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FB2026_01
,
released March 12, 2026
FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.
Mutant Malpighian tubules contain large multilamellar structures and mutlivesicular bodies which are not present in wild-type tubules. The multilamellar structures are often clustered together to form large inclusions with or without electron-dense whorls within.
Homozygous third instar larvae show no defects in neuromuscular junction morphology, but axonal transport defects are detected at a low frequency.
Npc2aunspecified, Npc2bunspecified has lethal | recessive | larval stage phenotype
Npc2aunspecified, Npc2bunspecified has lethal | recessive | pupal stage phenotype
Npc2aunspecified, Npc2bunspecified has increased cell death | adult stage phenotype
Npc2aunspecified, Npc2bunspecified has adult brain phenotype
Npc2aunspecified, Npc2bunspecified has adult tracheal system phenotype
The majority of Npc2aunspecified ; Npc2bunspecified double homozygotes moult to the third instar quite normally (17% die as first or second instar larvae), but they remain in the third instar for 3-6 days (compared to approximately 2 days for wild type). 26% die while still in the third instar stage, while the remaining 57% form pupae. Approximately one-tenth of the pupae develop to the adult stage, but these animals are sick and usually die within 2 weeks.
Expression of Npc2bScer\UAS.cHa under the control of Scer\GAL4tub or Scer\GAL42-286 rescues the lethality seen in Npc2aunspecified ; Npc2bunspecified double homozygotes, such that 83% or 86% respectively of the double mutants survive to adulthood.
Expression of Npc2aScer\UAS.cHa under the control of Scer\GAL4tub or Scer\GAL42-286 rescues the lethality seen in Npc2aunspecified ; Npc2bunspecified double homozygotes, such that 78% of the double mutants survive to adulthood.
7 day old Npc2aunspecified ; Npc2bunspecified double homozygous adults show increased cell death in the brain and in the tracheal cells that extend along the top of the brain compared to wild type.