FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\painRNAi.UAS
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General Information
Symbol
Dmel\painRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0241921
Feature type
allele
Associated gene
Dmel\pain
Associated Insertion(s)
Carried in Construct
P{UAS-pain.RNAi}
Also Known As
UAS-pain RNAi
Key Links
Transgenic product class
RNAi_reagent
(Sakai et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Sakai et al., 2009)
Mutagen
in vitro construct
(Sakai et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-pain.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of a transgene containing inverted repeats of partial pain cDNA (nucleotide positions 2280-2780).

(Sakai et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
pain
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The mating success rates of females expressing paindsRNA.Scer\UAS in insulin-producing cells under the control of either Scer\GAL4Ilp2.PR or Scer\GAL4Ilp2.PU are significantly higher than control females, and the time to copulation (TC) after the males have initiated courtship is significantly shorter. Expression of paindsRNA.Scer\UAS in the mushroom body (Scer\GAL4Mef2.247 or Scer\GAL430Y) or ellipsoid body (Scer\GAL4C41 or Scer\GAL4Aph-4-c232) has no effect on either mating success or TC.

      When paindsRNA.Scer\UAS is expressed in insulin-producing cells under the control of Scer\GAL4Ilp2.PR (limited to the adult cells using Scer\GAL80ts.αTub84B) mating success rate and the time to copulation (TC) are reduced compared to controls.

      The number of times females expressing paindsRNA.Scer\UAS under the control of Scer\GAL4Ilp2.PR reject males is significantly lower than that of wild-type females.

      (Sakai et al., 2014)

      Expression of paindsRNA.UAS under the control of Scer\GAL4ey-OK107, Scer\GAL4c747, Scer\GAL430Y, Scer\GAL4Mef2.247 and Scer\GAL4Ilp2.PR but not Scer\GAL4Alp4-c232 or Scer\GAL4OK348 leads to courtship long term memory defect (no reduction in courtship activity 5 days after 7-hours conditioning) when compared to controls.

      (Sakai et al., 2012)

      Time to copulation of paindsRNA.Scer\UAS/Scer\GAL4pain-GAL4 females is significantly shorter than that of control females.

      Time to copulation of paindsRNA.Scer\UAS/Scer\GAL4Cha.7.4 females is significantly shorter than that of control females.

      Time to copulation of paindsRNA.Scer\UAS/Scer\GAL4ple.PF and paindsRNA.Scer\UAS/Scer\GAL4Ddc.PL females is no different than that of control females.

      F1 females between Scer\GAL4Gad1 and paindsRNA.Scer\UAS show enhanced sexual receptivity.

      (Sakai et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      painRNAi.UAS
      paindsRNA.Scer\UAS
      paindsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (4)