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General Information
Symbol
Dmel\slamwaldo2
Species
D. melanogaster
Name
FlyBase ID
FBal0140929
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

GGA6376277AAA

Amino acid change:

G879K | slam-PB; G879K | slam-PC

Reported amino acid change:

G879K

Comment:

The reported Gly to Lys amino acid change (GGA to AAA) requires nucleotide changes at two positions of the codon. The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: G879K.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant embryos have defects in germ cell migration; about 50% of the germ cells are lost by the end of embryogenesis. slamwaldo1/slamwaldo2 embryos derived from homozygous slamwaldo1 female germline clones (embryos lack both maternal and zygotic slam function) fail to cellularise during nuclear cycle 14. Germ cells form normally during cycle 10 in these embryos, and are later found distributed throughout an otherwise unstructured embryo. Nuclei reach the periphery normally in mutant precellularisation embryos, the primordial germ cells bud normally and the somatic nuclei continue to divide until cycle 14. The first difference from wild-type is seen during the slow phase of cellularisation; membrane invagination is delayed in the mutant embryos, so that at the time when membranes normally enclose each nucleus basally, the incompletely invaginated membranes of the mutant embryos entrap the nuclei as they pinch off basally. The mutant embryos do attempt to gastrulate, but this disrupts the incompletely formed somatic cells and the embryos appear to fall apart and fail to develop further.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
slamwaldo2
Name Synonyms
Secondary FlyBase IDs
    References (1)