FlyBase Allele Report: Dmel\SRPK[129-09]

General Information

Symbol

Dmel\SRPK^129-09

Species

D. melanogaster

Name

FlyBase ID

FBal0281702

Feature type

allele

Associated gene

Dmel\SRPK

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

chemical

Nature of the Allele

Allele class

Mutagen

chemical

(Loh et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q520term.

(Loh et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:15,384,654..15,384,654 [+]

Nucleotide change:

C15384654T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q520term

(Loh et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Loh et al., 2012)

female sterile (with Df(2R)ED2426)

(Loh et al., 2012)

female sterile (with Df(2R)ED2436)

(Loh et al., 2012)

female sterile (with Df(2R)Exel9015)

(Loh et al., 2012)

male sterile

(Loh et al., 2012)

viable

(Loh et al., 2012)

viable (with Df(2R)ED2426)

(Loh et al., 2012)

viable (with Df(2R)ED2436)

(Loh et al., 2012)

viable (with Df(2R)Exel9015)

(Loh et al., 2012)

Phenotype Manifest In

karyosome (with Df(2R)ED2426)

(Loh et al., 2012)

karyosome (with Df(2R)ED2436)

(Loh et al., 2012)

karyosome (with Df(2R)Exel9015)

(Loh et al., 2012)

karyosome | germline clone

(Loh et al., 2012)

meiotic spindle (with Df(2R)ED2436)

(Loh et al., 2012)

Detailed Description

Statement

Reference

Homozygotes are sterile, but fully viable without any obvious morphological defects.

Homozygous female germline clones show abnormal karyosome morphology.

The normal spherical karyosome morphology is often disrupted in SRPK^129-09/Df(2R)ED2436 oocytes (79% at stage 6). The karyosome is often deformed, with one or more lobes extending from a mass of chromosomes. In other cases, chromosomes appear separated into multiple masses. Chromosomes sometimes appear to be extensively attached to the nuclear envelope. The karyosome defects are first seen from stage 3 and progressively worsen during oogenesis. Heterochromatin clustering is disrupted in the majority of karyosomes, even those with relatively normal morphology. The chromatin morphology of nurse and follicle cells is normal.

Meiotic spindles of mature SRPK^129-09/Df(2R)ED2436 oocytes are severely defective: only residual microtubules are seen and a proper bipolar spindle structure does not form. In 43% of cases, no or only a few short stubs of microtubules are associated with the chromosomes, in 33% of the cases one or a few longer microtubules are seen and in 23% of cases a partially organised structure is formed.

(Loh et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

SRPK^129-09/Df(2R)ED2436 has karyosome phenotype, non-suppressible by lok^p6/lok^p6

(Loh et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

lok^p6 does not rescue the karyosome defects seen in SRPK^129-09/Df(2R)ED2436 mutant oocytes.

(Loh et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(2R)Exel9015/SRPK^129-09 is rescued by SRPK^{+tCH322-130M04}

(Loh et al., 2012)

Comments

SRPK^{+tCH322-130M04} fully rescues the sterility and cytological defects of SRPK^129-09/Df(2R)Exel9015 mutants.

(Loh et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99714

w^*; SRPK^129-09 c¹ px¹ speck¹/SM1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

2R-129-09

(Loh et al., 2012)

SRPK^129-09

srpk^129-09

(Loh et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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