FlyBase Allele Report: Dmel\Mkp3[GS801]

General Information

Symbol

Dmel\Mkp3^GS801

Species

D. melanogaster

Name

FlyBase ID

FBal0194675

Feature type

allele

Associated gene

Dmel\Mkp3

Associated Insertion(s)

P{GSV1}Mkp3^GS801

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GSV1}Mkp3^GS801

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GSV1}

misexpression element

Regulatory region(s)

UASt

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

Phenotype Manifest In

dorsal tracheal branch primordium, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

embryonic trachea & cortical actin cytoskeleton, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

ganglionic tracheal branch primordium, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

tracheal branch primordium, with Scer\GAL4^69B

(Cela and Llimargas, 2006)

tracheal branch primordium, with Scer\GAL4^arm.PS

(Cela and Llimargas, 2006)

tracheal branch primordium, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

tracheal branch primordium, with Scer\GAL4^ptc-559.1

(Cela and Llimargas, 2006)

tracheolar cell, with Scer\GAL4^69B

(Cela and Llimargas, 2006)

tracheolar cell, with Scer\GAL4^arm.PS

(Cela and Llimargas, 2006)

tracheolar cell, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

tracheolar cell, with Scer\GAL4^ptc-559.1

(Cela and Llimargas, 2006)

Detailed Description

Statement

Reference

Embryos that express Mkp3^GS801 under the control of Scer\GAL4^btl.PS show normal tracheal development up to stage 13. At stage 14, several primary dorsal branches and ganglionic branches fail to form or contain only one or two cells. Additionally, breaks can be observed using luminal markers in thin unicellular branches, such as the most ventral (lateral trunk posterior) and dorsal branches. Branch breaks increase with time and in late stages of embryogenesis sometimes almost no branches appear continuous. In vivo imaging shows that tracheal cells appear more rounded than in wild-type embryos and that breaks occur due to tracheal cells pulling apart; at first the cells remain connected by long cytoplasmic extensions that can eventually break.

The tracheal tree of Scer\GAL4^btl.PS>Mkp3^GS801 embryos shows few apoptotic cells, indicating that cell death is not the major reason for the branch integrity phenotype.

The tracheal tubes of stage 16 Scer\GAL4^btl.PS>Mkp3^GS801 embryos show a thinner accumulation of cortical actin compared to controls.

The embryonic tracheal phenotype is also observed when Mkp3^GS801 is expressed under the control of Scer\GAL4^arm.PS, Scer\GAL4^ptc-559.1 or Scer\GAL4^69B.

(Cela and Llimargas, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Mkp3^GS801, Scer\GAL4^btl.PS has ganglionic tracheal branch primordium phenotype, enhanceable by rl[+]/rl^10a

(Cela and Llimargas, 2006)

Mkp3^GS801, Scer\GAL4^btl.PS has dorsal tracheal branch primordium phenotype, enhanceable by rl[+]/rl^10a

(Cela and Llimargas, 2006)

Mkp3^GS801, Scer\GAL4^btl.PS has tracheal branch primordium phenotype, enhanceable by rl[+]/rl^10a

(Cela and Llimargas, 2006)

Mkp3^GS801, Scer\GAL4^btl.PS has ganglionic tracheal branch primordium phenotype, enhanceable by btl^LG19/btl[+]

(Cela and Llimargas, 2006)

Mkp3^GS801, Scer\GAL4^btl.PS has dorsal tracheal branch primordium phenotype, enhanceable by btl^LG19/btl[+]

(Cela and Llimargas, 2006)

Suppressed by

Statement

Reference

Mkp3^GS801, Scer\GAL4^btl.PS has dorsal tracheal branch primordium phenotype, suppressible by rl^Sem.cUa.UAS, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

Mkp3^GS801, Scer\GAL4^btl.PS has ganglionic tracheal branch primordium phenotype, suppressible by rl^Sem.cUa.UAS, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

Mkp3^GS801, Scer\GAL4^btl.PS has tracheal branch primordium phenotype, suppressible by rl^Sem.cUa.UAS, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

NOT suppressed by

Statement

Reference

Mkp3^GS801, Scer\GAL4^btl.PS has tracheal branch primordium phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

Mkp3^GS801, Scer\GAL4^btl.PS has ganglionic tracheal branch primordium phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

Mkp3^GS801, Scer\GAL4^btl.PS has dorsal tracheal branch primordium phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The branch integrity defects of Scer\GAL4^btl.PS>Mkp3^GS801 embryos are increased when these embryos have a rl^10a/+ background relative to a wild-type background.

Coexpression of rl^Sem.cUa.UAS suppresses the tracheal branching defects of Scer\GAL4^btl.PS>Mkp3^GS801 embryos.

Expression of Mkp3^GS801 under the control of Scer\GAL4^btl.PS in a btl^LG19/+ background does not affect the penetrance of the Scer\GAL4^btl.PS>Mkp3^GS801 branch integrity phenotype but results in a significant increase in failure of branch formation.

(Cela and Llimargas, 2006)

Xenogenetic Interactions

Statement

Reference

Coexpression of Mkp3^GS801 with BacA\p35^Scer\UAS.cHa, under the control of Scer\GAL4^btl.PS does not suppress the branch integrity and primary branching defects of Mkp3^GS801 embryos.

(Cela and Llimargas, 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Mkp3^GS801

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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