picot et al., 2007)

per^UAS.cBa/Scer\GAL4^dimm-929 fails to rescue per⁰¹

(Grima et al., 2004)

Comments

Expression of per^Scer\UAS.cBa under the control of Scer\GAL4^Clk.1.5 partially rescues the circadian locomotor activity defects seen in per⁰¹ flies under light-dark conditions; the rescued flies show a robust anticipation of the lights-on transition (morning peak), but evening anticipation is extremely weak and difficult to distinguish from an increase in locomotor activity also observed in control per⁰¹ flies at the end of the day.

Expression of per^Scer\UAS.cBa under the control of Scer\GAL4^Clk.1.5 in per⁰¹ flies tested under a 12 hours 29[o]C/12 hours 20[o]C thermal cycle in constant darkness rescues the evening activity peak.

(Zhang et al., 2010)

Expression of per^Scer\UAS.cBa under the control of Scer\GAL4^tim.PE (per expression in all clock neurons) in per⁰¹ flies results in animals that show a similar locomotor activity to wild-type flies, except that the rescued flies have a later evening peak under 12:12 hour light-dark conditions and a longer period under constant darkness conditions.

per⁰¹ flies that are expressing per^Scer\UAS.cBa under the control of Scer\GAL4^Mai179 (per expression in the majority of the lateral neurons) show bimodal locomotor activity patterns under 12:12 hour light-dark conditions. The flies show an advanced morning peak of activity. These flies show morning and evening peaks of locomotor activity that shift into night under 12:12 hour light moonlight conditions (under these conditions, a dim light of quarter-moonlight intensity, 0.03 lux, is switched on during the dark phase), although the morning peak is strongly reduced and is not present in all flies. When it is present, it occurs 1.4 hours earlier than in controls. Under constant moonlight conditions, the morning peak is further reduced and is only visible in 50% of the rescued flies. Most of the rescued flies remain rhythmic under constant moonlight or constant darkness conditions.

per⁰¹ flies that are expressing per^Scer\UAS.cBa under the control of Scer\GAL4^Mai179 in the presence of Scer\GAL80^Pdf.PS (this restricts expression of per to the 5th s-LNv and the 3 cry-positive LNd lateral neurons) have a prominent lights-on peak of locomotor activity. The morning peak occurs before lights-on and appears suppressed in most flies, and the average activity increase that occurs before lights-on is not different from non-rescued per⁰¹ flies (although it is possible to determine the phase of the morning peak in individual rescued flies). Under 12:12 hour light moonlight conditions (under these conditions, a dim light of quarter-moonlight intensity, 0.03 lux, is switched on during the dark phase) the rescued flies show morning and evening peaks of locomotor activity that are shifted into the night. Under constant moonlight conditions approximately 50% of the rescued flies remain rhythmic with a wide phase relationship between morning and evening peaks. Under constant darkness conditions, all of the rescued flies become arrhythmic.

(Rieger et al., 2009)

In contrast to wild-type animals, per⁰¹ mutants expressing Scer\GAL4^P2.4.Pdf driven per^Scer\UAS.cBa do display locomotor rhythms under constant darkness.

Similarly to wild-type animals, per⁰¹ mutants expressing Scer\GAL80^Pdf.PS and Scer\GAL4^Mai179 or Scer\GAL4^cry.PE driven per^Scer\UAS.cBa do not display locomotor acivity rhythms under constant darkness.

(Picot et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

per^Scer\UAS.cBa

per^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Blanchardon

(Grima et al., 2002)

Secondary FlyBase IDs

References (22)

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