RpS5b^G5346 homozygous females are sterile and do not complete oogenesis. Ovaries are small. Nurse cells, follicle cells, and oocyte differentiate, but very few egg chambers progress beyond stage 8-9, after which point apoptosis is induced (cleaved caspase-3). Some egg chambers contain more than 16 germ cells with one or two oocytes, some egg chambers fail to separate, while others have 16 germ cells but two oocytes, or a mis-localized oocyte. There are increased numbers of follicle cells, with multi-layering at the posterior of egg chambers. Stage 7 oocytes show polarity defects, as microtubules are improperly aligned and often accumulating by the anterior of the oocyte, instead of at the posterior, and Oskar and Gurken are not properly localized; oocytes also show increased F-actin levels.

RpS5b^G5346 germline clones also show severe oogenesis defects. Many egg chambers have multiple layers of follicle cells, and encapsulation defects are observed.

Ovaries from RpS5b^G5346 homozygotes or germline clones show extensive mitochondria clustering, in contrast to a more dispersed distribution in controls. In most cases, mitochondria are less elongated and have less well resolved cristae; sometimes two mitochondria adhere to each other via the outer membrane, and some mitochondria bend and engulf some cytoplasm, forming a donut shape.

RpS5b^G5346 has abnormal size | oogenesis phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/RpS5a^GL01502

RpS5b^G5346 has increased cell number | oogenesis phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/RpS5a^UASp.Venus