FB2025_01 , released February 20, 2025
Allele: Dmel\dshY473F.EGFP
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General Information
Symbol
Dmel\dshY473F.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0269799
Feature type
allele
Associated gene
Dmel\dsh
Associated Insertion(s)
Carried in Construct
P{dsh-eGFP.Y473F}
Key Links
Transgenic product class
missense_variant
(Yanfeng et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Yanfeng et al., 2011)
Mutagen
in vitro construct
(Yanfeng et al., 2011)
Progenitor genotype
dshEGFP
(Yanfeng et al., 2011)
Carried in construct
P{dsh-eGFP.Y473F}
Cytology
Description

A 6.7kb genomic fragment containing dsh tagged at the 3' end with EGFP. The tyrosine at residue 473 has been mutated to phenylalanine.

(Yanfeng et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
dsh
Encoded product / tool
dsh
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing dshY473F.T:Avic\GFP-EGFP in a dsh3 mutant background display strong PCP defects in all tissues analysed, including classical PCP defects in the thorax and wings, rough eyes with randomised chirality, symmetrical clusters and misrotation. Co-expression of dshY474F.T:Avic\GFP-EGFP does not enhance these phenotypes.

      (Yanfeng et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Abl2, dshY473F.EGFP, Abl[+] is a suppressor of lethal phenotype of dsh3

      (Yanfeng et al., 2011)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of dshY473F.T:Avic\GFP-EGFP in a Abl2/+ mutant background partially rescues the lethality seen in dsh3. The PCP defects are not rescued.

      (Yanfeng et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      dshY473F.EGFP partially rescues dsh3

      (Yanfeng et al., 2011)
      Fails to rescue

      dshY473F.EGFP fails to rescue dsh1

      (Yanfeng et al., 2011)
      Comments

      Expression of dshY473F.T:Avic\GFP-EGFP partially rescues the lethality seen in dsh3. The PCP defects are not rescued.

      Expression of dshY473F.T:Avic\GFP-EGFP is unable to rescue the PCP defects seen in dsh1 mutants.

      (Yanfeng et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      dshY473F.EGFP
      dshY473F.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)