FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\tremF9
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General Information
Symbol
Dmel\tremF9
Species
D. melanogaster
Name
FlyBase ID
FBal0195241
Feature type
allele
Associated gene
Dmel\trem
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Amino acid replacement: P?L.

    (Lake et al., 2007)

    The P to L change affects a conserved residue in the linker region of the expressed protein between the first and second zinc finger domains.

    (Lake et al., 2007)

    Nucleotide substitution: C?T.

    (Lake et al., 2007, Page et al., 2007)

    Amino acid replacement: P299L.

    (Page et al., 2007)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    3R:20,030,261..20,030,261 [-]
    Nucleotide change:

    C20030261T

    Reported nucleotide change:

    C?T

    Amino acid change:

    P299L | trem-PA

    Reported amino acid change:

    P299L

    (Page et al., 2007)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        tremF9 females show frequent meiotic chromosome segregation errors, as assessed by X-chromosome non-disjunction.

        (Lake et al., 2019)

        tremF9 mutant females exhibit increased nondisjunction and a reduction in meiotic recombination. These defects do not appear to be due to defects in meiotic pairing or synaptonemal complex formation. The number of meiotically-induced double stranded breaks is reduced compared to wild type. The nondisjunction phenotype is partially rescued by treatment with X-rays.

        Oocytes from tremF9 mutant females fail to form a single prometaphase spindle with wild type configuration. It is common to see multiple spindles, ranging from 1 to 4 or more. Only 21% of the metaphase of prometaphase figures are normal. The frequency of abnormal spindles falls to 40% upon treatment with X-rays.

        No dorsal appendage defects are observed in eggs from tremF9 mutant females.

        tremF9/tremf05981 mutant females exhibit increased nondisjunction and a reduction in meiotic recombination.

        (Lake et al., 2011)

        tremF9 mutants shows high levels of X chromosome nondisjunction and are defective in both double stand break formation and recombination.

        (Lake et al., 2007)

        tremF9 mutants demonstrate 49.5% X nondisjunction.

        tremF9 mutants display a 90-fold decrease in the total frequency of crossing over.

        (Page et al., 2007)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        NOT suppressed by
        Statement
        Reference

        tremF9 has abnormal meiotic cell cycle phenotype, non-suppressible by spn-BBU

        (Lake et al., 2011)

        tremF9 has abnormal meiotic cell cycle phenotype, non-suppressible by Scer\GAL4VP16.nanos.UTR/mei-P22UASp.Tag:HA

        (Lake et al., 2011)
        Suppressor of
        Statement
        Reference

        tremF9 is a suppressor of abnormal meiotic cell cycle phenotype of okrAA/okrRU

        (Lake et al., 2011)

        tremF9 is a suppressor of female sterile phenotype of spn-BBU

        (Lake et al., 2011)
        Phenotype Manifest In
        Suppressor of
        Statement
        Reference

        tremF9 is a suppressor | partially of karyosome phenotype of okrAA/okrRU

        (Lake et al., 2011)

        tremF9 is a suppressor | partially of dorsal appendage phenotype of okrAA/okrRU

        (Lake et al., 2011)

        tremF9 is a suppressor of dorsal appendage phenotype of spn-BBU

        (Lake et al., 2011)

        tremF9 is a suppressor of karyosome phenotype of spn-BBU

        (Lake et al., 2011)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Expression of mei-P22Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the increase in non-disjunction or reduction in meiotically-induced double stranded breaks seen in tremF9 mutant females.

        tremF9 partially suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in spn-BBU mutant females.

        The dorsal appendage and karyosome defects observed in spn-BBU are almost fully suppressed in the background of tremF9. The sterility seen in spn-BBU females is also partially suppressed, with flies remaining fertile until at least day 10.

        spn-BBU does not suppress the reduced frequency of meiotic recombination seen in tremF9 mutant females.

        tremF9 partially suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in okrAA/okrRU mutant females.

        The karyosome defects observed in okrAA/okrRU are almost completely suppressed in the background of tremF9. The dorsal appendage defects are also partially suppressed.

        (Lake et al., 2011)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Fails to complement

        tremf05981

        (Lake et al., 2007)
        Rescued by

        tremF9 is rescued by trem+t2.7

        (Lake et al., 2011)

        tremF9/tremf05981 is rescued by Scer\GAL4VP16.nanos.UTR, tremf05981

        (Lake et al., 2011)

        tremF9 is rescued by Scer\GAL4VP16.nanos.UTR/tremUASp.cLa

        (Lake et al., 2011)
        Comments

        Expression of trem+t2.7 fully suppresses the meiotic nondisjunction phenotype seen in tremF9 mutants.

        Expression of tremScer\UAS.P\T.cLa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fully suppresses the meiotic nondisjunction phenotype seen in tremF9 mutants.

        Expression from the UAS element in the tremf05981 insertion under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the increase in nondisjunction seen in the tremF9/tremf05981 mutant females.

        (Lake et al., 2011)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer

        Selected as: a meiosis-defective mutation.

        (Lake et al., 2007)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        tremF9
        (Lake et al., 2019, Lake et al., 2011, Lake et al., 2007, Page et al., 2007)
        Name Synonyms
        Secondary FlyBase IDs
          References (4)