FB2025_01 , released February 20, 2025
Allele: Dmel\Apc2R1-R5ExR.EGFP
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General Information
Symbol
Dmel\Apc2R1-R5ExR.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0268248
Feature type
allele
Associated gene
Dmel\Apc2
Associated Insertion(s)
Carried in Construct
P{Apc2-EGFP.R1-R5ExR}
Key Links
Transgenic product class
missense_variant
(Kunttas-Tatli et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kunttas-Tatli et al., 2012)
Mutagen
in vitro construct
(Kunttas-Tatli et al., 2012)
Progenitor genotype
Carried in construct
P{Apc2-EGFP.R1-R5ExR}
Cytology
Description

Full length Apc2 containing several amino acid replacements in the 20R1 and 20R5 regions (E595Q, Y600A, E603Q, T605A, P606A, T645A, D650A, L653A, T655A, P656A, D737N, F742A, E745Q, T747A, P748A, D784N, Y789A, E792Q, T794A, T795A, D862N, Y867A, E870Q, S872A, T873A) is expressed under the control of the native promoter and tagged with EGFP.

(Kunttas-Tatli et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
Apc2
Encoded product / tool
Apc2
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Apc2R1-R5ExR.T:Avic\GFP-EGFP fails to suppress the lethality seen in embryos derived from females carrying homozygous Apc2g10 ApcQ8 germline clones and heterozygous double mutant males (generated using the FRT-FLP-DFS technique). 21% of embryos are able to hatch. The severity of the cuticle defects in the remaining progeny is similar to the double mutant alone.

      (Kunttas-Tatli et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Apc2R1-R5ExR.EGFP partially rescues Apc2g10

      (Kunttas-Tatli et al., 2012)
      Comments

      Expression of Apc2R1-R5ExR.T:Avic\GFP-EGFP modestly rescues the lethality seen in embryos that are both maternally and zygotically mutant for Apc2g10, resulting in a 34% hatch rate. Weak cuticle defects are seen in the embryos that fail to hatch.

      (Kunttas-Tatli et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Apc2R1-R5ExR.EGFP
      Apc2R1-R5ExR.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)