FlyBase Allele Report: Dmel\Apc2[R1-R5SD.EGFP]

General Information

Symbol

Dmel\Apc2^R1-R5SD.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0268246

Feature type

allele

Associated gene

Dmel\Apc2

Associated Insertion(s)

Carried in Construct

P{Apc2-EGFP.R1-R5SD}

Key Links

Transgenic product class

missense_variant

(Kunttas-Tatli et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kunttas-Tatli et al., 2012)

Mutagen

in vitro construct

(Kunttas-Tatli et al., 2012)

Progenitor genotype

Carried in construct

P{Apc2-EGFP.R1-R5SD}

Cytology

Description

Full length Apc2 containing several amino acid replacements in the 20R1 and 20R5 regions (S610D, D613D, N616D, T619D, S660D, S663D, V669D, S752D, T755D, S758D, S761D, S799D, P802D, S805D, S808D, S877D, S880D, S883D, T886D) is expressed under the control of the native promoter and tagged with EGFP.

(Kunttas-Tatli et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

Apc2

Encoded product / tool

Apc2

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Apc2^R1-R5SD.EGFP is a suppressor | partially of lethal | germline clone | rescuable maternal effect | embryonic stage phenotype of Apc2^g10, Apc^Q8

(Kunttas-Tatli et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Apc2^R1-R5SD.EGFP is a suppressor | partially of embryonic/larval cuticle | germline clone | rescuable maternal effect | embryonic stage phenotype of Apc2^g10, Apc^Q8

(Kunttas-Tatli et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Apc2^{R1-R5SD.T:Avic\GFP-EGFP} partially suppresses the lethality seen in embryos derived from females carrying homozygous Apc2^g10 Apc^Q8 germline clones and heterozygous double mutant males (generated using the FRT-FLP-DFS technique). 49% of embryos are able to hatch. The remaining progeny exhibit a more severe cuticle phenotype than in the double mutant alone.

(Kunttas-Tatli et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Apc2^R1-R5SD.EGFP partially rescues Apc2^g10

(Kunttas-Tatli et al., 2012)

Comments

Expression of Apc2^{R1-R5SD.T:Avic\GFP-EGFP} partially rescues the lethality seen in embryos that are both maternally and zygotically mutant for Apc2^g10, resulting in a 89% hatch rate. Very weak cuticle defects are seen in the embryos that fail to hatch.

(Kunttas-Tatli et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Apc2^R1-R5SD.EGFP

Apc2^{R1-R5SD.T:Avic\GFP-EGFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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