FlyBase Allele Report: Dmel\mam[k1514]

General Information

Symbol

Dmel\mam^k1514

Species

D. melanogaster

Name

FlyBase ID

FBal0243521

Feature type

allele

Associated gene

Dmel\mam

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Endo et al., 2007)

Progenitor genotype

Cytology

Description

A single nucleotide substitution in the coding sequence of mam results in a truncated protein of 83 amino acids from the N-terminus.

(Endo et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Endo et al., 2007)

abnormal neuroanatomy | somatic clone

(Joo et al., 2013)

Phenotype Manifest In

adult olfactory receptor neuron Or49a/85f

(Endo et al., 2007)

antennal lobe glomerulus DA1

(Endo et al., 2007)

antennal lobe glomerulus DA3

(Endo et al., 2007)

antennal lobe glomerulus DA4l

(Endo et al., 2007)

antennal lobe glomerulus DC1

(Endo et al., 2007)

antennal lobe glomerulus DL1

(Endo et al., 2007)

antennal lobe glomerulus DL4

(Endo et al., 2007)

antennal lobe glomerulus DM1

(Endo et al., 2007)

antennal lobe glomerulus DM2

(Endo et al., 2007)

antennal lobe glomerulus DM3

(Endo et al., 2007)

antennal lobe glomerulus DM4

(Endo et al., 2007)

antennal lobe glomerulus V

(Endo et al., 2007)

antennal lobe glomerulus VA4

(Endo et al., 2007)

antennal lobe glomerulus VC1

(Endo et al., 2007)

antennal lobe glomerulus VC2

(Endo et al., 2007)

antennal lobe glomerulus VC3

(Endo et al., 2007)

antennal lobe glomerulus VC4

(Endo et al., 2007)

antennal lobe glomerulus VL1

(Endo et al., 2007)

antennal lobe glomerulus VM1

(Endo et al., 2007)

antennal lobe glomerulus VM4

(Endo et al., 2007)

olfactory receptor neuron

(Joo et al., 2013, Endo et al., 2007)

Detailed Description

Statement

Reference

In homozygous mam^k1514 mutant AM29+ paired MARCM clones, both axons adopt a ventromedial trajectory and target to DM6.

(Joo et al., 2013)

In mam^k1514 mutants, the axonal projection to glomerulus DL4 is abolished, while the projection to DM6 remains. mam^k1514 mutants autonomously switch both the axonal pathway and the target glomerulus of the olfactory neurons projecting to DL4 to those of the olfactory neurons projecting to DM6.

In mam^k1514 mutants, the axonal projections to glomeruli VL1, VM4, VC3l, VM1, VC3m, VC5, VC1, VA4, VC2, VC4, DL1, DM1, V, DM4, DM2, DM3, DA4l, DC1, DA3, and DA1 are abolished.

mam^k1514 olfactory organ cell clones induced at 30 hours before puparium formation and analyzed at 20-24 hours after puparium formation contain eight cells that stain with sens indicating they are pNb progeny, suggesting that all the progenies of PIIa and pIIB transform to pNb-like cells.

(Endo et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mam^k1514 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Sema2b^f02042

(Joo et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

mam^k1514 has olfactory receptor neuron | somatic clone phenotype, suppressible | partially by Sema2b^f02042

(Joo et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

In Sema-2b^f02042, mam^k1514 double mutant paired axon clones (generated through expression of Scer\FLP1^hs.PG, Scer\GAL80^ts.αTub84B and Scer\GAL4^AM29), both neurons chose a dorsolateral axon trajectory, a phenotype opposite to that of mam^k1514 paired clones. Sema-2b removal only partially reverts the mam^k1514 phenotype in glomerular targeting, as double-mutant axons skip the DL4 glomerulus in 46% of clones despite taking the correct dorsolateral trajectory that passes by the target DL4 glomerulus.

(Joo et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

109584

w^*; P{FRT(w^hs)}G13 mam^k1514 P{UAS-mCD8::GFP.L}LL5 / SM1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mam^k1514

(Joo et al., 2013, Endo et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close