FlyBase Allele Report: Dmel\PolH[Exc2.15]

General Information

Symbol

Dmel\PolH^Exc2.15

Species

D. melanogaster

Name

FlyBase ID

FBal0296082

Feature type

allele

Associated gene

Dmel\PolH

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Wallace et al., 2014)

Progenitor genotype

P{EPgy2}PolH^EY07711

(Wallace et al., 2014)

Cytology

Description

Imprecise excision of the P{EPgy2}DNApol-η^EY07711 progenitor insertion, resulting in a 1309bp deletion which removes part of the 5'UTR and exons encoding residues 1-436 of DNApol-η.

(Wallace et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:21,940,350..21,941,658 [-]

Comment:

Inferred boundaries of 1309 bp deletion resulting from the imprecise excsion of P{EPgy2}PolH^EY07711. The deletion is reported to remove part of the 5' UTR and residues 1-436 of PolH.

(Wallace et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of xeroderma pigmentosum

CEA

(Wallace et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of xeroderma pigmentosum

is ameliorated by lok^p6

(Wallace et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive | larval stage

(Wallace et al., 2014)

fertile

(Wallace et al., 2014)

fertile (with Df(3L)BSC284)

(Wallace et al., 2014)

radiation sensitive | maternal effect

(Wallace et al., 2014)

radiation sensitive | maternal effect (with Df(3L)BSC284)

(Wallace et al., 2014)

viable

(Wallace et al., 2014)

viable (with Df(3L)BSC284)

(Wallace et al., 2014)

Phenotype Manifest In

mitotic spindle | maternal effect

(Wallace et al., 2014)

mitotic spindle | maternal effect (with Df(3L)BSC284)

(Wallace et al., 2014)

Detailed Description

Statement

Reference

Homozygous DNApol-η^Exc2.15 and DNApol-η^Exc2.15/Df(3L)BSC284 females are viable and fertile, producing embryos with normal hatching rates.

Embryos derived from homozygous DNApol-η^Exc2.15 or DNApol-η^Exc2.15/Df(3L)BSC284 females are significantly more sensitive to exposure to UV irradiation compared to controls. The mutant embryos show a significant increase in mitotic index upon exposure to UV irradiation (this increase is not seen in wild-type controls).

Approximately 20% of embryos derived from homozygous DNApol-η^Exc2.15 or DNApol-η^Exc2.15/Df(3L)BSC284 females have a majority of abnormal (nopo mutant-like) mitotic spindles during syncytial embryogenesis. The frequency of syncytial embryos with a majority of abnormal spindles increases to approximately 90% after exposure to irradiation in the mutant embryos (compared to only 24% in UV-irradiated wild-type controls).

Homozygous larvae are sensitive to treatment with hydroxyurea.

(Wallace et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

PolH^Exc2.15 has mitotic spindle | maternal effect phenotype, suppressible | maternal effect by lok^p6/lok^p6

(Wallace et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The mild abnormal spindle phenotype seen in syncytial embryos derived from homozygous DNApol-η^Exc2.15 females in the absence of UV irradiation is suppressed if the females are also homozygous for lok^p6. In addition, the spindle abnormalities seen after irradiation in embryos derived from homozygous DNApol-η^Exc2.15 females are also suppressed in embryos derived from double mutant females.

(Wallace et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PolH^Exc2.15 is rescued by Scer\GAL4^{VP16.nanos.UTR}/PolH^UASp.Tag:HA

(Wallace et al., 2014)

Df(3L)BSC284/PolH^Exc2.15 is rescued by Scer\GAL4^{VP16.nanos.UTR}/PolH^UASp.Tag:HA

(Wallace et al., 2014)

Comments

Expression of DNApol-η^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the increased sensitivity to UV irradiation and abnormal spindle phenotype seen in embryos derived from homozygous DNApol-η^Exc2.15 or DNApol-η^Exc2.15/Df(3L)BSC284 females.

(Wallace et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

57341

w¹¹¹⁸; PolH^Exc2.15

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DNApol-η^Exc2.15

PolH^Exc2.15

dPolη^Exc2.15

(Wallace et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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