FlyBase Allele Report: Scer\GAL4[Clk.1.5]

General Information

Symbol

Scer\GAL4^Clk.1.5

Species

S. cerevisiae

Name

FlyBase ID

FBal0362632

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Clk-GAL4.1.5}

Also Known As

Clk4.1M-GAL4, Clk4.1-GAL4, Clk4.5F-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Zhang et al., 2010, Zhang et al., 2010)

Progenitor genotype

Carried in construct

P{Clk-GAL4.1.5}

Cytology

Description

A 1.5kb fragment of genomic DNA extending from -2.0kb to -0.5kb upstream of the Clk transcription start site regulates expression of a GAL4 driver.

(Zhang et al., 2010)

A 1.5kb fragment of Clk genomic DNA extending from -2.0kb to -0.5kb upstream of the Clk transcription start site.

(Zhang et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

Clk

Clk_4.1/4.5

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

DN1p neuron | subset

(Zhang et al., 2010)

DN1p neuron

(Cavanaugh et al., 2014)

DN1 neuron

(Guo et al., 2016)

Additional Information

Statement

Reference

Drives expression in DN1 neurons and DN1p neurons. The 4.1M insertion (BDSC stock #36316) drives expression in DN1p neurons, while the 4.5F insertion (BDSC stock #37526) drives expression in all DN1 neurons.

(FlyBase Curators, 2017)

GFP reconstitution across synaptic partners (GRASP) using ScerGAL4^Clk.4.1M in combination with EcollexA^{Pdf.cSa.T:HsimVP16} shows expression at the most distal part of s-LNv dorsal projections, ventral to DN1p neuron cell bodies.

(Cavanaugh et al., 2014)

ScerGAL4^Clk.4.5F labels 8 to 10 posterior DN1 neurons.

(Zhang et al., 2010)

Marker for

DN1 neuron

(Guo et al., 2016)

DN1p neuron

(Cavanaugh et al., 2014, Zhang et al., 2010)

Reflects expression of

Clk

(Guo et al., 2016, Cavanaugh et al., 2014, Zhang et al., 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | adult stage, with Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal circadian behavior, with na^UAS.cLa

(Zhang et al., 2010)

abnormal circadian behavior | adult stage, with Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

abnormal circadian behavior | adult stage, with Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal circadian rhythm | adult stage, with alka^HMJ23882

(Frenkel et al., 2017)

abnormal circadian rhythm | adult stage, with alka^JF02149

(Frenkel et al., 2017)

abnormal circadian rhythm | adult stage, with Grd^GD2702

(Frenkel et al., 2017)

abnormal circadian rhythm | adult stage, with Grd^HMJ22152

(Frenkel et al., 2017)

abnormal circadian rhythm | adult stage, with SecCl^JF02437

(Frenkel et al., 2017)

abnormal circadian rhythm | adult stage, with SecCl^KK112026

(Frenkel et al., 2017)

abnormal feeding behavior | adult stage, with Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal locomotor rhythm | adult stage, with Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

abnormal locomotor rhythm | adult stage | conditional, with Ctet\tetX^TNT-LC.UAS

(Chen et al., 2015)

abnormal temperature response, with Pdfr^RNAi.UAS.cUa

(Head et al., 2015)

decreased rate of movement | adult stage, with Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

decreased rate of movement | adult stage, with Spyo\Cas9^UAS.P2, tim^{UAS.gRNAx3.pCFD6}

(Saurabh et al., 2024)

lethal, with Hsap\KCNJ2^{10xUAS.EGFP.FLP-OFF}

(Saurabh et al., 2024)

Phenotype Manifest In

adult brain, with Spyo\Cas9^UAS.P2, per^{UAS.gRNAx4.pCFD6}

(Saurabh et al., 2024)

Detailed Description

Statement

Reference

Elimination of molecular clock function (using cyc^{Δ.UAS.Tag:MYC}) in Scer\GAL4^Clk.4.1M cells in adults does not affect the strength of the circadian rhythm of sleep-wake cycles, compared to controls.

(Bulthuis et al., 2019)

Inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^Clk.1.5 cells in adults results in a significantly lower circadian clock entrainment index for locomotor activity rhythms under 25[o] C:27 [o]C temperature cycles in constant light conditions, compared to controls; this inhibition results in a significantly reduced phase-shift, compared to controls, when light-dark entrained flies are moved to dark-dark with 8 h delayed 25[o] C:27 [o]C temperature cycles.

(Chen et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Clk.1.5, alka^JF02149 has abnormal circadian rhythm | adult stage phenotype, enhanceable by Scer\GAL4^Clk.1.5/SecCl^JF02437/Grd^GD2702

(Frenkel et al., 2017)

Scer\GAL4^Clk.1.5, SecCl^JF02437 has abnormal circadian rhythm | adult stage phenotype, enhanceable by Scer\GAL4^Clk.1.5/Grd^GD2702/alka^JF02149