FlyBase Allele Report: Dmel\Vav[3]

General Information

Symbol

Dmel\Vav³

Species

D. melanogaster

Name

FlyBase ID

FBal0263790

Feature type

allele

Associated gene

Dmel\Vav

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Malartre et al., 2010)

Mutagen

Delta2-3 transposase

(Malartre et al., 2010)

Progenitor genotype

Vav^KG02022

(Malartre et al., 2010)

Cytology

Description

Imprecise excision of vav^KG02022 results in a 2293bp deletion of the vav locus. Neighbouring genes CG8010 and rictor are intact.

(Malartre et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:19,261,906..19,264,198 [+]

Comment:

2293 bp deletion resulting from the imprecise excision of P{SUPor-P}Vav^KG02022. Upstream endpoint at insertion site inferred from map.

(Malartre et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

commissure | embryonic stage 17

(Malartre et al., 2010)

cone cell | pupal stage | increased number

(Martín-Bermudo et al., 2015)

cone cell | pupal stage | somatic clone | increased number

(Martín-Bermudo et al., 2015)

eye disc | third instar larval stage

(Martín-Bermudo et al., 2015)

eye photoreceptor cell | pupal stage | increased number

(Martín-Bermudo et al., 2015)

interommatidial cell | pupal stage | increased number

(Martín-Bermudo et al., 2015)

larval longitudinal connective | embryonic stage 17

(Malartre et al., 2010)

ommatidial precursor cluster | third instar larval stage

(Martín-Bermudo et al., 2015)

photoreceptor | somatic clone | third instar larval stage

(Malartre et al., 2010)

photoreceptor cell R7 | increased number | third instar larval stage

(Martín-Bermudo et al., 2015)

photoreceptor cell R7 | pupal stage | increased number

(Martín-Bermudo et al., 2015)

photoreceptor neuron | increased number | third instar larval stage

(Martín-Bermudo et al., 2015)

primary pigment cell | pupal stage | increased number

(Martín-Bermudo et al., 2015)

primary pigment cell | pupal stage | somatic clone | increased number

(Martín-Bermudo et al., 2015)

retina | pupal stage

(Martín-Bermudo et al., 2015)

retina | pupal stage | somatic clone

(Martín-Bermudo et al., 2015)

Detailed Description

Statement

Reference

Somatic clones (induced specifically in the eye) of Vav³ homozygous cells in pupal retina display supernumerary cells in ommatidia (including cone cells and primary pigment cells).

The regular hexagonal lattice of pupal retina is disorganized in homozygous Vav³ mutants and contain extra cells of all types (inter-ommatidial cells, primary pigment cells as well as cone cells).

Vav³ homozygous mutants display extra photoreceptor cells in third instar larval eye disc (R7 and R8) as well as in the majority of ommatidia (supernumerary R7 photoreceptor cells) in a pupal retina. Precocious recruitment of R7 cells is observed in Vav³ mutant eye discs compared to wild-type controls.

The presence of supernumerary accessory cells in Vav³ homozygous pupal ommatidia does not correlate with the presence of extra R7 photoreceptor cells and the numbers of extra cone cells also do not correlate with the number of primary pigment cells.

Vav³ mutants display defect in ommatidial cluster rotation in third instar larval eye disc.

Cells in Vav³ homozygous somatic clones display defects in adherens junction dynamics and excessive cell movements (revealead by live imaging) which result in a patterning delay. The dynamics defects correlate with the recruitment of extra primary pigment cells.

(Martín-Bermudo et al., 2015)

The majority of vav³ homozygous mutants die at the pupal stages but male escapers exist. The escapers are wild type in appearance but exhibit locomotor defects and die shortly after eclosion. They have impaired spontaneous activity and a "shaking phenotype".

vav³ pupae that fail to eclose contain fully developed flies that exhibit severe locomotor defects.

Defects in axon projection are seen in approximately 14% of stage 17 vav³ embryos. On average two longitudinal axons are misrouted across the midline in each embryo.

The midline glia is present in vav³ embryos.

Induction of clones in the photoreceptors of vav³ third instar larvae leads to axon mistargeting, whereas when clones are induced in the glial cells axon targeting occurs correctly.

(Malartre et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Vav³ has increased cell number | pupal stage phenotype, enhanceable by shg^k03401

(Martín-Bermudo et al., 2015)

Suppressed by

Statement

Reference

Vav³ has increased cell number | pupal stage phenotype, suppressible by spi¹

(Martín-Bermudo et al., 2015)

NOT suppressed by

Statement

Reference

Vav³ has partially lethal - majority die phenotype, non-suppressible by trio^UAS.cBa/Scer\GAL4^arm.PS

(Malartre et al., 2010)

Vav³ has partially lethal - majority die phenotype, non-suppressible by trio^UAS.cBa/Scer\GAL4^αTub84B.PL

(Malartre et al., 2010)

Suppressor of

Statement

Reference

Vav³ is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, aos^UAS.cUa

(Martín-Bermudo et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Vav³ has retina | pupal stage phenotype, enhanceable by shg^k03401

(Martín-Bermudo et al., 2015)

Vav³ has primary pigment cell | pupal stage | increased number phenotype, enhanceable by shg^k03401

(Martín-Bermudo et al., 2015)

NOT Enhanced by

Statement

Reference

Vav³ has cone cell | pupal stage | increased number phenotype, non-enhanceable by shg^k03401

(Martín-Bermudo et al., 2015)

Suppressed by

Statement

Reference

Vav³ has retina | pupal stage phenotype, suppressible by spi¹

(Martín-Bermudo et al., 2015)

Vav³ has cone cell | increased number | pupal stage phenotype, suppressible by spi¹

(Martín-Bermudo et al., 2015)

NOT suppressed by

Statement

Reference

Vav³ has primary pigment cell | increased number | pupal stage phenotype, non-suppressible by spi¹

(Martín-Bermudo et al., 2015)

Enhancer of

Statement

Reference

Vav³/Vav[+] is an enhancer of retina | pupal stage phenotype of Scer\GAL4^GMR.PU, cindr^{RNAi.PC.PD.UAS}

(Martín-Bermudo et al., 2015)

Vav³/Vav[+] is an enhancer of primary pigment cell | pupal stage | increased number phenotype of Scer\GAL4^GMR.PU, cindr^{RNAi.PC.PD.UAS}

(Martín-Bermudo et al., 2015)

Suppressor of

Statement

Reference

Vav³ is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, aos^UAS.cUa

(Martín-Bermudo et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The supernumerary cone cells in pupal retina characteristic for Vav³ allele is almost completely suppressed by combination with spi¹ in heterozygous state but the increased number of primary pigment cells remains unchanged.

The disorganised ommatidial lattice and supernumerary primary pigment cells phenotype characteristic for Vav³ homozygotes is strongly enhanced by combination with shg^k03401 in heterozygous state but the proportion of ommatidia with extra cone cells is not further increased.

The patterning defects and mild supernumerary primary pigment cells phenotype observed in pupal retina of flies expressing cindr^{dsRNA.PC.PD.Scer\UAS} under the control of Scer\GAL4^GMR.PU can be significantly enhanced by combination with Vav³ in heterozygous state.

The small and rough adult eye phenotype seen in flies expressing aos^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PU can be partially restored by combination with Vav³.

(Martín-Bermudo et al., 2015)

Expression of trio^Scer\UAS.cBa under the control of either the Scer\GAL4^arm.PS or the Scer\GAL4^αTub84B.PL driver cannot suppress the semi-lethality seen in vav³ male homozygotes.

(Malartre et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Vav³ is rescued by Scer\GAL4^arm.PS/Vav^UAS.Tag:HA

(Malartre et al., 2010)

Vav³ is rescued by Vav^UAS.Tag:HA/Scer\GAL4^αTub84B.PL

(Malartre et al., 2010)

Partially rescued by

Vav³ is partially rescued by Scer\GAL4^arm.PS/Vav^UAS.Tag:HA

(Malartre et al., 2010)

Comments

Expression of vav^{Scer\UAS.T:Ivir\HA1} under the control of either the Scer\GAL4^arm.PS or the Scer\GAL4^αTub84B.PL driver rescues the semi-lethality seen in vav³ male homozygotes.

Expression of vav^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS partially rescues the midline crossing phenotype seen in vav³ homozygotes.

(Malartre et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98874

y¹ w^* Vav³ P{neoFRT}19A/FM7h, P{eve-lacZ8.0}1; P{GAL4-slbo.2.6}chic⁶⁴⁵⁸, P{UAS-GMA}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Vav³

(Kamiyama et al., 2025, Park et al., 2022)

vav³

(Martín-Bermudo et al., 2015, Malartre et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (4)

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